LOCUS       sequence-478379-        7097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3a for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7097)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3a-TEV-FLAG
FEATURES             Location/Qualifiers
     source          1..7097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4429..4494
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4654..4788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4691..4710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4745..4764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4909..5074
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5264..5852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5753..5772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  5987..6210
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6277..6810)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6999..7004,7005..7021,7022..7027))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgcc agcctacttt caaagacctg agaacgctct gaagagagct
      301 aatgagtttc tggaggtggg caagaagcaa ccagcactgg acgttctcta tgatgtgatg
      361 aagtctaaga agcaccgcac ctggcagaag atccatgaac caatcatgct gaagtacctg
      421 gaactgtgcg tcgatctgcg caagagccac ctcgctaaag agggtctgta tcaatacaag
      481 aacatctgcc aacaggtgaa catcaaatct ctcgaagatg tcgtgagggc ttatctcaag
      541 atggctgagg agaagaccga agctgctaaa gaggagtccc agcagatggt cctggacatc
      601 gaggatctcg ataacattca gactcctgag tctgtgctgc tgtctgccgt gagcggagaa
      661 gatacccaag acaggaccga taggctgctc ctcacaccct gggtgaagtt tctctgggag
      721 tcttacaggc agtgcctcga cctcctgcgc aacaacagca gagtggaaag actgtaccac
      781 gacattgccc agcaagcctt caagttctgt ctgcaataca ctagaaaggc agagttcaga
      841 aagctctgcg ataacctcag gatgcacctg tctcagatcc agagacatca caaccaaagc
      901 accgcaatca atctgaacaa cccagagagc cagtctatgc acctggagac tagactcgtc
      961 caactggact ctgcaatcag catggagctg tggcaggagg ctttcaaggc tgtggaggac
     1021 atccacggac tgttctccct gagcaagaag ccacccaagc ctcagctgat ggcaaactac
     1081 tacaacaagg ttagcactgt gttctggaag tccggaaacg ctctcttcca tgcttctacc
     1141 ctccacaggc tgtatcatct gtccagagaa atgcgcaaga acctgaccca agatgaaatg
     1201 cagagaatga gcactcgcgt gctcctcgcc accctgtcta tccctatcac tcctgagcgc
     1261 accgacatcg ctagactgct cgacatggac ggtatcatcg tggagaagca gagaaggctg
     1321 gccaccctcc tcggtctgca agctcctccc actagaattg gcctgatcaa cgacatggtg
     1381 cgcttcaacg tgctccagta tgtcgtccca gaagtgaagg acctctacaa ttggctggag
     1441 gtggagttca acccactgaa gctctgcgaa agggtcacaa aggtcctgaa ctgggtgaga
     1501 gagcagccag agaaagaacc agagctgcag cagtacgtcc ctcagctcca gaacaacacc
     1561 atcctgaggc tgctgcagca agtgtctcaa atctaccagt ccatcgagtt tagcaggctg
     1621 acctccctgg tgccatttgt ggacgctttc cagctcgaaa gagccattgt ggacgcagct
     1681 agacactgcg atctgcaggt ccgcattgat cacacatcta ggaccctcag cttcggctcc
     1741 gacctcaact acgctaccag agaggatgct cccatcggac cacacctcca atccatgccc
     1801 tccgagcaga ttagaaatca actcaccgct atgtcctccg tcctggcaaa ggctctggag
     1861 gtcatcaagc ctgctcatat cctccaggag aaggaggagc agcaccagct cgctgtcact
     1921 gcctatctga agaactctcg caaggagcat cagagaatcc tggcaagaag acaaaccatc
     1981 gaagagagaa aggagagact ggagtctctg aacatccaga gggagaaaga ggagctggaa
     2041 cagagggagg cagaactcca gaaagttcgc aaagccgaag aggagaggct caggcaggag
     2101 gcaaaggaga gagagaaaga aaggattctg caagaacatg agcagatcaa gaagaagaca
     2161 gttagggaga gactggagca gatcaagaag acagagctgg gtgccaaggc tttcaaggac
     2221 atcgacatcg aggacctgga ggaactggac ccagacttca tcatggctaa gcaggtcgag
     2281 caactggaga aggagaagaa ggaactgcag gaaagactga agaaccaaga gaagaagatt
     2341 gattacttcg agagggctaa acgcctggag gaaatcccac tgatcaagtc tgcttacgag
     2401 gagcagagga tcaaagatat ggatctctgg gagcagcagg aggaggagag aatcaccacc
     2461 atgcagctcg aaagagagaa ggctctggag cacaagaacc gcatgagcag gatgctcgaa
     2521 gatagggacc tgttcgttat gaggctgaag gcagctaggc agtctgtcta cgaggagaaa
     2581 ctgaaacagt tcgaagagag actggcagag gaacgccata acagactgga ggagagaaag
     2641 aggcaaagga aggaggagcg cagaatcacc tactacaggg agaaagaaga agaggagcaa
     2701 agaagggctg aagaacagat gctcaaggag agagaggaga gagaaagggc tgagagagcc
     2761 aaacgcgagg aagagctgag ggaataccaa gagagggtta agaagctcga agaagtcgag
