LOCUS       sequence-478390-        4550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3l for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4550)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3l
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    2107..2241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2144..2163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2198..2217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2362..2527
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2717..3305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3206..3225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3440..3663
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3730..4263)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4452..4457,4458..4474,4475..4480))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgtc ctatcctgcc gatgactatg aatctgaggc tgcttatgat
      301 ccatacgcct acccttctga ttacgacatg cacacaggcg accctaaaca agatctggca
      361 tacgagagac aatacgaaca gcaaacctac caagtgatcc cagaggtgat caagaacttc
      421 attcaatact tccataagac cgtgtccgac ctcatcgacc agaaggtcta tgagctgcag
      481 gcaagcagag tgtcttccga cgtcattgac cagaaggtgt atgagatcca ggacatctat
      541 gagaacagct ggaccaaact gactgaaaga ttcttcaaga acactccctg gcctgaggct
      601 gaagctatcg ctccacaggt gggaaatgat gccgtgttcc tgattctgta caaggaactg
      661 tactacagac atatctacgc taaggtttct ggaggaccct ccctggaaca aagatttgag
      721 agctactaca actactgcaa tctgttcaac tacattctga atgccgatgg acctgcacct
      781 ctggaactgc ccaaccagtg gctctgggac atcattgatg agttcatcta tcagttccag
      841 tccttctctc agtacagatg taagaccgct aagaaatccg aagaggagat tgatttcctg
      901 agaagcaatc ccaagatctg gaatgttcac tctgtgctca acgtgctgca ctccctggtg
      961 gacaaatcca acatcaacag gcagctggaa gtgtacacaa gcggtggtga tcctgagagc
     1021 gttgctggtg agtacggtag acatagcctc tacaagatgc tgggctactt ctccctggtg
     1081 ggtctgctca ggctccactc tctgctcgga gactactacc aggctatcaa agtgctggag
     1141 aacatcgaac tcaacaagaa gtctatgtat agcagagtcc ctgagtgcca ggtcactact
     1201 tactactacg tgggcttcgc ctacctcatg atgagacgct accaggatgc catcagggtc
     1261 ttcgctaaca tcctcctgta catccagagg accaaatcca tgtttcaaag gaccacttac
     1321 aagtatgaaa tgatcaacaa gcagaacgaa cagatgcacg cactcctggc aattgccctg
     1381 actatgtatc ccatgagaat tgatgaatcc atccacctcc aactgagaga gaagtacgga
     1441 gataagatgc tcaggatgca gaagggtgat cctcaggttt acgaagaact gttcagctat
     1501 tcttgcccaa agtttctctc tccagtcgtc cctaactatg acaacgtgca cccaaactat
     1561 cacaaagagc ctttcctgca acagctgaaa gtgttctctg acgaggtcca gcagcaggct
     1621 caactgtcta caatccgcag cttcctgaaa ctctatacaa ctatgcctgt ggctaagctg
     1681 gcaggattcc tggatctgac tgaacaggag tttcgcattc agctgctcgt gttcaagcac
     1741 aagatgaaga acctggtgtg gacttccgga atcagcgctc tggacggtga gtttcagtct
     1801 gcaagcgagg ttgacttcta catcgacaag gacatgatcc acatcgctga tacaaaggtg
     1861 gccaggaggt acggagactt cttcatcagg cagattcaca agtttgaaga gctgaaccgc
     1921 acactcaaga agatgggaca aagaccataa ggtacctcta gagcctgcag tctcgacaag
     1981 cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
     2041 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     2101 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2161 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2221 gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg ctgctaacaa
     2281 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat cataacccct
     2341 agggtatacc catctaattg gaaccagata agtgaaatct agttccaaac tattttgtca
     2401 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     2461 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     2521 gcccacaacc ggttgacttg ggtcaactgt cagaccaagt ttactcatat atactttaga
     2581 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2641 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2701 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2761 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2821 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2881 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2941 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3001 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3061 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3361 acatgttctt tcctgcgtta tcccctgatt gacttgggtc gctcttcctg tggatgcgca
     3421 gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac aaaatagatc
     3481 taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa atcagtccag
     3541 ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc ttcattttct
     3601 gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgt aaagactata
     3661 ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg
     3721 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     3781 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
     3841 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
     3901 gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg
     3961 ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
     4021 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
     4081 ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc
     4141 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
     4201 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa
     4261 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     4321 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
     4381 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     4441 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
     4501 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgcc
//
