LOCUS       sequence-478380-        5705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3c for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5705)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3c-3C-STREP
FEATURES             Location/Qualifiers
     source          1..5705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2995..3018
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3019..3042
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3079..3102
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    3262..3396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3299..3318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3353..3372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3517..3682
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3872..4460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4361..4380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4595..4818
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4885..5418)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5607..5612,5613..5629,5630..5635))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgtc taggttcttc acaacaggtt ccgattctga gtctgagtcc
      301 agcctgtccg gagaggagct ggtgacaaag ccagtcggag gcaactacgg caagcaacca
      361 ctcctcctga gcgaggacga agaggatacc aagagagtcg ttaggtccgc taaagacaaa
      421 cgcttcgagg aactgactaa cctcatcaga actattcgca atgccatgaa gatcagggat
      481 gtcactaagt gtctcgaaga gttcgaactg ctgggaaagg cttacggaaa ggctaagtcc
      541 atcgtcgata aggaaggcgt tcccagattc tacatccgca tcctcgctga cctcgaagac
      601 tacctgaatg agctgtggga agataaggaa ggtaagaaga agatgaacaa gaacaacgca
      661 aaggctctgt ccactctcag gcagaagatt aggaagtaca acagggattt cgaaagccac
      721 attacttcct acaagcagaa ccctgagcaa agcgcagatg aggatgccga gaagaacgag
      781 gaagactccg aaggctcctc tgacgaggat gaggatgaag acggtgtgag cgcagcaacc
      841 ttcctgaaga agaagagcga ggcaccctcc ggagagagca gaaagtttct caagaagatg
      901 gatgacgaag acgaagactc cgaggattct gaagacgacg aggactggga tacaggtagc
      961 acttcctctg actctgacag cgaggaggag gagggaaagc agaccgcact cgcaagcaga
     1021 ttcctcaaga aggctcccac cactgacgaa gacaagaagg ctgccgagaa gaagagagag
     1081 gataaggcca agaagaagca cgatagaaag tctaagaggc tggatgaaga ggaagaagat
     1141 aacgaaggag gtgagtggga aagggtgaga ggtggtgtcc cactggtgaa agagaagcct
     1201 aagatgttcg ctaagggtac agagatcacc cacgctgttg tcatcaagaa gctgaacgaa
     1261 atcctgcagg ccagaggtaa gaagggcaca gatagagctg cccagatcga actcctgcaa
     1321 ctcctggttc agattgctgc cgagaacaac ctgggtgagg gagtgatcgt gaagatcaag
     1381 ttcaacatca ttgcttccct gtatgactac aatccaaacc tggctactta catgaagcct
     1441 gaaatgtggg gtaagtgcct cgactgcatc aacgaactga tggacattct ctttgccaac
     1501 cctaacatct tcgtgggtga gaacatcctg gaggaatccg agaacctcca caacgctgat
     1561 cagccactca gagtgagagg atgtatcctc actctggtgg aaagaatgga cgaggagttc
     1621 accaagatca tgcagaacac cgacccacac tcccaagagt atgttgaaca cctgaaggac
     1681 gaggcacagg tctgtgctat cattgaaagg gttcagcgct acctggagga gaagggcacc
     1741 actgaagagg tgtgcaggat ctacctgctc aggattctcc atacctacta caagttcgac
     1801 tacaaggcac accagaggca actgacacca cccgaaggaa gcagcaagtc cgagcaggac
     1861 caggcagaga atgaaggcga ggatagcgct gttctcatgg aacgcctgtg caagtacatc
     1921 tacgctaagg accgcactga tagaatccgc acttgcgcta tcctctgcca tatctaccat
     1981 cacgctctgc atagcagatg gtatcaggct agggatctca tgctgatgtc ccatctgcag
     2041 gacaacatcc aacatgccga ccctccagtt caaatcctct acaaccgcac tatggtccag
     2101 ctgggaatct gtgccttcag acaaggactg accaaggatg ctcacaacgc tctcctcgac
     2161 attcagtctt ctggtagggc taaggaactg ctgggacaag gactcctgct gaggagcctg
     2221 caggagagaa accaagagca ggagaaagtc gaaagaagga ggcaagttcc cttccatctc
     2281 catatcaatc tggaactgct ggagtgcgtg tacctcgtgt ccgcaatgct cctcgaaatc
     2341 ccttacatgg ctgcccacga aagcgatgca aggagacgca tgatcagcaa gcagtttcac
     2401 caccagctga gggtcggaga acgccaacca ctgctgggac cacctgaaag catgagggaa
     2461 cacgttgtgg ctgcctctaa ggctatgaag atgggtgatt ggaagacctg ccactccttc
     2521 atcatcaatg agaagatgaa cggaaaggtg tgggacctct tcccagaagc cgacaaggtt
     2581 aggaccatgc tggtccgcaa gatccaagag gagtccctca gaacttatct gttcacctat
     2641 tcttccgttt acgatagcat ctctatggaa acactcagcg atatgtttga actggatctg
     2701 ccaacagtcc attccatcat ctctaagatg atcatcaacg aggaactgat ggcctccctc
     2761 gaccagccta cacaaacagt ggtcatgcac cgcacagaac caacagccca gcagaacctc
     2821 gctctgcagc tggctgagaa gctcggctct ctcgttgaga acaacgaaag ggtgtttgac
     2881 cacaagcagg gcacttacgg aggttacttc agagatcaga aggacggcta taggaagaat
     2941 gaaggctaca tgcgcagagg tggttatcgc cagcagcaat ctcagacagc ttatctcgaa
     3001 gtcctgtttc aaggtccctg gagccatcct cagttcgaga agggctccgc tggatctgct
     3061 gctggatctg gagctggttg gagccaccct cagtttgaga agtaaggtac ctctagagcc
     3121 tgcagtctcg acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     3181 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3241 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3361 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga
     3421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     3481 actatcataa cccctagggt atacccatct aattggaacc agataagtga aatctagttc
     3541 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     3601 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     3661 ccaactattt tgtccgccca caaccggttg acttgggtca actgtcagac caagtttact
     3721 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3781 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3841 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3901 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3961 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4021 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4081 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4141 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4201 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4261 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4321 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4381 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4441 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4501 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattgactt gggtcgctct
     4561 tcctgtggat gcgcagatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac
     4621 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
     4681 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
     4741 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
     4801 catgtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     4861 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     4921 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     4981 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     5041 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     5101 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     5161 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     5221 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     5281 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     5341 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     5401 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     5461 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     5521 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     5581 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     5641 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     5701 tcgcc
//