     2821 cgcaagaaga ggcaacgcga actggagatc gaagaaagag agaggaggag agaggaagag
     2881 cgcaggctgg gagactcctc cctgagcagg aaggactcca gatggggaga tagggatagc
     2941 gaaggcactt ggagaaaggg acccgaagct gatagcgagt ggagaagagg accaccagag
     3001 aaggaatgga ggagaggtga gggcagggac gaggacagat cccatcgcag ggacgaagaa
     3061 aggccacgca ggctcggaga tgacgaggat agggagccat ccctgaggcc agatgacgat
     3121 agagtgccca gaagaggtat ggacgatgat agaggaccaa gaagaggtcc cgaggaggat
     3181 cgcttctcca ggagaggagc tgatgatgat aggccaagct ggaggaatac agacgatgat
     3241 agaccaccac gcaggattgc cgatgaggac agaggtaact ggagacacgc tgatgatgat
     3301 aggcctccac gcagaggtct ggatgaggat agaggatctt ggagaaccgc tgacgaagac
     3361 agaggacctc gcagaggtat ggacgatgac agaggaccca gaagaggagg tgcagatgac
     3421 gaacgcagct cttggaggaa cgctgatgat gacagaggtc ccagaagagg cctcgacgac
     3481 gatagaggac caaggagagg catggacgac gatagaggtc ccagaagagg catggatgac
     3541 gacagaggtc ccagaagagg aatggacgac gacagaggac cacgcagagg actcgatgat
     3601 gatagaggtc cttggaggaa cgcagatgac gatcgcatcc cacgcagagg agctgaagat
     3661 gatagaggac cctggagaaa catggatgat gacagactca gcaggagggc tgatgatgac
     3721 aggttcccaa gaagaggaga tgattccaga cctggaccct ggaggccact cgtgaaacct
     3781 ggtggatgga gggagaaaga gaaggccaga gaagaaagct ggggtcctcc aagggagtct
     3841 aggccttctg aagagagaga atgggatagg gagaaagaga gggacaggga taaccaagat
     3901 agagaggaga acgataagga cccagaacgc gagagagaca gagagaggga tgtcgataga
     3961 gaagatcgct tccgcagacc cagggacgag ggtggatgga ggagaggtcc agctgaagaa
     4021 tcctcctcct ggagagactc ttccaggagg gacgacagag atagggatga tagaaggaga
     4081 gagagagatg acagacgcga cctcagggag cgcagggatc tcagggatga cagagacaga
     4141 agaggtcctc ctctgaggtc tgagagagag gaggtttcca gctggagaag agcagacgat
     4201 aggaaggacg atcgcgtgga ggagagagat ccacccagaa gagtccctcc accagctctg
     4261 tccagagaca gggagagaga tagggacagg gaacgcgaag gcgagaaaga gaaagcctcc
     4321 tggagagctg agaaggacag agagtctctc cgcagaacta agaacgaaac cgatgaggac
     4381 ggctggacca ccgtccgcag ggagaacctc tactttggac aggcctccga ctacaaggat
     4441 catgatggcg actacaagga tcacgacatc gactacaagg acgatgacga caaataaggt
     4501 acctctagag cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc
     4561 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4621 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4681 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4741 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     4801 gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     4861 cgctgagcaa taactatcat aacccctagg gtatacccat ctaattggaa ccagataagt
     4921 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     4981 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     5041 ccctcccagt tcccaactat tttgtccgcc cacaaccggt tgacttgggt caactgtcag
     5101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5881 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattgac
     5941 ttgggtcgct cttcctgtgg atgcgcagat gccctgcgta agcgggtgtg ggcggacaat
     6001 aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag
     6061 aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt
     6121 atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg
     6181 cgtggccaag ggcatgtaaa gactatattc gcggcgttgt gacaatttac cgaacaactc
     6241 cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     6301 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     6361 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     6421 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag
     6481 atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag
     6541 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     6601 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     6661 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     6721 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     6781 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     6841 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     6901 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     6961 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     7021 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     7081 gcagcagcga agtcgcc
//
