LOCUS       sequence-478399-        4193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3e for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4193)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3e
FEATURES             Location/Qualifiers
     source          1..4193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1750..1884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1787..1806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1841..1860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2005..2170
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2360..2948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2849..2868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3083..3306
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3373..3906)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4095..4100,4101..4117,4118..4123))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatggc tgagtacgat ctgacaacca ggattgctca tttcctggat
      301 aggcacctgg tgtttcctct cctggagttc ctctccgtga aggagatcta caatgagaag
      361 gaactcctcc agggtaaact ggatctgctg agcgacacca acatggtcga tttcgcaatg
      421 gatgtgtaca agaacctgta cagcgacgac attcctcacg ctctcaggga gaagaggact
      481 acagttgttg ctcagctgaa gcaactgcaa gccgagactg agcccatcgt gaagatgttt
      541 gaagaccctg aaacaaccag gcagatgcag tctactagag acggacgcat gctgtttgac
      601 tatctggcag acaaacacgg cttcaggcag gagtacctcg acaccctgta caggtacgct
      661 aagttccagt acgagtgtgg caactactct ggagctgcag aatacctgta cttcttcaga
      721 gtgctggtgc cagccacaga caggaacgct ctgtcttctc tgtggggtaa gctggcttcc
      781 gaaatcctga tgcagaactg ggatgctgca atggaggacc tcaccagact caaagaaacc
      841 attgacaaca atagcgtgtc ttctccactg cagtccctcc agcaaagaac ctggctcatc
      901 cattggtccc tgttcgtgtt cttcaaccat cccaagggaa gggacaacat cattgatctg
      961 tttctgtacc aacctcaata cctgaacgcc atccagacta tgtgcccaca catcctgaga
     1021 tacctgacaa ccgctgtgat taccaacaag gacgtgagga agagaagaca ggtcctcaag
     1081 gacctggtca aggtgattca gcaggagtcc tatacataca aggacccaat cactgagttt
     1141 gtggaatgcc tgtatgtgaa cttcgacttc gacggtgctc agaagaagct gagagagtgt
     1201 gagtctgttc tcgtgaacga tttcttcctg gttgcctgcc tggaggactt catcgagaat
     1261 gccaggctct tcatcttcga aaccttctgc aggattcatc agtgcattag catcaacatg
     1321 ctggctgata agctcaacat gacacctgaa gaggctgaga gatggatcgt gaatctgatt
     1381 cgcaacgcta ggctcgacgc taagatcgac tctaagctcg gtcacgtggt gatgggcaac
     1441 aatgccgtgt ctccatacca acaggttatc gagaagacca agtccctgag ctttcgctct
     1501 cagatgctcg ccatgaacat cgagaagaag ctgaaccaga actccagatc cgaagcacct
     1561 aactgggcta cacaagatag cggtttctat taaggtacct ctagagcctg cagtctcgac
     1621 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
     1681 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     1741 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1801 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1861 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa
     1921 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc
     1981 cctagggtat acccatctaa ttggaaccag ataagtgaaa tctagttcca aactattttg
     2041 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     2101 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     2161 tccgcccaca accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt
     2221 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2281 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2941 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3001 ctcacatgtt ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc
     3061 gcagatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag
     3121 atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc
     3181 cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt
     3241 tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact
     3301 atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc
     3361 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
     3421 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
     3481 gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc
     3541 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg
     3601 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
     3661 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
     3721 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
     3781 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
     3841 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg
     3901 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     3961 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     4021 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     4081 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
     4141 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gcc
//
