LOCUS       sequence-478439-        9331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for biosynthetic pathway, pcepA-phiC31 integrase, 
            altered to be compatible with inducible landing pads generated in 
            this study.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Nakamura AK, Fulk EM, Johnson CW, Isaacs FJ
  TITLE     Synthetic Genetic Elements Enable Rapid Characterization of 
            Inorganic Carbon Uptake Systems in Cupriavidus necator H16.
  JOURNAL   ACS Synth Biol. 2025 Mar 21;14(3):943-953. doi: 
            10.1021/acssynbio.4c00869. Epub 2025 Mar 6.
  PUBMED    40048245
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00869"; journalName: "ACS Synth Biol"; date: 
            "2025-03-21- 21"; volume: "14"; issue: "3"; pages: "943-953"
COMMENT     Sequence Label: pPath-pcepA-integrase vector-v2
FEATURES             Location/Qualifiers
     source          1..9331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            150..258
                     /note="incP origin of transfer"
     rep_origin      277..665
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             2463..4280
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     promoter        complement(4281..4299)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4282..4299)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    4302..4335
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        4369..4814
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(4815..4901,4902..6236)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        6577..6920
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6951..7760
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7018..7037)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7766..7963
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             8100..8117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8170..8188)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8184..8231
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        8339..8430
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8431..8499,8500..9291)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      join(9295..9331,1..3)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ggacgaccct tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
       61 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
      121 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
      181 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
      241 gctctgcgag gctggccgta ggccggccgg cgcgccgatc tgaagatcag cagttcaacc
      301 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac
      361 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta
      421 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
      481 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
      541 taaggctttt aaagccttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
      601 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc
      661 tgacatgggg cgcgcccagg tcagtcacag tggagcctag cactcactca gcgtgacggc
      721 tcagagcaga attcacgagc cagaaatagt aacttttgcc taaatcacaa attgcaaaat
      781 ttaattgctt gcaaaaggtc acatgcttat aatcaacttt tttaaaaatt taaaatactt
      841 ttttattttt tatttttaaa cataaatgaa ataatttatt tattgtttat gattaccgaa
      901 acataaaacc tgctcaagaa aaagaaactg ttttgtcctt ggaaaaaaag cactacctag
      961 gagcggccaa aatgccggct tacattttat gttagctggt ggactgacgc cagaaaatgt
     1021 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac
     1081 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt
     1141 tattactgag tagtatttat ttaagtattg tttgtgcact tgcctgcaag ccttttgaaa
     1201 agcaagcata aaagatctaa acataaaatc tgtaaaataa caagatgtaa agataatgct
     1261 aaatcatttg gctttttgat tgattgtaca ggaaaatata catcgcaggg ggttgacttt
     1321 taccatttca ccgcaatgga attaaacttg ttgaagagaa tgttcacagg cgcatacgct
     1381 acaatgaccc gattcttgct agccgaattc cagtcaggct gctagcacca gagctacgtg
     1441 accgcaggac tagctccagc tgagcgacag cggcaagaaa gccatccacg ccgaaaaccc
     1501 cgcttcggcg gggttttgcc gcatcttgcc gtaactgaca aattaaccga aggtttcttc
     1561 cggccactgg gatgcaatga ccttaccaac aacgctacag gattcgttgc acgggatcat
     1621 tgggtattgc ggattcagcg gctgcaggaa aacttggcca gagtcgcgga tcagcttctt
     1681 aaaggtgaat tcatcaccac ccaggcgcgc aatgcaaaag tctcccggct caaccgcctg
     1741 ctcaggatca accaggatca gcataccatc cggaaatgac ggcttgctgc ccgtcggggc
     1801 cgtcatgctg ttgccttcta cttccagcca aaacgcgcta tcgctcgctt ttttggtagt
     1861 cgatacccaa cgctcagcgt cacctttggt gaaggtacgc agttccgggc taaacatacc
