LOCUS       sequence-478404-        3743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3j for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3743)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-STREP-3C-eIF3j
FEATURES             Location/Qualifiers
     source          1..3743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             259..282
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             319..342
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             343..366
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    1300..1434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1337..1356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1391..1410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1555..1720
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1910..2498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2399..2418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2633..2856
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2923..3456)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3645..3650,3651..3667,3668..3673))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgtg gtcccatcct caattcgaga agggttctgc tggatctgct
      301 gctggaagcg gagcaggctg gtcccatcct caattcgaga aactggaggt gctgttccag
      361 ggtcctatgg ctgctgctgc agctgcagca ggcgatagcg actcctggga tgccgacgcc
      421 ttctccgtgg aagaccctgt ccgcaaagtg ggaggaggag gcactgctgg tggcgatcgc
      481 tgggaaggtg aggacgagga cgaagacgtg aaagataact gggatgatga cgatgatgag
      541 aagaaggagg aagccgaggt gaaacctgag gtgaagatca gcgagaagaa gaagatcgct
      601 gagaagatca aggagaaaga gaggcagcag aagaagaggc aggaagagat caagaagaga
      661 ctggaagagc ctgaggaacc caaggtgctc actccagagg agcagctggc tgacaagctg
      721 agactcaaga agctccagga agaaagcgac ctggagctgg ccaaggaaac cttcggtgtc
      781 aacaatgccg tgtacggaat cgacgccatg aatccctctt ccagagacga tttcacagag
      841 ttcggtaagc tcctgaagga taagattaca caatacgaga agtctctcta ctacgcctcc
      901 tttctcgaag tgctggtgag agatgtgtgc atttctctgg aaatcgacga cctgaagaag
      961 atcactaact ccctgactgt cctctgctcc gagaagcaga agcaagagaa acaaagcaag
     1021 gccaagaaga agaagaaggg agtggtgcca ggtggtggac tgaaggcaac catgaaggat
     1081 gacctcgcag attacggtgg ttacgacggt ggctacgttc aggactatga ggacttcatg
     1141 taaggtacct ctagagcctg cagtctcgac aagcttgtcg agaagtacta gaggatcata
     1201 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     1261 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     1321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     1441 ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     1501 tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa ttggaaccag
     1561 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     1621 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     1681 ttccacccct cccagttccc aactattttg tccgcccaca accggttgac ttgggtcaac
     1741 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1801 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1861 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1921 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1981 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2041 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2101 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2161 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2221 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2281 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2341 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2401 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2461 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2521 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2581 attgacttgg gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg ggtgtgggcg
     2641 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     2701 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     2761 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     2821 gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca atttaccgaa
     2881 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     2941 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     3001 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     3061 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     3121 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     3181 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     3241 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     3301 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     3361 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     3421 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     3481 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     3541 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     3601 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     3661 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     3721 ccttgggcag cagcgaagtc gcc
//
