LOCUS       sequence-478395-        8817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3b, eIF3g and eIF3i subunits for further cloning or 
            expression in insect cells for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBIG1D-eIF3bgi
FEATURES             Location/Qualifiers
     source          1..8817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(110..115,116..132,133..138))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(209..241)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(288..307)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    332..380
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        475..566
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3221..3355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3511..3602
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4775..4909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5065..5156
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             6194..6223
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     polyA_signal    6389..6523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  6837..7002
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7192..7780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7681..7700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  7915..8138
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8205..8738)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      121 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      181 ggcaaccttg ggcagcagcg aagtcgccat aacttcgtat agcatacatt atacgaagtt
      241 acctgggttt aaacctaatg atgcctgatg tttttatcga ggctaactga aacacggaag
      301 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      361 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcaa
      421 gtgaacgctc tatggtctaa agatttaaat cgacctactc cggaatatta atagatcatg
      481 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
      541 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
      601 gcggatccct cgagtcaaca tgcaagacgc tgagaacgtt gctgtgcctg aagcagcaga
      661 agagagagcc gagccaggcc agcaacaacc agctgccgag ccaccacctg ctgagggact
      721 gctgaggcct gcaggtccag gtgcacctga agctgctggt actgaggcct ctagcgaaga
      781 agtcggaatt gctgaagctg gacctgaatc tgaagtcagg accgaacctg ctgctgaggc
      841 tgaagctgct tccggtccat ccgagtctcc ctctcctcct gctgcagaag agctgcctgg
      901 ctctcatgct gaaccaccag ttccagcaca gggtgaagct cctggtgagc aggccagaga
      961 tgagaggagc gatagcaggg ctcaggctgt ctctgaagac gctggaggca acgaaggcag
     1021 ggctgccgaa gccgaaccta gagcactgga gaatggcgat gccgacgagc cttccttctc
     1081 tgacccagaa gatttcgtcg atgacgtgag cgaagaagaa ctgctgggag acgttctgaa
     1141 agatagacct caggaggctg acggcatcga ctccgtgatt gtcgttgaca acgttcctca
     1201 ggtgggtccc gacagactgg agaagctgaa gaatgtcatt cacaagatct tctctaagtt
     1261 cggcaagatc actaacgact tctatcccga ggaagatggt aagaccaagg gatacatctt
     1321 tctggagtac gcttctcctg cccatgccgt tgacgctgtc aagaacgctg acggatacaa
     1381 actcgacaag cagcatactt tcagggtcaa cctgttcacc gatttcgata agtacatgac
     1441 catttccgat gaatgggaca tcccagagaa gcagcccttc aaggatctcg gcaacctgcg
     1501 ctattggctg gaggaggctg agtgtaggga ccagtactct gttatcttcg aatccggtga
     1561 tagaacctcc atcttctgga acgatgtcaa agaccctgtc agcattgaag aaagagcaag
     1621 gtggaccgaa acttacgtga ggtggagccc taaaggcacc tatctggcca ccttccatca
     1681 gagaggcatt gccctctggg gtggtgagaa gttcaagcag atccagaggt tctcccacca
     1741 gggagtgcaa ctcatcgact tcagcccatg tgagcgctac ctcgtgacat tctctcctct
     1801 catggacaca caagacgatc ctcaggctat catcatttgg gacattctga ctggacataa
     1861 gaagagaggc ttccactgcg aatcctctgc tcattggcca atcttcaagt ggagccacga
     1921 tggcaagttc ttcgctcgca tgacactgga caccctgtcc atctacgaga caccctctat
     1981 gggtctgctg gacaagaagt ccctgaagat ctctggcatc aaggacttct cctggagccc
     2041 tggtggtaac atcattgcct tctgggtccc tgaagacaaa gacattccag ccagggtcac
     2101 actgatgcaa ctgccaacaa ggcaggagat tagagtccgc aatctgttca atgttgtgga
     2161 ctgtaaactg cactggcaga agaatggaga ctatctctgc gtgaaggtgg atagaacacc
     2221 aaagggaact caaggcgtcg tgacaaactt tgagatcttt aggatgagag agaaacaggt
     2281 gcctgttgac gtggtggaga tgaaagagac aatcatcgct ttcgcctggg agcccaacgg
     2341 ttccaagttt gccgttctgc acggagaagc tccaaggatt tccgtctctt tctaccacgt
     2401 gaagaacaac ggtaagatcg aactcatcaa gatgtttgat aagcagcagg ccaatactat
     2461 cttctggagc cctcaaggcc aattcgtggt gctcgctgga ctgaggtcta tgaacggagc
     2521 cctcgccttc gtggacacaa gcgactgtac agtgatgaac atcgcagagc actacatggc
     2581 ctctgatgtg gagtgggacc caactggaag gtacgtggtg acatctgtta gctggtggtc
     2641 ccacaaagtg gacaacgcct actggctgtg gacattccag ggtcgcctgc tccagaagaa
     2701 caacaaggac cgcttctgcc agctcctctg gaggccaaga ccacctaccc tcctgtccca
     2761 agagcaaatc aaacagatca agaaagacct caagaagtat tccaagatct tcgaacagaa
     2821 ggacagactc tctcagtcca aggcctctaa ggaactggtg gagaggagga gaactatgat
     2881 ggaagatttc cgcaagtacc gcaagatggc acaagagctg tacatggagc agaagaatga
     2941 gagactggag ctgagaggtg gtgtggatac cgacgagctg gatagcaacg ttgacgactg
     3001 ggaggaagaa accatcgagt tctttgtcac cgaagagatc atcccactcg gaaaccagga
     3061 gtaaggtacc tctagagcct gcagtctcga caagcttgtc gagaagtact agaggatcat
     3121 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3181 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc
     3361 actgcttgag cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     3421 ctgccaccgc tgagcaataa ctatcataac catttaaata aactggatac tattgcacgt
     3481 ttaaatcgac ctactccgga atattaatag atcatggaga taattaaaat gataaccatc
