LOCUS       sequence-47853-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Kerby RL, Youn H, Roberts GP
  TITLE     RcoM: a new single-component transcriptional regulator of CO 
            metabolism in bacteria.
  JOURNAL   J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7.
  PUBMED    18326575
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2008-05"; volume: "190(9)"; pages: "3336-43. 
            Epub 2008 Mar 7"
COMMENT     Sequence Label: UQ4884
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(51..56,57..72,73..79)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    87..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     92..114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta
       61 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
      121 aattcaggag gaatttaaaa tgaaatcgtc agaatccgcc gccgcgacgg cttccgagcg
      181 ccgtgcagag acgttccagc acaagctcga acagttcaac ccggggatcg tctggctgga
      241 cccgcagggc cacgtaagcg cattcaacga cgtcgcgctg cacattctcg ggccggccgg
      301 cgagcaatcg ctcggcgtgg cgcaggatca tctgttcggg atcgacgtgg tgcaactgca
      361 tccggagaaa agccgcgaca agctgcgttt tctgctgcag tccagggacg cgggtggctg
      421 ccctgtcaga tcgccgccgc cggtggcgat gatgatcaac atcccggacc gcatcctgat
      481 gatcaaggtg tcgaaaatga ctggcgcggc gggcacctgc ggcagctgca tgatctttta
      541 cgacgtgacc gatctgacca ccgaaccgtc cagccagccg gcaggcgcca gcgtgccggc
      601 gccgcgccgt ctcttcaaga tccccgtgta ccggaagagc cgcgtgatcc tgatcgatct
      661 gaaggacatt gtgcgctttc agggcgacgg ccactacacg acgatcgtca cgaaagacga
      721 gcgctacctc tccaatcttt cgctggccga cctcgaactg cgcctcgata gcagcgtcta
      781 tctgcgggtt catcgcagcc atatcgtgag ccttccgtac gcggtcgaac tggtcaaact
      841 ggacgagagc gtcaatctcg tcatggacga cgcggaacaa cccaggtacc ggt
//
