LOCUS       sequence-478501-       11875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NanoLuc-EP300 Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoLuc-EP300 Fusion Vector
FEATURES             Location/Qualifiers
     source          1..11875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1577
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             4740..6590
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      6354..6363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8954..8972)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    9009..9130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9020..9039)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9074..9093
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9375..9732
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9375..9566
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9398..9418)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9583..9718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9747..9794
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9826..10620
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9880..9899)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10490..10509
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10684..10732
                     /note="synthetic polyadenylation signal"
     rep_origin      10889..11434
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     11335..11354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11551..11568
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggccgagaat gtggtggaac
     1621 cggggccgcc ttcagccaag cggcctaaac tctcatctcc ggccctctcg gcgtccgcca
     1681 gcgatggcac agattttggc tctctatttg acttggagca cgacttacca gatgaattaa
     1741 tcaactctac agaattggga ctaaccaatg gtggtgatat taatcagctt cagacaagtc
     1801 ttggcatggt acaagatgca gcttctaaac ataaacagct gtcagaattg ctgcgatctg
     1861 gtagttcccc taacctcaat atgggagttg gtggcccagg tcaagtcatg gccagccagg
     1921 cccaacagag cagtcctgga ttaggtttga taaatagcat ggtcaaaagc ccaatgacac
     1981 aggcaggctt gacttctccc aacatgggga tgggcactag tggaccaaat cagggtccta
     2041 cgcagtcaac aggtatgatg aacagtccag taaatcagcc tgccatggga atgaacacag
     2101 ggatgaatgc gggcatgaat cctggaatgt tggctgcagg caatggacaa gggataatgc
     2161 ctaatcaagt catgaacggt tcaattggag caggccgagg gcgacagaat atgcagtacc
     2221 caaacccagg catgggaagt gctggcaact tactgactga gcctcttcag cagggctctc
     2281 cccagatggg aggacaaaca ggattgagag gcccccagcc tcttaagatg ggaatgatga
     2341 acaaccccaa tccttatggt tcaccatata ctcagaatcc tggacagcag attggagcca
     2401 gtggccttgg tctccagatt cagacaaaaa ctgtactatc aaataactta tctccatttg
     2461 ctatggacaa aaaggcagtt cctggtggag gaatgcccaa catgggtcaa cagccagccc
     2521 cgcaggtcca gcagccaggc ctggtgactc cagttgccca agggatgggt tctggagcac
     2581 atacagctga tccagagaag cgcaagctca tccagcagca gcttgttctc cttttgcatg
     2641 ctcacaagtg ccagcgccgg gaacaggcca atggggaagt gaggcagtgc aaccttcccc
     2701 actgtcgcac aatgaagaat gtcctaaacc acatgacaca ctgccagtca ggcaagtctt
     2761 gccaagtggc acactgtgca tcttctcgac aaatcatttc acactggaag aattgtacaa
     2821 gacatgattg tcctgtgtgt ctccccctca aaaatgctgg tgataagaga aatcaacagc
     2881 caattttgac tggagcaccc gttggacttg gaaatcctag ctctctaggg gtgggtcaac
     2941 agtctgcccc caacctaagc actgttagtc agattgatcc cagctccata gaaagagcct
     3001 atgcagctct tggactaccc tatcaagtaa atcagatgcc gacacaaccc caggtgcaag
     3061 caaagaacca gcagaatcag cagcctgggc agtctcccca aggcatgcgg cccatgagca
