LOCUS       sequence-478396-       10887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3a and eIF3c subunits for further cloning or expression in 
            insect cells for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10887)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 10887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBIG1C-eIF3ac
FEATURES             Location/Qualifiers
     source          1..10887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(110..115,116..132,133..138))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(209..242)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(288..307)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    332..380
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        475..566
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             3359..3382
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3383..3406
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3443..3466
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    3626..3760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3916..4007
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             8234..8299
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    8459..8593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8907..9072
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      9262..9850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9751..9770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  9985..10208
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(10275..10808)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      121 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      181 ggcaaccttg ggcagcagcg aagtcgccat aacttcgtat agcatacatt atacgaagtt
      241 atctgggttt aaactggata ctattgcacg tttttatcga ggctaactga aacacggaag
      301 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      361 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcaa
      421 gtgaacgctc tatggtctaa agatttaaat cgacctactc cggaatatta atagatcatg
      481 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
      541 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
      601 gcggatccct cgagtcaaca tgtctaggtt cttcacaaca ggttccgatt ctgagtctga
      661 gtccagcctg tccggagagg agctggtgac aaagccagtc ggaggcaact acggcaagca
      721 accactcctc ctgagcgagg acgaagagga taccaagaga gtcgttaggt ccgctaaaga
      781 caaacgcttc gaggaactga ctaacctcat cagaactatt cgcaatgcca tgaagatcag
      841 ggatgtcact aagtgtctcg aagagttcga actgctggga aaggcttacg gaaaggctaa
      901 gtccatcgtc gataaggaag gcgttcccag attctacatc cgcatcctcg ctgacctcga
      961 agactacctg aatgagctgt gggaagataa ggaaggtaag aagaagatga acaagaacaa
     1021 cgcaaaggct ctgtccactc tcaggcagaa gattaggaag tacaacaggg atttcgaaag
     1081 ccacattact tcctacaagc agaaccctga gcaaagcgca gatgaggatg ccgagaagaa
     1141 cgaggaagac tccgaaggct cctctgacga ggatgaggat gaagacggtg tgagcgcagc
     1201 aaccttcctg aagaagaaga gcgaggcacc ctccggagag agcagaaagt ttctcaagaa
     1261 gatggatgac gaagacgaag actccgagga ttctgaagac gacgaggact gggatacagg
     1321 tagcacttcc tctgactctg acagcgagga ggaggaggga aagcagaccg cactcgcaag
     1381 cagattcctc aagaaggctc ccaccactga cgaagacaag aaggctgccg agaagaagag
     1441 agaggataag gccaagaaga agcacgatag aaagtctaag aggctggatg aagaggaaga
     1501 agataacgaa ggaggtgagt gggaaagggt gagaggtggt gtcccactgg tgaaagagaa
     1561 gcctaagatg ttcgctaagg gtacagagat cacccacgct gttgtcatca agaagctgaa
     1621 cgaaatcctg caggccagag gtaagaaggg cacagataga gctgcccaga tcgaactcct
     1681 gcaactcctg gttcagattg ctgccgagaa caacctgggt gagggagtga tcgtgaagat
