LOCUS       sequence-47851-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kerby RL, Youn H, Roberts GP
  TITLE     RcoM: a new single-component transcriptional regulator of CO 
            metabolism in bacteria.
  JOURNAL   J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7.
  PUBMED    18326575
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2008-05"; volume: "190(9)"; pages: "3336-43. 
            Epub 2008 Mar 7"
COMMENT     Sequence Label: UQ4802
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(51..56,57..72,73..79)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    87..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     92..114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta
       61 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
      121 aattcaggag gaatttaaaa tgaaatcgtc tgagcccgct tccgtatcgg cggccgagcg
      181 ccgcgcggag acgttccagc acaagctcga gcagttcaat ccggggatcg tctggctgga
      241 ccagcacggc cgcgtcacgg cattcaacga cgtcgcgctg caaattctcg ggccggccgg
      301 cgagcaatcg ctaggcgtgg cgcaggatag tctgttcggc atcgacgtgg tgcaactgca
      361 cccggagaaa agccgcgaca agctgcgctt cctgctgcag tcgaaagacg tgggtggctg
      421 cccggtcaag tcgccgccgc cggtggcgat gatgatcaat attcccgacc ggatcctgat
      481 gatcaaggtg tccagcatga tcgccgccgg cggcgcgtgc ggcacctgca tgatcttcta
      541 cgacgtcacc gatctgacca ccgagccctc cggcctgccg gcgggcggca gcgcgccctc
      601 gccgaggcgc ctcttcaaga ttcccgtgta ccggaagaac cgcgtgatcc tgctcgatct
      661 gaaagacatc gtgcgctttc agggcgacgg ccactacacg acgatcgtga cgcgggacga
      721 ccgctacctg tccaatcttt cactggccga tctggagctg cgtctcgaca gcagcattta
      781 tctgcgcgtt catcgcagcc atatcgtgag cctgcaatat gcggtggagc tggtcaaact
      841 ggacgagagc gtcaatctcg tca
//