     3121 acatgagtgc tagtcctatg ggagtaaatg gaggtgtagg agttcaaacg ccgagtcttc
     3181 tttctgactc aatgttgcat tcagccataa attctcaaaa cccaatgatg agtgaaaatg
     3241 ccagtgtgcc ctccctgggt cctatgccaa cagcagctca accatccact actggaattc
     3301 ggaaacagtg gcacgaagat attactcagg atcttcgaaa tcatcttgtt cacaaactcg
     3361 tccaagccat atttcctacg ccggatcctg ctgctttaaa agacagacgg atggaaaacc
     3421 tagttgcata tgctcggaaa gttgaagggg acatgtatga atctgcaaac aatcgagcgg
     3481 aatactacca ccttctagct gagaaaatct ataagatcca gaaagaacta gaagaaaaac
     3541 gaaggaccag actacagaag cagaacatgc taccaaatgc tgcaggcatg gttccagttt
     3601 ccatgaatcc agggcctaac atgggacagc cgcaaccagg aatgacttct aatggccctc
     3661 tacctgaccc aagtatgatc cgtggcagtg tgccaaacca gatgatgcct cgaataactc
     3721 cacaatctgg tttgaatcaa tttggccaga tgagcatggc ccagccccct attgtacccc
     3781 ggcaaacccc tcctcttcag caccatggac agttggctca acctggagct ctcaacccgc
     3841 ctatgggcta tgggcctcgt atgcaacagc cttccaacca gggccagttc cttcctcaga
     3901 ctcagttccc atcacaggga atgaatgtaa caaatatccc tttggctccg tccagcggtc
     3961 aagctccagt gtctcaagca caaatgtcta gttcttcctg cccggtgaac tctcctataa
     4021 tgcctccagg gtctcagggg agccacattc actgtcccca gcttcctcaa ccagctcttc
     4081 atcagaattc accctcgcct gtacctagtc gtacccccac ccctcaccat actcccccaa
     4141 gcataggggc tcagcagcca ccagcaacaa caattccagc ccctgttcct acacctcctg
     4201 ccatgccacc tgggccacag tcccaggctc tacatccccc tccaaggcag acacctacac
     4261 caccaacaac acaacttccc caacaagtgc agccttcact tcctgctgca ccttctgctg
     4321 accagcccca gcagcagcct cgctcacagc agagcacagc agcgtctgtt cctaccccaa
     4381 cagcaccgct gcttcctccg cagcctgcaa ctccactttc ccagccagct gtaagcattg
     4441 aaggacaggt atcaaatcct ccatctacta gtagcacaga agtgaattct caggccattg
     4501 ctgagaagca gccttcccag gaagtgaaga tggaggccaa aatggaagtg gatcaaccag
     4561 aaccagcaga tactcagccg gaggatattt cagagtctaa agtggaagac tgtaaaatgg
     4621 aatctaccga aacagaagag agaagcactg agttaaaaac tgaaataaaa gaggaggaag
     4681 accagccaag tacttcagct acccagtcat ctccggctcc aggacagtca aagaaaaaga
     4741 ttttcaaacc agaagaacta cgacaggcac tgatgccaac tttggaggca ctttaccgtc
     4801 aggatccaga atcccttccc tttcgtcaac ctgtggaccc tcagctttta ggaatccctg
     4861 attactttga tattgtgaag agccccatgg atctttctac cattaagagg aagttagaca
     4921 ctggacagta tcaggagccc tggcagtatg tcgatgatat ttggcttatg ttcaataatg
     4981 cctggttata taaccggaaa acatcacggg tatacaaata ctgctccaag ctctctgagg
     5041 tctttgaaca agaaattgac ccagtgatgc aaagccttgg atactgttgt ggcagaaagt
     5101 tggagttctc tccacagaca ctgtgttgct acggcaaaca gttgtgcaca atacctcgtg
     5161 atgccactta ttacagttac cagaacaggt atcatttctg tgagaagtgt ttcaatgaga
     5221 tccaagggga gagcgtttct ttgggggatg acccttccca gcctcaaact acaataaata
     5281 aagaacaatt ttccaagaga aaaaatgaca cactggatcc tgaactgttt gttgaatgta
     5341 cagagtgcgg aagaaagatg catcagatct gtgtccttca ccatgagatc atctggcctg
     5401 ctggattcgt ctgtgatggc tgtttaaaga aaagtgcacg aactaggaaa gaaaataagt
     5461 tttctgctaa aaggttgcca tctaccagac ttggcacctt tctagagaat cgtgtgaatg
     5521 actttctgag gcgacagaat caccctgagt caggagaggt cactgttaga gtagttcatg
     5581 cttctgacaa aaccgtggaa gtaaaaccag gcatgaaagc aaggtttgtg gacagtggag
     5641 agatggcaga atcctttcca taccgaacca aagccctctt tgcctttgaa gaaattgatg
     5701 gtgttgacct gtgcttcttt ggcatgcatg ttcaagagta tggctctgac tgccctccac
     5761 ccaaccagag gagagtatac atatcttacc tcgatagtgt tcatttcttc cgtcctaaat
     5821 gcttgaggac tgcagtctat catgaaatcc taattggata tttagaatat gtcaagaaat
