LOCUS       sequence-478397-        3818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3g for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3818)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3g
FEATURES             Location/Qualifiers
     source          1..3818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1375..1509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1412..1431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1466..1485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1630..1795
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1985..2573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2474..2493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2708..2931
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2998..3531)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3720..3725,3726..3742,3743..3748))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgcc tacaggtgat ttcgattcca aaccctcttg ggcagatcag
      301 gtggaggaag agggagagga tgacaaatgc gtgactagcg aactgctcaa gggcattcca
      361 ctcgccacag gcgacactag ccctgagcca gagctgctcc caggtgcacc tctgccacca
      421 ccaaaggagg tgatcaacgg caacatcaag acagttaccg agtacaagat tgacgaggat
      481 ggcaagaagt tcaagatcgt tcgcaccttc aggatcgaaa ctaggaaggc atccaaagct
      541 gttgccagaa ggaagaactg gaagaagttc ggtaactctg agttcgaccc tcctggtccc
      601 aacgtcgcta caaccacagt gagcgacgac gtttccatga ccttcatcac aagcaaagag
      661 gacctgaatt gccaagagga ggaagaccca atgaacaagc tcaagggaca gaagattgtc
      721 agctgtcgca tttgtaaagg agatcactgg accacaagat gcccatacaa agatacactg
      781 ggtcccatgc agaaggagct ggctgagcag ctgggactgt ctacaggcga gaaagagaag
      841 ctcccaggcg agctggaacc tgtgcaggca acccagaaca agactggtaa gtacgttcca
      901 ccaagcctca gggatggtgc ctccaggaga ggagagtcta tgcagcccaa caggagagca
      961 gatgataacg ccacaatcag agttaccaac ctgtctgaag acacaaggga gactgatctc
     1021 caagaactct ttagaccctt cggctccatc tctcgcatct atctggccaa ggacaagaca
     1081 accggacagt ccaagggctt cgccttcatt agctttcaca ggagagagga cgctgccaga
     1141 gctattgctg gtgtgtctgg atttggctac gaccacctga tcctcaatgt cgaatgggca
     1201 aagccttcca ccaactaagg tacctctaga gcctgcagtc tcgacaagct tgtcgagaag
     1261 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     1321 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     1381 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1441 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1501 gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga
     1561 agctgagttg gctgctgcca ccgctgagca ataactatca taacccctag ggtataccca
     1621 tctaattgga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     1681 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     1741 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg
     1801 ttgacttggg tcaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1861 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2101 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2641 ctgcgttatc ccctgattga cttgggtcgc tcttcctgtg gatgcgcaga tgccctgcgt
     2701 aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca
     2761 ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
     2821 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
     2881 gcccgtcgta ttaaagaggg gcgtggccaa gggcatgtaa agactatatt cgcggcgttg
     2941 tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta
     3001 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     3061 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     3121 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     3181 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt
     3241 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     3301 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     3361 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     3421 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     3481 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc
     3541 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     3601 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     3661 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     3721 gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg
     3781 tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgcc
//
