LOCUS       sequence-478403-        3929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3f for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3929)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3f
FEATURES             Location/Qualifiers
     source          1..3929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1486..1620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1523..1542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1577..1596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1741..1906
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2096..2684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2585..2604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2819..3042
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3109..3642)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3831..3836,3837..3853,3854..3859))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatggc aacaccagcc gttcctgtgt ctgctccacc agctactcca
      301 actcccgttc ctgcagcagc tccagccagc gttccagctc ctactcctgc tcctgctgca
      361 gctcccgtcc ctgctgctgc acctgcaagc tcttccgacc ctgctgctgc tgctgctgca
      421 actgctgcac caggtcagac accagcttcc gctcaggctc ctgcacagac accagctcca
      481 gcactgcctg gaccagctct gccaggacct ttcccaggag gaagggtggt tcgcctgcat
      541 cctgttatcc tggcttccat tgtcgatagc tacgagagaa gaaacgaagg tgctgctagg
      601 gtgattggta ctctcctcgg tacagttgac aagcattctg tcgaggtgac taactgtttc
      661 tctgtgccac acaatgaaag cgaagacgag gttgccgtgg acatggagtt cgcaaagaac
      721 atgtacgaac tgcataagaa agttagccct aatgaactga tcctcggttg gtacgccact
      781 ggacacgaca tcactgagca ctccgtgctc attcatgaat actactccag agaggctcca
      841 aatcctatcc acctgacagt ggacacctcc ctgcagaacg gtcgcatgag catcaaggcc
      901 tacgtgtcta cactgatggg agttccaggc aggacaatgg gtgtgatgtt cactccactg
      961 acagtgaagt acgcttacta tgacaccgaa aggattggcg tggacctgat catgaagact
     1021 tgcttcagcc caaacagagt catcggactc tcctctgatc tgcagcaagt tggtggagcc
     1081 tctgccagaa tccaggacgc actctctacc gtcctgcagt atgccgagga tgtgctgtcc
     1141 ggtaaggtgt ccgcagataa cactgtggga cgcttcctca tgtccctggt taaccaagtc
     1201 ccaaagatcg ttccagacga cttcgaaacc atgctgaact ccaacatcaa cgacctgctc
     1261 atggttactt acctcgccaa tctcactcaa tcccagatcg cactgaacga gaagctggtc
     1321 aatctctaag gtacctctag agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg
     1381 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1441 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     1621 tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     1681 ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc atctaattgg
     1741 aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct
     1801 tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa
     1861 ctccatttcc acccctccca gttcccaact attttgtccg cccacaaccg gttgacttgg
     1921 gtcaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1981 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2041 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2101 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2161 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2221 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2281 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2341 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2401 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2461 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2521 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2581 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2641 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2701 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2761 cccctgattg acttgggtcg ctcttcctgt ggatgcgcag atgccctgcg taagcgggtg
     2821 tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct
     2881 taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac
     2941 tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt
     3001 attaaagagg ggcgtggcca agggcatgta aagactatat tcgcggcgtt gtgacaattt
     3061 accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt
     3121 acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc
     3181 cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt
     3241 ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc
     3301 ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac
     3361 gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt
     3421 cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc
     3481 tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc
     3541 agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg
     3601 gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca
     3661 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
     3721 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     3781 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa
     3841 ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     3901 cggcaacctt gggcagcagc gaagtcgcc
//