     5881 taggttacac aacagggcat atttgggcat gtccaccaag tgagggagat gattatatct
     5941 tccattgcca tcctcctgac cagaagatac ccaagcccaa gcgactgcag gaatggtaca
     6001 aaaaaatgct tgacaaggct gtatcagagc gtattgtcca tgactacaag gatattttta
     6061 aacaagctac tgaagataga ttaacaagtg caaaggaatt gccttatttc gagggtgatt
     6121 tctggcccaa tgttctggaa gaaagcatta aggaactgga acaggaggaa gaagagagaa
     6181 aacgagagga aaacaccagc aatgaaagca cagatgtgac caagggagac agcaaaaatg
     6241 ctaaaaagaa gaataataag aaaaccagca aaaataagag cagcctgagt aggggcaaca
     6301 agaagaaacc cgggatgccc aatgtatcta acgacctctc acagaaacta tatgccacca
     6361 tggagaagca taaagaggtc ttctttgtga tccgcctcat tgctggccct gctgccaact
     6421 ccctgcctcc cattgttgat cctgatcctc tcatcccctg cgatctgatg gatggtcggg
     6481 atgcgtttct cacgctggca agggacaagc acctggagtt ctcttcactc cgaagagccc
     6541 agtggtccac catgtgcatg ctggtggagc tgcacacgca gagccaggac cgctttgtct
     6601 acacctgcaa tgaatgcaag caccatgtgg agacacgctg gcactgtact gtctgtgagg
     6661 attatgactt gtgtatcacc tgctataaca ctaaaaacca tgaccacaaa atggagaaac
     6721 taggccttgg cttagatgat gagagcaaca accagcaggc tgcagccacc cagagcccag
     6781 gcgattctcg ccgcctgagt atccagcgct gcatccagtc tctggtccat gcttgccagt
     6841 gtcggaatgc caattgctca ctgccatcct gccagaagat gaagcgggtt gtgcagcata
     6901 ccaagggttg caaacggaaa accaatggcg ggtgccccat ctgcaagcag ctcattgccc
     6961 tctgctgcta ccatgccaag cactgccagg agaacaaatg cccggtgccg ttctgcctaa
     7021 acatcaagca gaagctccgg cagcaacagc tgcagcaccg actacagcag gcccaaatgc
     7081 ttcgcaggag gatggccagc atgcagcgga ctggtgtggt tgggcagcaa cagggcctcc
     7141 cttcccccac tcctgccact ccaacgacac caactggcca acagccaacc accccgcaga
     7201 cgccccagcc cacttctcag cctcagccta cccctcccaa tagcatgcca ccctacttgc
     7261 ccaggactca agctgctggc cctgtgtccc agggtaaggc agcaggccag gtgacccctc
     7321 caacccctcc tcagactgct cagccacccc ttccagggcc cccacctgca gcagtggaaa
     7381 tggcaatgca gattcagaga gcagcggaga cgcagcgcca gatggcccac gtgcaaattt
     7441 ttcaaaggcc aatccaacac cagatgcccc cgatgactcc catggccccc atgggtatga
     7501 acccacctcc catgaccaga ggtcccagtg ggcatttgga gccagggatg ggaccgacag
     7561 ggatgcagca acagccaccc tggagccaag gaggattgcc tcagccccag caactacagt
     7621 ctgggatgcc aaggccagcc atgatgtcag tggcccagca tggtcaacct ttgaacatgg
     7681 ctccacaacc aggattgggc caggtaggta tcagcccact caaaccaggc actgtgtctc
     7741 aacaagcctt acaaaacctt ttgcggactc tcaggtctcc cagctctccc ctgcagcagc
     7801 aacaggtgct tagtatcctt cacgccaacc cccagctgtt ggctgcattc atcaagcagc
     7861 gggctgccaa gtatgccaac tctaatccac aacccatccc tgggcagcct ggcatgcccc
     7921 aggggcagcc agggctacag ccacctacca tgccaggtca gcagggggtc cactccaatc
     7981 cagccatgca gaacatgaat ccaatgcagg cgggcgttca gagggctggc ctgccccagc
     8041 agcaaccaca gcagcaactc cagccaccca tgggagggat gagcccccag gctcagcaga
     8101 tgaacatgaa ccacaacacc atgccttcac aattccgaga catcttgaga cgacagcaaa
     8161 tgatgcaaca gcagcagcaa cagggagcag ggccaggaat aggccctgga atggccaacc
     8221 ataaccagtt ccagcaaccc caaggagttg gctacccacc acagcagcag cagcggatgc
     8281 agcatcacat gcaacagatg caacaaggaa atatgggaca gataggccag cttccccagg
     8341 ccttgggagc agaggcaggt gccagtctac aggcctatca gcagcgactc cttcagcaac
     8401 agatggggtc ccctgttcag cccaacccca tgagccccca gcagcatatg ctcccaaatc
     8461 aggcccagtc cccacaccta caaggccagc agatccctaa ttctctctcc aatcaagtgc
     8521 gctctcccca gcctgtccct tctccacggc cacagtccca gcccccccac tccagtcctt
     8581 ccccaaggat gcagcctcag ccttctccac accacgtttc cccacagaca agttccccac
