LOCUS       sequence-478400-        5300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3b for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5300)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3b
FEATURES             Location/Qualifiers
     source          1..5300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    2857..2991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2894..2913)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2948..2967
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3112..3277
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3467..4055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3956..3975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4190..4413
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4480..5013)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5202..5207,5208..5224,5225..5230))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgca agacgctgag aacgttgctg tgcctgaagc agcagaagag
      301 agagccgagc caggccagca acaaccagct gccgagccac cacctgctga gggactgctg
      361 aggcctgcag gtccaggtgc acctgaagct gctggtactg aggcctctag cgaagaagtc
      421 ggaattgctg aagctggacc tgaatctgaa gtcaggaccg aacctgctgc tgaggctgaa
      481 gctgcttccg gtccatccga gtctccctct cctcctgctg cagaagagct gcctggctct
      541 catgctgaac caccagttcc agcacagggt gaagctcctg gtgagcaggc cagagatgag
      601 aggagcgata gcagggctca ggctgtctct gaagacgctg gaggcaacga aggcagggct
      661 gccgaagccg aacctagagc actggagaat ggcgatgccg acgagccttc cttctctgac
      721 ccagaagatt tcgtcgatga cgtgagcgaa gaagaactgc tgggagacgt tctgaaagat
      781 agacctcagg aggctgacgg catcgactcc gtgattgtcg ttgacaacgt tcctcaggtg
      841 ggtcccgaca gactggagaa gctgaagaat gtcattcaca agatcttctc taagttcggc
      901 aagatcacta acgacttcta tcccgaggaa gatggtaaga ccaagggata catctttctg
      961 gagtacgctt ctcctgccca tgccgttgac gctgtcaaga acgctgacgg atacaaactc
     1021 gacaagcagc atactttcag ggtcaacctg ttcaccgatt tcgataagta catgaccatt
     1081 tccgatgaat gggacatccc agagaagcag cccttcaagg atctcggcaa cctgcgctat
     1141 tggctggagg aggctgagtg tagggaccag tactctgtta tcttcgaatc cggtgataga
     1201 acctccatct tctggaacga tgtcaaagac cctgtcagca ttgaagaaag agcaaggtgg
     1261 accgaaactt acgtgaggtg gagccctaaa ggcacctatc tggccacctt ccatcagaga
     1321 ggcattgccc tctggggtgg tgagaagttc aagcagatcc agaggttctc ccaccaggga
     1381 gtgcaactca tcgacttcag cccatgtgag cgctacctcg tgacattctc tcctctcatg
     1441 gacacacaag acgatcctca ggctatcatc atttgggaca ttctgactgg acataagaag
     1501 agaggcttcc actgcgaatc ctctgctcat tggccaatct tcaagtggag ccacgatggc
     1561 aagttcttcg ctcgcatgac actggacacc ctgtccatct acgagacacc ctctatgggt
     1621 ctgctggaca agaagtccct gaagatctct ggcatcaagg acttctcctg gagccctggt
     1681 ggtaacatca ttgccttctg ggtccctgaa gacaaagaca ttccagccag ggtcacactg
     1741 atgcaactgc caacaaggca ggagattaga gtccgcaatc tgttcaatgt tgtggactgt
     1801 aaactgcact ggcagaagaa tggagactat ctctgcgtga aggtggatag aacaccaaag
     1861 ggaactcaag gcgtcgtgac aaactttgag atctttagga tgagagagaa acaggtgcct
     1921 gttgacgtgg tggagatgaa agagacaatc atcgctttcg cctgggagcc caacggttcc
     1981 aagtttgccg ttctgcacgg agaagctcca aggatttccg tctctttcta ccacgtgaag
     2041 aacaacggta agatcgaact catcaagatg tttgataagc agcaggccaa tactatcttc
     2101 tggagccctc aaggccaatt cgtggtgctc gctggactga ggtctatgaa cggagccctc
     2161 gccttcgtgg acacaagcga ctgtacagtg atgaacatcg cagagcacta catggcctct
     2221 gatgtggagt gggacccaac tggaaggtac gtggtgacat ctgttagctg gtggtcccac
     2281 aaagtggaca acgcctactg gctgtggaca ttccagggtc gcctgctcca gaagaacaac
     2341 aaggaccgct tctgccagct cctctggagg ccaagaccac ctaccctcct gtcccaagag
     2401 caaatcaaac agatcaagaa agacctcaag aagtattcca agatcttcga acagaaggac
     2461 agactctctc agtccaaggc ctctaaggaa ctggtggaga ggaggagaac tatgatggaa
     2521 gatttccgca agtaccgcaa gatggcacaa gagctgtaca tggagcagaa gaatgagaga
     2581 ctggagctga gaggtggtgt ggataccgac gagctggata gcaacgttga cgactgggag
     2641 gaagaaacca tcgagttctt tgtcaccgaa gagatcatcc cactcggaaa ccaggagtaa
     2701 ggtacctcta gagcctgcag tctcgacaag cttgtcgaga agtactagag gatcataatc
     2761 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     2821 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     2881 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2941 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
     3001 cttgagccta gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     3061 caccgctgag caataactat cataacccct agggtatacc catctaattg gaaccagata
     3121 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     3181 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     3241 cacccctccc agttcccaac tattttgtcc gcccacaacc ggttgacttg ggtcaactgt
     3301 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3361 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3421 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3481 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3541 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3601 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3661 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3721 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3781 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3841 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4081 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4141 gacttgggtc gctcttcctg tggatgcgca gatgccctgc gtaagcgggt gtgggcggac
     4201 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     4261 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     4321 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     4381 gggcgtggcc aagggcatgt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa
     4441 ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
     4501 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
     4561 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
     4621 cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
     4681 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
     4741 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
     4801 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
     4861 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
     4921 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
     4981 ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc
     5041 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
     5101 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     5161 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
     5221 cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     5281 tgggcagcag cgaagtcgcc
//
