LOCUS       sequence-478412-       10151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SARS-CoV-2 Protease 3CLpro expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Edri S, El-Atawneh S, Ernst T, Elnekave M, Katzman C, Lanton T, 
            Aldar I, Wolk O, Stern N, Goldblum A, Nissim L
  TITLE     Optimized pipeline and designer cells for synthetic-biology-based 
            high-throughput screening of viral protease inhibitors.
  JOURNAL   Cell Rep Methods. 2025 Aug 5:101139. doi: 
            10.1016/j.crmeth.2025.101139.
  PUBMED    40780218
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods. 2025 Aug 5:101139. doi: 10.1016/j.crmeth.2025.101139."
COMMENT     Sequence Label: pSE152: pFuGW-hUbCp-3CLpro-SV40NLS
FEATURES             Location/Qualifiers
     source          1..10151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             99..143
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    441..558
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        623..1834
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1671..1690
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2796..2816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2844..3432
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2897..2917)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3315..3326)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3435..3451)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3436..3452)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3957..4137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4163..4180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4169..4393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4439..4867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4526..4545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4736..4757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4876..4896)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4881..5210
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5061..5196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5123..5142
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5258..5305
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5324..5698
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5828..5949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5865..5884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5919..5938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5998..6014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5998..6014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6011..6033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6022..6038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6046..6052,6053..6070,6071..6076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6091..6112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6229..6246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6400..6988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6480..6499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7159..7950,7951..8019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7782..7801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8020..8124)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8199..8218)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8390..8769
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8771..8969
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8924..8944
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8987..9167
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9214..9339
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9832..10065
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
       61 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
      121 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
      181 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
      241 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
      301 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
      361 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
      421 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
      481 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
      541 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
      601 aacccgtgtc ggctccagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
      661 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
      721 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
      781 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
      841 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
      901 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
      961 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     1021 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     1081 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     1141 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
     1201 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
     1261 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     1321 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
     1381 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
     1441 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     1501 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     1561 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     1621 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     1681 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     1741 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
     1801 taaattctgg ccgtttttgg cttttttgtt agacgaagct tgggctgcag gtcgactcta
     1861 gaggatccgc caccatgagt ggttttagaa aaatggcatt ccccagcggt aaagttgaag
     1921 gatgtatggt ccaagtaacc tgtggtacca ctacccttaa tgggctttgg ttggacgacg
     1981 tagtctactg cccccgacac gtaatctgca ctagtgagga tatgcttaat cccaattacg
     2041 aagacctttt gattcggaaa tccaatcaca acttcctggt ccaagcgggc aacgtccaac
     2101 tcagggttat tggacatagt atgcagaatt gcgtactgaa gctcaaagtc gatactgcaa
     2161 accccaagac gcccaagtat aaattcgtcc gaatccaacc aggccaaaca ttttccgtat
     2221 tggcttgcta taatggaagc cccagcggtg tctaccaatg tgcaatgaga ccaaacttta
     2281 cgataaaggg ttcatttctg aacggctctt gcggttccgt tggttttaac atcgactatg
     2341 actgtgtatc cttttgctac atgcaccata tggaactccc taccggtgtc cacgccggta
     2401 cagatctgga aggaaatttc tacggtccgt tcgttgaccg gcaaaccgcg caagcggctg
     2461 gaaccgacac aacgattaca gtgaatgtgc tcgcgtggct gtacgcagca gtcataaacg
     2521 gagacaggtg gtttctgaac cgatttacga cgactctcaa tgacttcaac cttgttgcga
     2581 tgaagtacaa ttacgagcca ctcacccagg accatgttga tatcctgggt cccctcagtg
     2641 cccagacagg gatcgcagtt ctcgatatgt gcgcgtcact gaaggagctt ctccaaaatg
     2701 gaatgaatgg gcggaccata cttggttccg cactcctcga agatgaattt actccatttg
     2761 acgtggtcag acaatgctca ggtgttactt tccaaccaaa gaagaagcgg aaggtctagg
     2821 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     2881 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2941 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3001 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3061 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3121 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3181 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3241 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3301 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3361 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3421 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     3481 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     3541 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     3601 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3661 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     3721 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     3781 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     3841 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     3901 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     3961 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4021 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4081 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     4141 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4201 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4261 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4321 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4381 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4981 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5041 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5101 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5161 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5221 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     5281 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     5341 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     5401 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     5461 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     5521 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     5581 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     5641 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     5701 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5761 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5821 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5881 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5941 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6001 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6061 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6121 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6181 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     8161 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     8221 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     8281 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     8341 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     8401 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     8461 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     8521 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     8581 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     8641 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     8701 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     8761 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     8821 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     8881 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     8941 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     9001 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9061 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9121 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     9181 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9241 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     9301 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     9361 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     9421 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9481 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9541 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9601 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9661 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9721 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9781 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9841 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9901 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9961 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    10021 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    10081 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    10141 tctggaacag a
//
