LOCUS       sequence-478391-        3512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3k for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3512)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3k
FEATURES             Location/Qualifiers
     source          1..3512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1069..1203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1106..1125)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1160..1179
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1324..1489
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1679..2267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2168..2187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2402..2625
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2692..3225)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3414..3419,3420..3436,3437..3442))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatggc tatgtttgag caaatgagag ccaatgttgg aaagctgctg
      301 aagggcattg acaggtacaa tcccgagaat ctggccactc tcgaacgcta tgtggaaaca
      361 caagccaagg agaatgctta cgacctggag gctaatctgg ctgtgctgaa actctaccaa
      421 ttcaaccctg cattctttca gacaaccgtg actgctcaga tcctcctgaa agccctgaca
      481 aacctgcctc acaccgactt cactctctgc aagtgcatga tcgaccaagc ccaccaagaa
      541 gaaaggccaa tcagacaaat cctctacctg ggtgatctcc tcgaaacatg ccatttccag
      601 gccttctggc aagccctgga cgagaacatg gatctcctcg aaggtatcac tggcttcgaa
      661 gatagcgtgc gcaagttcat ctgccatgtg gtgggaatca cctaccagca catcgaccgc
      721 tggctgctgg ctgaaatgct gggagatctg agcgattccc agctcaaggt gtggatgtcc
      781 aagtacggat ggtccgctga tgagagcggt cagatcttca tctgtagcca agaagagtct
      841 atcaaaccaa agaacatcgt cgagaagatt gatttcgata gcgtgtcttc catcatggct
      901 tccagccagt aaggtacctc tagagcctgc agtctcgaca agcttgtcga gaagtactag
      961 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1021 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1081 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1141 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1201 atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga
     1261 gttggctgct gccaccgctg agcaataact atcataaccc ctagggtata cccatctaat
     1321 tggaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
     1381 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
     1441 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacaa ccggttgact
     1501 tgggtcaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1561 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1621 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1681 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1741 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1801 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1861 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1921 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1981 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2041 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2101 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2161 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2221 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2281 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2341 tatcccctga ttgacttggg tcgctcttcc tgtggatgcg cagatgccct gcgtaagcgg
     2401 gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag
     2461 tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca
     2521 tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt
     2581 cgtattaaag aggggcgtgg ccaagggcat gtaaagacta tattcgcggc gttgtgacaa
     2641 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
     2701 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     2761 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     2821 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     2881 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     2941 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     3001 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     3061 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     3121 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     3181 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
     3241 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     3301 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     3361 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     3421 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     3481 acccggcaac cttgggcagc agcgaagtcg cc
//
