LOCUS       sequence-478375-       11397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, 
            and inactive Pex22 peroxisomal chauffeur as negative control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11397)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1429
FEATURES             Location/Qualifiers
     source          1..11397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3394..3807
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      5367..5554
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5422..5446)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        5898..6311
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6445..7152
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             7670..8473
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9115..9930)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9115..9930)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9838..9857
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9922..9941
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10155..10743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10644..10663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatgtggaac tcactaaaag cattcgctct agtgttcgga ggttgttgct
      781 ccaatgttat tacatttgaa accttaatga gcaacgaaac aggcagcatt aataatctca
      841 ttactttttg tcaattttta tttgttacat gtcagggact ccccgaattt ctggatgttc
      901 atcaaccttt cccttacttc aagcctctga aaactccact tcacgtttat gtcattactg
      961 tcgttttatt ttatatttca tctacgacga ataataacgt atttaaatac aacatatcca
     1021 ttccgattca tatcgttttc agatgcttcg gaaccgtaat aaccatgttt acatgctggt
     1081 tattaaatgg taggaaatat acaaagattc agattttatc aacactattt ttaaccattg
     1141 gagctataat tgcctcatta ttcaaagatg ctgattttcg gtaccaagat ttgaaactgc
     1201 aagcgtggaa aattggaagt gaccaatctg tagatctcac ttttattttt ggcatttgta
     1261 tactagtact ttcatcattc acatcatctt tactttcagc atacaatgaa cgtacgtatc
     1321 aaaaatatgg aaagcattgg aaggagaata tcttttacag tcatttttta tcattacctc
     1381 tctttctatt tagtcgaaag cagttaattc acgaatatcg agtgatgaga aagtctgaaa
     1441 gaatattgtg ttcaaatttt ggagggaaga ttctagtccc gcgagaagaa actctccttc
     1501 ttttcaatgt gttaacacaa tatttttgcg tcaagggtgt taatatcctc gccagtaaaa
     1561 caaacgctct gacactttcc ataacattac ttgtaagaaa atttataagt cttttactga
     1621 gcgtccgact tttcgataat aacttgtcat atactggcta catcggggtc tacctagtct
     1681 tttttggtgc ctttatatac tcgctgggat caattcaccc caggcaaaat gacaaggggg
     1741 cgataaagaa aagtaaaggt tctgtttcta aaggtgaaga attattcact ggtgttgtcc
     1801 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
     1861 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
     1921 tgccagttcc atggccaacc ttagtcacta ctttatcttg gggtgttcaa tgttttgcaa
     1981 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
     2041 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
     2101 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
     2161 atggtaacat tttaggtcac aaattggaat acaattattt ctctgacaat gtttacatca
     2221 ctgctgacaa acaaaagaat ggtatcaaag ctaacttcaa aattagacac aacattgaag
     2281 atggtggtgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
     2341 tcttgttacc agacaaccat tacttatcca ctcaatctaa gttatccaaa gatccaaacg
     2401 aaaagaggga ccacatggtc ttgttagaat ttgttactgc tgctggtatt accttgggta
     2461 tggatgaatt gtacaaagga tccgcagagt atgtgcgggc cctgtttgac tttaatggga
     2521 atgatgaaga agaccttccc tttaagaaag gagacatcct gagaatccgg gataagcctg
     2581 aagagcagtg gtggaatgca gaggacagcg aaggaaagag ggggatgatt cctgtccctt
     2641 acgtggagaa gtatggatct gggagcggat ctggaggcag catggcggaa tatgtacgag
     2701 ctctttttga ttttaatggc aatgatgagg aggatctccc ttttaaaaag ggcgatattc
     2761 tgcgcattcg tgataagcca gaggaacaat ggtggaacgc cgaggatagt gaggggaaac
     2821 gcggcatgat tccggtgccg tatgtagaga agtactaatg gcgccaattg gtgcggcaat
     2881 tgataataac gaaaatgtct tttaatgatc tgggtataat gaggaatttt ccgaacgttt
     2941 ttactttata tatatatata catgtaacat atattctata cgctatagag aaaggaaatt
     3001 tttcaattaa aaaaaaaata gagaaagagt ttcacttctt gattatcgct aacactaatg
     3061 gttgaagtac tgctacttta attttatgct gccaaaccag atgtcaacac agctacaacg
     3121 ccttgccaac agggagttct tcagagacat ggaggctcaa aacgaaatta ttgacagcct
     3181 agacatcaat agtcatacaa cagaaagcga ccacccaact ttggctgata atagcgtata
     3241 aacaatgcat actttgtacg ttcaaaatac aatgcagtag atatatttat gcatattaca
     3301 tataatacat atcacatagg aagcaacagg cgcgttggac ttttaatttt cgaggaccgc
