LOCUS       sequence-47838-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight Hygro DEST (w769-1)
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(144..149,150..167,168..174)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(294..313)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
       61 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
      121 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
      181 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      241 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      301 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      361 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      421 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
      481 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
      541 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
      601 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttt
//