     8641 atcctggact ggtagctgcc caggccaacc ccatggaaca agggcatttt gccagcccgg
     8701 accagaattc aatgctttct cagcttgcta gcaatccagg catggcaaac ctccatggtg
     8761 caagcgccac ggacctggga ctcagcaccg ataactcaga cttgaattca aacctctcac
     8821 agagtacact agacatacac gtttaaacga attcgggctc ggtacccggg gatcctctag
     8881 agtcgacctg caggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag
     8941 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc
     9001 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     9061 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     9121 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     9181 ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa
     9241 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     9301 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     9361 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     9421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9481 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9541 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9721 ggcttttgca aaaagcttaa ttaactgttg acaattaatc atcggcatag tatatcggca
     9781 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     9841 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     9901 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     9961 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
    10021 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
    10081 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
    10141 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
    10201 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
    10261 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
    10321 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
    10381 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
    10441 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
    10501 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
    10561 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
    10621 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc
    10681 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
    10741 gcgataagga tccgctcatg cgatgataag ctgtcaaaca tgagaattac aacttatatc
    10801 gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa atctagaaat
    10861 attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt
    10921 gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca
    10981 actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag
    11041 tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct
    11101 gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat
    11161 aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
    11221 gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat
    11281 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa
    11341 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc
    11401 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact
    11461 tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt
    11521 tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat
    11581 atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct gccacatgaa
    11641 gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac tccgctagcg
    11701 ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcatgagatt acgcgtttta
    11761 gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct cgtagccatc
    11821 gagataaacc gcagtactcc ggccacgatg cgtccggcgt agaggatcga gatct
//