     3361 gaatccttac atcacaccca atcccccaca agtgatcccc cacacaccat agcttcaaaa
     3421 tgtttctact ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac
     3481 ttcaaaacac ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt
     3541 aattacccgt actaaaggtt tggaaaagaa aaaagacacc gcctcgtttc tttttcttcg
     3601 tcgaaaaagg caataaaaat ttttatcacg tttctttttc ttgaaaattt ttttttttga
     3661 tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt catcaatttc
     3721 tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag
     3781 aaagaaagca tagcaatcta atctaagttt taattacaaa agatctatgc tcatagaaaa
     3841 cactaatgat cggtttggta tcgtcataga tgcagggtct tcgggttcga gaatccatgt
     3901 gtttaagtgg caggacacgg aatcgcttct tcatgcaaca aaccaggact cacagtccat
     3961 attacaatca gtacctcata ttcatcaaga aaaagactgg acttttaagc tgaatccagg
     4021 cttgtcgagt tttgaaaaaa aacctcaaga tgcgtacaaa tctcacatca agccgttact
     4081 agattttgct aagaatatta tccctgaatc acattggtcg agttgtcccg tttttattca
     4141 agcaaccgcg ggcatgcgcc ttttacctca agacatacaa tcttccattt tggatggttt
     4201 gtgtcagggg ctcaaacacc ctgcagaatt tttggttgag gattgctcag cacaaattca
     4261 agtcattgat ggtgaaaccg aaggtttata tggctggctt ggcttaaact atctatatgg
     4321 acactttaat gactataatc cagaagtctc tgaccatttt acatttgggt ttatggacat
     4381 gggcggtgcc tctactcaga ttgcgtttgc accgcatgat tcaggagaaa tagctagaca
     4441 tagagatgac atcgccacca tcttcttgag gagtgttaac ggagatttgc agaaatggga
     4501 cgtttttgtt agtacgtggt tagggtttgg tgccaatcag gctagaagaa ggtacttggc
     4561 tcagttgatc aatacccttc cagaaaacac aaatgattac gaaaatgatg acttctctac
     4621 aaggaacttg aatgatccat gtatgccgcg aggaagcagc acggattttg aatttaaaga
     4681 taccatattt cacatcgcgg gttctgggaa ttacgaacaa tgtacaaaat ctatttatcc
     4741 gttactttta aagaacatgc cttgtgatga cgagccgtgc ttgtttaatg gtgttcatgc
     4801 tcctcgaata gactttgcca atgacaaatt tataggtact tctgagtact ggtacactgc
     4861 caacgacgta ttcaaacttg ggggcgaata caactttgac aaatttagca aaagcctaag
     4921 ggagttttgc aattccaatt ggacgcaaat attagcgaac agtgataaag gcgtgtataa
     4981 ttctattccg gagaattttt tgaaagatgc atgctttaag ggcaattggg tccttaatat
     5041 acttcatgaa gggtttgata tgcctcgaat agatgtcgac gcagaaaatg tcaatgacag
     5101 gcccttattt caaagtgtgg aaaaagtcga agaacgagag ttatcgtgga cactaggcag
     5161 aattttactt tatgcctcag gtagcatatt ggcaggtaat gatgacttca tggtgggtat
     5221 tgcgcccagt gaaagaagaa ctaagctcac tggtaagaaa ttcatacctg gcaagttact
     5281 ggagtctgat caactacgca agcaaagttc cggatccggt tcattgggaa gaggtagaag
     5341 atccaaattg taataactcg agtggcgcga atttcttatg atttatgatt tttattatta
     5401 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     5461 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc
     5521 atgaggtcgc tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg
     5581 ctccccattt cgctggatgc acacactggc ttaagatgac aacgccttgc caacagggag
     5641 ttcttcagag acatggaggc tcaaaacgaa attattgaca gcctagacat caatagtcat
     5701 acaacagaaa gcgaccaccc aactttggct gataatagcg tataaacaat gcatactttg
     5761 tacgttcaaa atacaatgca gtagatatat ttatgcatat tacatataat acatatcaca
     5821 taggaagcaa caggcgcgtt ggacttttaa ttttcgagga ccgcgaatcc ttacatcaca
     5881 cccaatcccc cacaagtgat cccccacaca ccatagcttc aaaatgtttc tactcctttt
     5941 ttactcttcc agattttctc ggactccgcg catcgccgta ccacttcaaa acacccaagc
     6001 acagcatact aaatttcccc tctttcttcc tctagggtgt cgttaattac ccgtactaaa
     6061 ggtttggaaa agaaaaaaga caccgcctcg tttctttttc ttcgtcgaaa aaggcaataa
     6121 aaatttttat cacgtttctt tttcttgaaa attttttttt ttgatttttt tctctttcga
     6181 tgacctccca ttgatattta agttaataaa cggtcatcaa tttctcaagt ttcagtttca
     6241 tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa
     6301 tctaatctaa gttttaatta caaaagatct atgccaccac catcaagaag tagaataaac
     6361 aaaacaagaa cattaggaat agtgggtaca gctatagcag tgttggtcac gtcctactat
     6421 atatatcaaa aggtgacagg ttctgtgtcc aaaggagagg agttaatcaa ggaaaacatg
     6481 agaatgaaag ttgtcatgga gggctccgtt aatggtcacc aattcaagtg tacaggggaa
     6541 ggtgaaggta atccttacat gggtacacaa actatgagaa ttaaagtaat tgaaggcgga
     6601 ccactaccat ttgcatttga cattctggca acgtcattca tgtacggatc acgaactttc
     6661 atcaagtacc ctaaaggtat accagacttt ttcaagcaat cttttccaga gggttttaca
     6721 tgggaaaggg ttacaagata cgaagatggg ggtgtcgtca cagttatgca agatacttca
     6781 ttagaagatg gctgccttgt ctatcatgtg caagtaagag gggtgaattt tccttctaac
     6841 ggacctgtga tgcagaaaaa gaccaaaggt tgggaaccaa atactgaaat gatgtaccca