     1921 tgcttgaacg tggctgaaga ccgggtattc gtattcagag cgcagagacg gttgcatgct
     1981 aaccgcttca tacatttcat agatttcacg agctatagaa ggagagaact cctcgacgga
     2041 aactttcaga atcttggtca gcagtgcggc gttgtacgcg ttcagcgcat taataccgtt
     2101 aaacagagcg ccaacgccgc tctgacccat gcccatcttg tcggctacgc tttcctgaga
     2161 cagacccagc tcattctttt tcttctcata gattgctttc agacgacgtg cgtcctctag
     2221 ctgctcttgt gtaagcggct ttttcttcgt tgacattttg atcctccttt atatggaggt
     2281 actaaatcgg aacgttaaat ctatcaccgc aagggataaa tatctaacac cgtgcgtgtt
     2341 gactatttta cctctggcgg tgataatggt tgcatcaaat ttgcgcgcca cattattatt
     2401 catacctttg tggaccgtat tacaaagaga agtgttaaat caaacaaaaa ggaggattaa
     2461 tcatggacac gtacgcgggt gcttacgacc gtcagtcgcg cgagcgcgaa aattcgagcg
     2521 cagcaagccc agcgacacag cgtagcgcca acgaagacaa ggcggccgac cttcagcgcg
     2581 aagtcgagcg cgacgggggc cggttcaggt tcgtcgggca tttcagcgaa gcgccgggca
     2641 cgtcggcgtt cgggacggcg gagcgcccgg agttcgaacg catcctgaac gaatgccgcg
     2701 ccgggcggct caacatgatc attgtctatg acgtgtcgcg cttctcgcgc ctgaaggtca
     2761 tggacgcgat tccgattgtc tcggaattgc tcgccctggg cgtgacgatt gtttccactc
     2821 aggaaggcgt cttccggcag ggaaacgtca tggacctgat tcacctgatt atgcggctcg
     2881 acgcgtcgca caaagaatct tcgctgaagt cggcgaagat tctcgacacg aagaaccttc
     2941 agcgcgaatt gggcgggtac gtcggcggga aggcgcctta cggcttcgag cttgtttcgg
     3001 agacgaagga gatcacgcgc aacggccgaa tggtcaatgt cgtcatcaac aagcttgcgc
     3061 actcgaccac tccccttacc ggacccttcg agttcgagcc cgacgtaatc cggtggtggt
     3121 ggcgtgagat caagacgcac aaacaccttc ccttcaagcc gggcagtcaa gccgccattc
     3181 acccgggcag catcacgggg ctttgtaagc gcatggacgc tgacgccgtg ccgacccggg
     3241 gcgagacgat tgggaagaag accgcttcaa gcgcctggga cccggcaacc gttatgcgaa
     3301 tccttcggga cccgcgtatt gcgggcttcg ccgctgaggt gatctacaag aagaagccgg
     3361 acggcacgcc gaccacgaag attgagggtt accgcattca gcgcgacccg atcacgctcc
     3421 ggccggtcga gcttgattgc ggaccgatca tcgagcccgc tgagtggtat gagcttcagg
     3481 cgtggttgga cggcaggggg cgcggcaagg ggctttcccg ggggcaagcc attctgtccg
     3541 ccatggacaa gctgtactgc gagtgtggcg ccgtcatgac ttcgaagcgc ggggaagaat
     3601 cgatcaagga ctcttaccgc tgccgtcgcc ggaaggtggt cgacccgtcc gcacctgggc
     3661 agcacgaagg cacgtgcaac gtcagcatgg cggcactcga caagttcgtt gcggaacgca
     3721 tcttcaacaa gatcaggcac gccgaaggcg acgaagagac gttggcgctt ctgtgggaag
     3781 ccgcccgacg cttcggcaag ctcactgagg cgcctgagaa gagcggcgaa cgggcgaacc
     3841 ttgttgcgga gcgcgccgac gccctgaacg cccttgaaga gctgtacgaa gaccgcgcgg
     3901 caggcgcgta cgacggaccc gttggcagga agcacttccg gaagcaacag gcagcgctga
     3961 cgctccggca gcaaggggcg gaagagcggc ttgccgaact tgaagccgcc gaagccccga
     4021 agcttcccct tgaccaatgg ttccccgaag acgccgacgc tgacccgacc ggccctaagt
     4081 cgtggtgggg gcgcgcgtca gtagacgaca agcgcgtgtt cgtcgggctc ttcgtagaca
     4141 agatcgttgt cacgaagtcg actacgggca gggggcaggg aacgcccatc gagaagcgcg
     4201 cttcgatcac gtgggcgaag ccgccgaccg acgacgacga agacgacgcc caggacggca
     4261 cggaagacgt agcggcgtaa tctatagtgt cacctaaatg ggtgccaggg cgtgcccgtg
     4321 ggctccccgg gcgcgtatag ggataacagg gtaatacact gagacctcca catatacctg
     4381 ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa
     4441 ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga
     4501 tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac
     4561 aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taatagaatg aggtcgaaaa
     4621 gtaaatcgcg cgggtttgtt actgataaag caggcaagac ctaaaatgtg taaagggcaa
     4681 agtgtatact ttggcgtcac cccttacata ttttaggtct ttttttattg tgcgtaacta
     4741 acttgccatc ttcaaacagg agggctggaa gaagcagacc gctaacacag tacataaaaa
     4801 aggagacatg aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta
     4861 ctaccgcact gctggcagga ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc
     4921 catataagga aacatacggc atttcacata ttacacgcca tgatatgctg caaatccctg
     4981 aacagcaaaa aaatgaaaaa tatcaagttc ctgagttcga ttcgtccaca attaaaaata
     5041 tctcttctgc aaaaggcctg gacgtttggg acagctggcc attacaaaac gctgacggca
     5101 ctgtcgcaaa ctatcacggc taccacatcg tctttgcatt agccggagat cctaaaaatg
     5161 cggatgacac atcgatttac atgttctatc aaaaagtcgg cgaaacttct attgacagct
     5221 ggaaaaacgc tggccgcgtc tttaaagaca gcgacaaatt cgatgcaaat gattctatcc
     5281 taaaagacca aacacaagaa tggtcaggtt cagccacatt tacatctgac ggaaaaatcc
     5341 gtttattcta cactgatttc tccggtaaac attacggcaa acaaacactg acaactgcac
     5401 aagttaacgt atcagcatca gacagctctt tgaacatcaa cggtgtagag gattataaat
     5461 caatctttga cggtgacgga aaaacgtatc aaaatgtaca gcagttcatc gatgaaggca
     5521 actacagctc aggcgacaac catacgctga gagatcctca ctacgtagaa gataaaggcc