     1741 caagttcaac atcattgctt ccctgtatga ctacaatcca aacctggcta cttacatgaa
     1801 gcctgaaatg tggggtaagt gcctcgactg catcaacgaa ctgatggaca ttctctttgc
     1861 caaccctaac atcttcgtgg gtgagaacat cctggaggaa tccgagaacc tccacaacgc
     1921 tgatcagcca ctcagagtga gaggatgtat cctcactctg gtggaaagaa tggacgagga
     1981 gttcaccaag atcatgcaga acaccgaccc acactcccaa gagtatgttg aacacctgaa
     2041 ggacgaggca caggtctgtg ctatcattga aagggttcag cgctacctgg aggagaaggg
     2101 caccactgaa gaggtgtgca ggatctacct gctcaggatt ctccatacct actacaagtt
     2161 cgactacaag gcacaccaga ggcaactgac accacccgaa ggaagcagca agtccgagca
     2221 ggaccaggca gagaatgaag gcgaggatag cgctgttctc atggaacgcc tgtgcaagta
     2281 catctacgct aaggaccgca ctgatagaat ccgcacttgc gctatcctct gccatatcta
     2341 ccatcacgct ctgcatagca gatggtatca ggctagggat ctcatgctga tgtcccatct
     2401 gcaggacaac atccaacatg ccgaccctcc agttcaaatc ctctacaacc gcactatggt
     2461 ccagctggga atctgtgcct tcagacaagg actgaccaag gatgctcaca acgctctcct
     2521 cgacattcag tcttctggta gggctaagga actgctggga caaggactcc tgctgaggag
     2581 cctgcaggag agaaaccaag agcaggagaa agtcgaaaga aggaggcaag ttcccttcca
     2641 tctccatatc aatctggaac tgctggagtg cgtgtacctc gtgtccgcaa tgctcctcga
     2701 aatcccttac atggctgccc acgaaagcga tgcaaggaga cgcatgatca gcaagcagtt
     2761 tcaccaccag ctgagggtcg gagaacgcca accactgctg ggaccacctg aaagcatgag
     2821 ggaacacgtt gtggctgcct ctaaggctat gaagatgggt gattggaaga cctgccactc
     2881 cttcatcatc aatgagaaga tgaacggaaa ggtgtgggac ctcttcccag aagccgacaa
     2941 ggttaggacc atgctggtcc gcaagatcca agaggagtcc ctcagaactt atctgttcac
     3001 ctattcttcc gtttacgata gcatctctat ggaaacactc agcgatatgt ttgaactgga
     3061 tctgccaaca gtccattcca tcatctctaa gatgatcatc aacgaggaac tgatggcctc
     3121 cctcgaccag cctacacaaa cagtggtcat gcaccgcaca gaaccaacag cccagcagaa
     3181 cctcgctctg cagctggctg agaagctcgg ctctctcgtt gagaacaacg aaagggtgtt
     3241 tgaccacaag cagggcactt acggaggtta cttcagagat cagaaggacg gctataggaa
     3301 gaatgaaggc tacatgcgca gaggtggtta tcgccagcag caatctcaga cagcttatct
     3361 cgaagtcctg tttcaaggtc cctggagcca tcctcagttc gagaagggct ccgctggatc
     3421 tgctgctgga tctggagctg gttggagcca ccctcagttt gagaagtaag gtacctctag
     3481 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     3541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3721 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     3781 aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     3841 aataactatc ataaccattt aaataaactg gatactattg cacgtttaaa tcgacctact
     3901 ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag
     3961 tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt
     4021 cataccgtcc caccatcggg cgcggatccc tcgagtcaac atgccagcct actttcaaag
     4081 acctgagaac gctctgaaga gagctaatga gtttctggag gtgggcaaga agcaaccagc
     4141 actggacgtt ctctatgatg tgatgaagtc taagaagcac cgcacctggc agaagatcca
     4201 tgaaccaatc atgctgaagt acctggaact gtgcgtcgat ctgcgcaaga gccacctcgc
     4261 taaagagggt ctgtatcaat acaagaacat ctgccaacag gtgaacatca aatctctcga
     4321 agatgtcgtg agggcttatc tcaagatggc tgaggagaag accgaagctg ctaaagagga
     4381 gtcccagcag atggtcctgg acatcgagga tctcgataac attcagactc ctgagtctgt
     4441 gctgctgtct gccgtgagcg gagaagatac ccaagacagg accgataggc tgctcctcac
     4501 accctgggtg aagtttctct gggagtctta caggcagtgc ctcgacctcc tgcgcaacaa
     4561 cagcagagtg gaaagactgt accacgacat tgcccagcaa gccttcaagt tctgtctgca
     4621 atacactaga aaggcagagt tcagaaagct ctgcgataac ctcaggatgc acctgtctca
     4681 gatccagaga catcacaacc aaagcaccgc aatcaatctg aacaacccag agagccagtc
     4741 tatgcacctg gagactagac tcgtccaact ggactctgca atcagcatgg agctgtggca