     6901 gctgatggag gtttgagagg ctacacacac atggcgctta aagttgatgg tggaggtcat
     6961 ttgtcttgta gttttgttac cacttatcgt tctaaaaaga ctgttggcaa tatcaaaatg
     7021 ccaggaatac atgctgtaga ccacagacta gaaagactcg aagagagcga taacgaaatg
     7081 ttcgttgtac agagagagca tgccgtagcc aaatttgctg gcttaggcgg tggtatggat
     7141 gaattgtata agggatccgg gtcaggctcc ggatctggcc ctcccgcagc tcttgcgcca
     7201 aaaagacgaa ggtaatggca gtgcttttaa ctaagaatta ttagtctttt ctgcttattt
     7261 tttcatcata gtttagaaca ctttatatta acgaatagtt tatgaatcta tttaggttta
     7321 aaaattgata cagttttata agttactttt tcaaagactc gtgctgtcta ttgcataatg
     7381 cactggaagg ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa
     7441 aaatgctgag cagttacaga gatgttacga accactagtg cactgcagta cacggtttcc
     7501 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
     7561 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
     7621 cttaacccaa ctgcacagaa caaaaacgtg caggaaacga agataaatca tgtcgaaagc
     7681 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
     7741 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
     7801 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
     7861 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
     7921 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
     7981 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
     8041 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
     8101 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
     8161 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
     8221 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     8281 gggtttagat gacaagggag atgcattggg tcaacagtat agaaccgtgg atgatgtggt
     8341 ttctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     8401 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     8461 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     8521 agcttcaatt taattatatc agttattacc cgagtagagc acttgaatcc actgccccgg
     8581 gaatctcggt cgtaatgatt tctataatga cgaaaaaaaa aaaattggaa agaaaaagct
     8641 tcatggcctt tataaaaagg aactatccaa tacctcgcca gaaccaagta acagtatttt
     8701 acggggcaca aatcaagaac aataagacag gactgtaaag atggacgcat tgaactccaa
     8761 agaacaacaa gagttccaaa aagtagtgga acaaaagcaa atgaaggatt tcatgcgttt
     8821 gtactctaat ctggtagaaa gatgtttcac agactgtgtc aatgacttca caacatcaaa
     8881 gctaaccaat aaggaacaaa catgcatcat gaagtgctca gaaaagttct tgaagcatag
     8941 cgaacgtgta gggcagcgtt tccaagaaca aaacgctgcc ttgggacaag gcttgggccg
     9001 ataaggtgta ctggcgtata tatatctaat tatgtatctc tggtgtagcc catttttagc
     9061 atgtaaatat aaagaccgag cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa
     9121 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     9181 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     9241 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     9301 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     9361 cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt
     9421 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     9481 agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     9541 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     9601 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     9661 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     9721 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     9781 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     9841 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga
     9901 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     9961 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
    10021 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagtc
    10081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10261 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggccgc caatgctaaa
    10861 ttcgagtgaa acacaggaag atcagaaaat cctcatttca tccatattaa caataatttc
    10921 aaatgtttat ttgcattatt tgaaactagg caagacaagc aacgaaacgt ttttgaaaat
    10981 tttgagtatt ttcaataaat ttgtagagga ctcagatatt gaaaaaaagc tacagcaatt
    11041 aatacttgat aagaagagta ttgagaaggg caacggttca tcatctcatg gatctgcaca
    11101 tgaacaaaca ccagagtcaa acgacgttga aattgaggct actgcgccaa ttgatgacaa
    11161 tacagacgat gataacaaac cgaagttatc tgatgtagaa aaggattaaa gatgctaaga
    11221 gatagtgatg atatttcata aataatgtaa ttctatatat gttaattacc ttttttgcga
    11281 ggcatattta tggtgaagga taagttttga ccatcaaaga aggttaatgt ggctgtggtt
    11341 tcagggtcca taaagcccac atggataaca ttacccctga attcgcatct agactga
//