     5581 acaaatactt agtatttgaa gcaaacactg gaactgaaga tggctaccaa ggcgaagaat
     5641 ctttatttaa caaagcatac tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa
     5701 aacttctgca aagcgataaa aaacgcacgg ctgagttagc aaacggcgct ctcggtatga
     5761 ttgagctaaa cgatgattac acactgaaaa aagtgatgaa accgctgatt gcatctaaca
     5821 cagtaacaga tgaaattgaa cgcgcgaacg tctttaaaat gaacggcaaa tggtacctgt
     5881 tcactgactc ccgcggatca aaaatgacga ttgacggcat tacgtctaac gatatttaca
     5941 tgcttggtta tgtttctaat tctttaactg gcccatacaa gccgctgaac aaaactggcc
     6001 ttgtgttaaa aatggatctt gatcctaacg atgtaacctt tacttactca cacttcgctg
     6061 tacctcaagc gaaaggaaac aatgtcgtga ttacaagcta tatgacaaac agaggattct
     6121 acgcagacaa acaatcaacg tttgcgccaa gtttcctgct gaacatcaaa ggcaagaaaa
     6181 catctgttgt caaagacagc atccttgaac aaggacaatt aacagttaac aaataaggtc
     6241 tctagagcca ccacagtggt taattaaggc ctgtgaggcc ggaccaaaac gaaaaaaggc
     6301 ccccctttcg ggaggcctct tttctggaat ttggtaccga gacgtcaaat tctatcataa
     6361 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     6421 tgaaaaggct gttttctgta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     6481 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaag aaattgcata
     6541 cctttgttcc tcggttatat gtttgctcat ctgcaagaca tggaggccca gaataccctc
     6601 cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag
     6661 cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga
     6721 agcaaaaatt acggcacctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac
     6781 gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca
     6841 ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc
     6901 acatccgaac ataaacaacc aaaaatttgt aattaagaag gagtgattac atgggtaagg
     6961 aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     7021 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     7081 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     7141 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     7201 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag
     7261 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     7321 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     7381 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gtgagtgatt
     7441 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
     7501 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     7561 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     7621 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     7681 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     7741 tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt
     7801 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     7861 ttatattttt tttcgcctcg acatcatctg tccagatgcg aagttaagtg cgcagaaagt
     7921 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctctagc ataaccccgc
     7981 ggggcctctt tcggggatct cgcggggttt tttgctgaaa gaagcttcaa ataaaacgaa
     8041 aggctcagtc gaaagactgg gcctttcgtt atgttgttgt cgctgcggcc gcactcgagc
     8101 accaccacca ccaccactgg gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     8161 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     8221 ggggtttttt gctgaagtac cgaggggtgc cagggcgtgc cccagggctc cccgggcgcg
     8281 tataatcgat ttaaattagt agcccgccta atgagcgggc ttttttttaa ttcccctatt
     8341 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8401 atgcttcaat aatattgaaa aaggaagagt atgagcattc agcattttcg tgtggcgctg
     8461 attccgtttt ttgcggcgtt ttgcctgccg gtgtttgcgc atccggaaac cctggtgaaa
     8521 gtgaaagatg cggaagatca actgggtgcg cgcgtgggct atattgaact ggatctgaac
     8581 agcggcaaaa ttctggaatc ttttcgtccg gaagaacgtt ttccgatgat gagcaccttt
     8641 aaagtgctgc tgtgcggtgc ggttctgagc cgtgtggatg cgggccagga acaactgggc
     8701 cgtcgtattc attatagcca gaacgatctg gtggaatata gcccggtgac cgaaaaacat
     8761 ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg cgattaccat gagcgataac
     8821 accgcggcga acctgctgct gacgaccatt ggcggtccga aagaactgac cgcgtttctg
     8881 cataacatgg gcgatcatgt gacccgtctg gatcgttggg aaccggaact gaacgaagcg
     8941 attccgaacg atgaacgtga taccaccatg ccggcagcaa tggcgaccac cctgcgtaaa
     9001 ctgctgacgg gtgagctgct gaccctggca agccgccagc aactgattga ttggatggaa
     9061 gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc cggctggctg gtttattgcg
     9121 gataaaagcg gtgcgggcga acgtggcagc cgtggcatta ttgcggcgct gggcccggat
     9181 ggtaaaccga gccgtattgt ggtgatttat accaccggca gccaggcgac gatggatgaa
     9241 cgtaaccgtc agattgcgga aattggcgcg agcctgatta aacattggta aaccgataca
     9301 attaaaggct ccttttggag cctttttttt t
//