     4801 ggaggctttc aaggctgtgg aggacatcca cggactgttc tccctgagca agaagccacc
     4861 caagcctcag ctgatggcaa actactacaa caaggttagc actgtgttct ggaagtccgg
     4921 aaacgctctc ttccatgctt ctaccctcca caggctgtat catctgtcca gagaaatgcg
     4981 caagaacctg acccaagatg aaatgcagag aatgagcact cgcgtgctcc tcgccaccct
     5041 gtctatccct atcactcctg agcgcaccga catcgctaga ctgctcgaca tggacggtat
     5101 catcgtggag aagcagagaa ggctggccac cctcctcggt ctgcaagctc ctcccactag
     5161 aattggcctg atcaacgaca tggtgcgctt caacgtgctc cagtatgtcg tcccagaagt
     5221 gaaggacctc tacaattggc tggaggtgga gttcaaccca ctgaagctct gcgaaagggt
     5281 cacaaaggtc ctgaactggg tgagagagca gccagagaaa gaaccagagc tgcagcagta
     5341 cgtccctcag ctccagaaca acaccatcct gaggctgctg cagcaagtgt ctcaaatcta
     5401 ccagtccatc gagtttagca ggctgacctc cctggtgcca tttgtggacg ctttccagct
     5461 cgaaagagcc attgtggacg cagctagaca ctgcgatctg caggtccgca ttgatcacac
     5521 atctaggacc ctcagcttcg gctccgacct caactacgct accagagagg atgctcccat
     5581 cggaccacac ctccaatcca tgccctccga gcagattaga aatcaactca ccgctatgtc
     5641 ctccgtcctg gcaaaggctc tggaggtcat caagcctgct catatcctcc aggagaagga
     5701 ggagcagcac cagctcgctg tcactgccta tctgaagaac tctcgcaagg agcatcagag
     5761 aatcctggca agaagacaaa ccatcgaaga gagaaaggag agactggagt ctctgaacat
     5821 ccagagggag aaagaggagc tggaacagag ggaggcagaa ctccagaaag ttcgcaaagc
     5881 cgaagaggag aggctcaggc aggaggcaaa ggagagagag aaagaaagga ttctgcaaga
     5941 acatgagcag atcaagaaga agacagttag ggagagactg gagcagatca agaagacaga
     6001 gctgggtgcc aaggctttca aggacatcga catcgaggac ctggaggaac tggacccaga
     6061 cttcatcatg gctaagcagg tcgagcaact ggagaaggag aagaaggaac tgcaggaaag
     6121 actgaagaac caagagaaga agattgatta cttcgagagg gctaaacgcc tggaggaaat
     6181 cccactgatc aagtctgctt acgaggagca gaggatcaaa gatatggatc tctgggagca
     6241 gcaggaggag gagagaatca ccaccatgca gctcgaaaga gagaaggctc tggagcacaa
     6301 gaaccgcatg agcaggatgc tcgaagatag ggacctgttc gttatgaggc tgaaggcagc
     6361 taggcagtct gtctacgagg agaaactgaa acagttcgaa gagagactgg cagaggaacg
     6421 ccataacaga ctggaggaga gaaagaggca aaggaaggag gagcgcagaa tcacctacta
     6481 cagggagaaa gaagaagagg agcaaagaag ggctgaagaa cagatgctca aggagagaga
     6541 ggagagagaa agggctgaga gagccaaacg cgaggaagag ctgagggaat accaagagag
     6601 ggttaagaag ctcgaagaag tcgagcgcaa gaagaggcaa cgcgaactgg agatcgaaga
     6661 aagagagagg aggagagagg aagagcgcag gctgggagac tcctccctga gcaggaagga
     6721 ctccagatgg ggagataggg atagcgaagg cacttggaga aagggacccg aagctgatag
     6781 cgagtggaga agaggaccac cagagaagga atggaggaga ggtgagggca gggacgagga
     6841 cagatcccat cgcagggacg aagaaaggcc acgcaggctc ggagatgacg aggataggga
     6901 gccatccctg aggccagatg acgatagagt gcccagaaga ggtatggacg atgatagagg
     6961 accaagaaga ggtcccgagg aggatcgctt ctccaggaga ggagctgatg atgataggcc
     7021 aagctggagg aatacagacg atgatagacc accacgcagg attgccgatg aggacagagg
     7081 taactggaga cacgctgatg atgataggcc tccacgcaga ggtctggatg aggatagagg
     7141 atcttggaga accgctgacg aagacagagg acctcgcaga ggtatggacg atgacagagg
     7201 acccagaaga ggaggtgcag atgacgaacg cagctcttgg aggaacgctg atgatgacag
     7261 aggtcccaga agaggcctcg acgacgatag aggaccaagg agaggcatgg acgacgatag
     7321 aggtcccaga agaggcatgg atgacgacag aggtcccaga agaggaatgg acgacgacag
     7381 aggaccacgc agaggactcg atgatgatag aggtccttgg aggaacgcag atgacgatcg
     7441 catcccacgc agaggagctg aagatgatag aggaccctgg agaaacatgg atgatgacag
     7501 actcagcagg agggctgatg atgacaggtt cccaagaaga ggagatgatt ccagacctgg
     7561 accctggagg ccactcgtga aacctggtgg atggagggag aaagagaagg ccagagaaga
     7621 aagctggggt cctccaaggg agtctaggcc ttctgaagag agagaatggg atagggagaa
