LOCUS       sequence-478393-        3980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3m for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3980)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3m
FEATURES             Location/Qualifiers
     source          1..3980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1537..1671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1574..1593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1628..1647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1792..1957
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2147..2735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2636..2655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2870..3093
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3160..3693)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3882..3887,3888..3904,3905..3910))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgag cgtccctgct ttcatcgaca tctctgaaga agaccaagca
      301 gccgaactga gggcttatct gaagagcaag ggagctgaaa tctctgagga gaacagcgag
      361 ggaggcctgc acgtcgatct cgctcagatc atcgaggcat gcgatgtgtg cctgaaggag
      421 gacgacaagg atgtggaaag cgttatgaat agcgttgtgt ccctcctcct gatcctcgaa
      481 cccgataagc aagaagctct catcgagtcc ctgtgcgaga agctcgtgaa gttcagggag
      541 ggagaaagac ctagcctcag gctccagctc ctctccaacc tgtttcatgg aatggacaag
      601 aacactccag tcaggtacac cgtttactgt tctctcatca aggttgctgc aagctgcggt
      661 gctatccagt acattccaac cgaactggac caagtccgca aatggatctc tgactggaac
      721 ctcactactg agaagaaaca caccctgctg aggctgctgt atgaagctct ggtggactgc
      781 aagaagagcg acgctgcctc taaagtgatg gtggagctgc tgggttccta cactgaggac
      841 aatgcttccc aggccagggt cgatgctcat aggtgcattg ttagagccct gaaggaccca
      901 aatgctttcc tgttcgacca cctcctgacc ctcaaacccg tgaagtttct ggaaggcgaa
      961 ctgatccatg atctcctgac catcttcgtg agcgctaagc tcgcctctta cgtcaagttc
     1021 taccagaaca acaaggactt catcgactcc ctgggactgc tgcatgaaca gaacatggcc
     1081 aagatgagac tgctgacctt catgggaatg gctgttgaga acaaggagat tagcttcgac
     1141 actatgcaac aggaactcca gatcggagca gatgatgtgg aggctttcgt catcgacgca
     1201 gttagaacta agatggttta ctgcaagatt gatcagactc agcgcaaagt cgtcgtgtct
     1261 cacagcactc acagaacatt cggtaagcag cagtggcaac agctgtatga cactctgaac
     1321 gcttggaagc agaacctcaa caaggtgaag aactctctcc tgagcctctc tgacacctaa
     1381 ggtacctcta gagcctgcag tctcgacaag cttgtcgaga agtactagag gatcataatc
     1441 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     1501 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
     1681 cttgagccta gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     1741 caccgctgag caataactat cataacccct agggtatacc catctaattg gaaccagata
     1801 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     1861 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     1921 cacccctccc agttcccaac tattttgtcc gcccacaacc ggttgacttg ggtcaactgt
     1981 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2041 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2101 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2161 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2221 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2281 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2341 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2401 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2461 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2521 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2581 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2641 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2701 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2761 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2821 gacttgggtc gctcttcctg tggatgcgca gatgccctgc gtaagcgggt gtgggcggac
     2881 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     2941 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     3001 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     3061 gggcgtggcc aagggcatgt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa
     3121 ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
     3181 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
     3241 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
     3301 cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
     3361 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
     3421 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
     3481 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
     3541 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
     3601 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
     3661 ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc
     3721 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
     3781 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     3841 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
     3901 cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     3961 tgggcagcag cgaagtcgcc
//
