LOCUS       sequence-478386-        3878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3i for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3i-HIS10
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1240..1269
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     polyA_signal    1435..1569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1472..1491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1526..1545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1690..1855
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2045..2633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2534..2553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2768..2991
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3058..3591)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3780..3785,3786..3802,3803..3808))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgaa accaattctg ctgcaaggtc acgaaagatc catcactcaa
      301 atcaagtaca acagggaagg cgacctgctg ttcacagtcg ctaaagaccc aatcgtcaac
      361 gtgtggtact ccgtgaacgg tgagagactg ggcacctaca tgggtcacac tggagctgtc
      421 tggtgtgttg acgctgattg ggataccaaa catgtcctga caggtagcgc tgacaattct
      481 tgcaggctgt gggactgcga aacaggcaag cagctggctc tgctcaagac caactccgct
      541 gtgagaacct gtggctttga tttcggtggc aacatcatca tgttctctac cgacaaacaa
      601 atgggctacc agtgcttcgt ttctttcttt gatctgaggg acccttctca gatcgacaac
      661 aacgagccct acatgaagat tccctgcaac gattccaaga tcacaagcgc tgtctgggga
      721 cccctcggtg aatgtatcat tgctggccac gaaagcggag agctgaatca gtactccgca
      781 aagtctggag aagtcctggt gaacgtgaag gagcatagca gacaaatcaa tgacattcag
      841 ctctccaggg acatgactat gttcgttact gcctccaagg ataacaccgc aaagctcttc
      901 gacagcacta ccctggagca tcagaagact ttcaggacag aaagacccgt gaactccgca
      961 gccctgtctc ctaactatga tcatgtggtg ctgggaggag gacaggaggc tatggatgtg
     1021 accaccacct ctactcgcat tggaaagttc gaggctcgct tctttcatct ggcctttgaa
     1081 gaggagttcg gaagggtgaa aggtcacttt ggaccaatca actctgtggc cttccaccct
     1141 gacggaaagt cctactcttc tggtggtgaa gacggctatg ttcgcatcca ctacttcgat
     1201 ccacagtact tcgagtttga gttcgaagct ggttcctctc atcatcatca ccatcaccat
     1261 catcaccacg cctcttaagg tacctctaga gcctgcagtc tcgacaagct tgtcgagaag
     1321 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     1381 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     1441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1561 gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga
     1621 agctgagttg gctgctgcca ccgctgagca ataactatca taacccctag ggtataccca
     1681 tctaattgga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     1741 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     1801 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg
     1861 ttgacttggg tcaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2161 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2701 ctgcgttatc ccctgattga cttgggtcgc tcttcctgtg gatgcgcaga tgccctgcgt
     2761 aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca
     2821 ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
     2881 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
     2941 gcccgtcgta ttaaagaggg gcgtggccaa gggcatgtaa agactatatt cgcggcgttg
     3001 tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta
     3061 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     3121 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     3181 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     3241 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt
     3301 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     3361 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     3421 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     3481 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     3541 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc
     3601 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     3661 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     3721 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     3781 gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg
     3841 tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgcc
//