     3541 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
     3601 atattccgga ttattcatac cgtcccacca tcgggcgcgg atccctcgag tcaacatgcc
     3661 tacaggtgat ttcgattcca aaccctcttg ggcagatcag gtggaggaag agggagagga
     3721 tgacaaatgc gtgactagcg aactgctcaa gggcattcca ctcgccacag gcgacactag
     3781 ccctgagcca gagctgctcc caggtgcacc tctgccacca ccaaaggagg tgatcaacgg
     3841 caacatcaag acagttaccg agtacaagat tgacgaggat ggcaagaagt tcaagatcgt
     3901 tcgcaccttc aggatcgaaa ctaggaaggc atccaaagct gttgccagaa ggaagaactg
     3961 gaagaagttc ggtaactctg agttcgaccc tcctggtccc aacgtcgcta caaccacagt
     4021 gagcgacgac gtttccatga ccttcatcac aagcaaagag gacctgaatt gccaagagga
     4081 ggaagaccca atgaacaagc tcaagggaca gaagattgtc agctgtcgca tttgtaaagg
     4141 agatcactgg accacaagat gcccatacaa agatacactg ggtcccatgc agaaggagct
     4201 ggctgagcag ctgggactgt ctacaggcga gaaagagaag ctcccaggcg agctggaacc
     4261 tgtgcaggca acccagaaca agactggtaa gtacgttcca ccaagcctca gggatggtgc
     4321 ctccaggaga ggagagtcta tgcagcccaa caggagagca gatgataacg ccacaatcag
     4381 agttaccaac ctgtctgaag acacaaggga gactgatctc caagaactct ttagaccctt
     4441 cggctccatc tctcgcatct atctggccaa ggacaagaca accggacagt ccaagggctt
     4501 cgccttcatt agctttcaca ggagagagga cgctgccaga gctattgctg gtgtgtctgg
     4561 atttggctac gaccacctga tcctcaatgt cgaatgggca aagccttcca ccaactaagg
     4621 tacctctaga gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag
     4681 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     4741 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     4801 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4861 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
     4921 tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     4981 ccgctgagca ataactatca taaccattta aataaaccta atgatgcctg atgtttaaat
     5041 cgacctactc cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa
     5101 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc
     5161 cggattattc ataccgtccc accatcgggc gcggatccct cgagtcaaca tgaaaccaat
     5221 tctgctgcaa ggtcacgaaa gatccatcac tcaaatcaag tacaacaggg aaggcgacct
     5281 gctgttcaca gtcgctaaag acccaatcgt caacgtgtgg tactccgtga acggtgagag
     5341 actgggcacc tacatgggtc acactggagc tgtctggtgt gttgacgctg attgggatac
     5401 caaacatgtc ctgacaggta gcgctgacaa ttcttgcagg ctgtgggact gcgaaacagg
     5461 caagcagctg gctctgctca agaccaactc cgctgtgaga acctgtggct ttgatttcgg
     5521 tggcaacatc atcatgttct ctaccgacaa acaaatgggc taccagtgct tcgtttcttt
     5581 ctttgatctg agggaccctt ctcagatcga caacaacgag ccctacatga agattccctg
     5641 caacgattcc aagatcacaa gcgctgtctg gggacccctc ggtgaatgta tcattgctgg
     5701 ccacgaaagc ggagagctga atcagtactc cgcaaagtct ggagaagtcc tggtgaacgt
     5761 gaaggagcat agcagacaaa tcaatgacat tcagctctcc agggacatga ctatgttcgt
     5821 tactgcctcc aaggataaca ccgcaaagct cttcgacagc actaccctgg agcatcagaa
     5881 gactttcagg acagaaagac ccgtgaactc cgcagccctg tctcctaact atgatcatgt
     5941 ggtgctggga ggaggacagg aggctatgga tgtgaccacc acctctactc gcattggaaa
     6001 gttcgaggct cgcttctttc atctggcctt tgaagaggag ttcggaaggg tgaaaggtca
     6061 ctttggacca atcaactctg tggccttcca ccctgacgga aagtcctact cttctggtgg
     6121 tgaagacggc tatgttcgca tccactactt cgatccacag tacttcgagt ttgagttcga
     6181 agctggttcc tctcatcatc atcaccatca ccatcatcac cacgcctctt aaggtacctc
     6241 tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac
     6301 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6361 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     6421 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6481 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     6541 tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     6601 agcaataact atcataacca tttaaataaa tctatatctc aatcggggtt agtcgatacc
     6661 ccaccgagac cccattggga ccaatacgcc cgcgtttctt ccttttcccc accccaaccc
     6721 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcaag ccctgccata
     6781 gccactacgg gttaattaaa aacggttcac atagcttagt ttaaacagga gatccgaacc
     6841 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
     6901 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
     6961 atttccaccc ctcccagttc ccaactattt tgtccgccca caaccggttg acttgggtca
     7021 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7081 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7141 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7801 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7861 tgattgactt gggtcgctct tcctgtggat gcgcagatgc cctgcgtaag cgggtgtggg
     7921 cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa
     7981 ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga
     8041 cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta
     8101 aagaggggcg tggccaaggg catgtaaaga ctatattcgc ggcgttgtga caatttaccg
     8161 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     8221 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     8281 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     8341 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     8401 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     8461 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     8521 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     8581 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     8641 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     8701 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa
     8761 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtac
//
