LOCUS       sequence-478378-        3914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3h for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3914)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3h
FEATURES             Location/Qualifiers
     source          1..3914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1471..1605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1508..1527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1562..1581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1726..1891
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2081..2669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2570..2589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2804..3027
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3094..3627)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3816..3821,3822..3838,3839..3844))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatggc ttccagaaag gagggtacag gatctactgc tacttccagc
      301 tcttccacag ctggagctgc tggtaaaggt aaaggcaagg gaggttctgg tgacagcgct
      361 gttaagcagg tccaaatcga cggtctggtg gtgctgaaga tcatcaagca ttaccaggaa
      421 gagggtcaag gtactgaagt tgtgcagggt gtgctcctgg gtctggtggt cgaggataga
      481 ctggagatca ccaactgttt ccctttccct cagcataccg aagatgatgc cgacttcgat
      541 gaggtccagt atcaaatgga gatgatgcgc agcctgaggc atgtcaacat cgaccatctg
      601 catgtgggat ggtatcaatc cacatactat ggctcctttg ttactagggc tctcctggac
      661 tctcagttct cttaccaaca cgctattgag gaatctgtgg tgctgatcta cgatcccatc
      721 aagactgctc agggatctct ctccctcaag gcatatcgcc tgactcctaa gctgatggag
      781 gtctgcaaag agaaggactt cagccctgaa gctctcaaga aggcaaacat tacattcgag
      841 tacatgtttg aagaggtgcc tatcgttatc aagaactccc acctcatcaa cgtcctgatg
      901 tgggagctgg agaagaagtc tgctgttgct gacaagcatg aactgctgag cctcgcttcc
      961 tccaaccatc tcggaaagaa cctgcagctc ctcatggaca gggttgatga aatgagccaa
     1021 gacatcgtga agtacaacac ctacatgagg aatacctcca agcagcagca gcagaaacat
     1081 caataccaac aaagaagaca acaggagaac atgcaacgcc agagcagagg cgaacctcca
     1141 ctccctgaag aggatctgtc caagctgttc aagccacctc agccacctgc tagaatggac
     1201 tctctgctga tcgcaggcca gatcaatacc tattgtcaga acatcaagga gttcactgcc
     1261 cagaacctcg gtaagctgtt catggcacag gcactccaag aatacaacaa ttaaggtacc
     1321 tctagagcct gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat
     1381 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     1441 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     1501 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1561 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
     1621 cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1681 tgagcaataa ctatcataac ccctagggta tacccatcta attggaacca gataagtgaa
     1741 atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc
     1801 agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc
     1861 tcccagttcc caactatttt gtccgcccac aaccggttga cttgggtcaa ctgtcagacc
     1921 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1981 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattgacttg
     2761 ggtcgctctt cctgtggatg cgcagatgcc ctgcgtaagc gggtgtgggc ggacaataaa
     2821 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
     2881 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
     2941 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
     3001 ggccaagggc atgtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc
     3061 ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc
     3121 aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc
     3181 accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag
     3241 gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc
     3301 atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc
     3361 gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa
     3421 gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc
     3481 acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca
     3541 tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg
     3601 cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca
     3661 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat
     3721 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
     3781 accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg
     3841 tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca
     3901 gcagcgaagt cgcc
//