     7681 agagagggac agggataacc aagatagaga ggagaacgat aaggacccag aacgcgagag
     7741 agacagagag agggatgtcg atagagaaga tcgcttccgc agacccaggg acgagggtgg
     7801 atggaggaga ggtccagctg aagaatcctc ctcctggaga gactcttcca ggagggacga
     7861 cagagatagg gatgatagaa ggagagagag agatgacaga cgcgacctca gggagcgcag
     7921 ggatctcagg gatgacagag acagaagagg tcctcctctg aggtctgaga gagaggaggt
     7981 ttccagctgg agaagagcag acgataggaa ggacgatcgc gtggaggaga gagatccacc
     8041 cagaagagtc cctccaccag ctctgtccag agacagggag agagataggg acagggaacg
     8101 cgaaggcgag aaagagaaag cctcctggag agctgagaag gacagagagt ctctccgcag
     8161 aactaagaac gaaaccgatg aggacggctg gaccaccgtc cgcagggaga acctctactt
     8221 tggacaggcc tccgactaca aggatcatga tggcgactac aaggatcacg acatcgacta
     8281 caaggacgat gacgacaaat aaggtacctc tagagcctgc agtctcgaca agcttgtcga
     8341 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     8401 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8461 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8521 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8581 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     8641 aggaagctga gttggctgct gccaccgctg agcaataact atcataacca tttaaataaa
     8701 tctatatctc aatcggggtt agtcgatacc ccaccgagac cccattggga ccaatacgcc
     8761 cgcgtttctt ccttttcccc accccaaccc ccaagttcgg gtgaaggccc agggctcgca
     8821 gccaacgtcg gggcggcaag ccctgccata gccactacgg gttaattaaa aacctaatga
     8881 tgcctgatgt ttaaacagga gatccgaacc agataagtga aatctagttc caaactattt
     8941 tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc
     9001 actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt
     9061 tgtccgccca caaccggttg acttgggtca actgtcagac caagtttact catatatact
     9121 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9181 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9241 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9301 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9361 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9421 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9481 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9541 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9601 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9661 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9721 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9781 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9841 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9901 tgctcacatg ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat
     9961 gcgcagatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat
    10021 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
    10081 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
    10141 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catgtaaaga
    10201 ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg
    10261 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
    10321 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
    10381 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
    10441 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta
    10501 cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg
    10561 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag
    10621 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga
    10681 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata
    10741 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg
    10801 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
    10861 cttggatgcc cgaggcatag actgtac
//
