LOCUS       sequence-478582-       12627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter and loxP flanked stop cassette, for cloning into AsiSI or 
            Mlui.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 12627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 CAG AsiSI/MluI
FEATURES             Location/Qualifiers
     source          1..12627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1080..5420
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     polyA_signal    5493..5627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5693..5917
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5906..5923
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     5937..5953
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5937..5953
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    6044..6077
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6079..6213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6443..7042)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6526..6546)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7023..7042
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    7139..7172
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          7219..8235
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7273..7295)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8236..8512)
                     /label=chicken beta-actin promoter
     enhancer        8514..8893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(8908..8955)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    9087..9221
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          9268..9333
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    join(9577..9614,9615..9680)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     misc_feature    9703..10786
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     promoter        complement(10825..10843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10853..10870)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10853..10869)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10862..10884)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(11010..11465)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11147..11168)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11359..11378
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        11492..11596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11597..11665,11666..12457)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11815..11834)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggatcaagc ttatcgatac cgtcgacggt atcgataagc
     1081 tagatgtcct gaaatatttc tatcagaaca aggtagtata aagctggtag gtatacaaaa
     1141 cgctagacta gtttctatcc ctgaccctta atctgctagt atatccgtag gaagttgctt
     1201 aagtgccact agtaccaaca gcctctgtta gagtgaacag tagattttaa tgaagggcca
     1261 ataaagacca cttaagacta ctgactgaat taaaaatcca cccagtggag ttagtattgg
     1321 ttatagttat ataacaaaat atccaactta gccaggcgtg gtggtgcacg cctttaatcc
     1381 cggcactcag gaggcagagg caggcggatt tctgagttcg aggccagcct ggtctacaaa
     1441 gtgagttcca ggacagccag ggctatacag agaaaccctg tctcgaaaaa ccaaaaaaaa
     1501 aaaaaaaaag gaaaaaaaat atggcttgaa aacatgctat tgttgtcaaa aggagatcca
     1561 aacaagtatt tctttgaaca aaaattatgt agaaaacagg gtgataaaga acaagggctt
     1621 tttaaaaaag gccagcatta tttgagatat acaagtgact ccaattcaag cctaaaggcc
     1681 actcaatgct cactaacagt gctaatcaga atcaattcct tagcccagtc aaaaatagtc
     1741 ctgaatatct tctggataaa catgtcctcc aacatataaa ctagtataca ctaacaaaac
     1801 gtctcaactt caaggtgaaa tgcttgactc ctagacttgt gacccagcaa agtgctctat
     1861 aggtagggtt actaggtcag agagtcttgc ctgcaaacca caattataca attccaccaa
     1921 atgtaagaca tgtaaaaatg acttatttag ctcaggtttt gaaaattaaa aatcaaaccc
     1981 acaaagacct aactttcaca ttaagtacaa atgtttaaca tatataacat gttttgcctt
     2041 gatatatgaa atcatatgac atcacctgac aggaaactta agtttatttg aacaacaatc
     2101 agcctaaggt aggcctagca catgatcagg atagtgcagg gaaacccaaa gaagtgcttc
     2161 tgagtataaa ccaagagacc tcgaaatagc agctttgttc tgtatctcat gggctttaac
     2221 tatcacttga aaacaatttc acaaatcaca aaaccaacat ttgtttagtt tccaactctg
     2281 cctattcacc ggagaaatcc atgtcccaat ttgatggggg aaaacttgaa tgaaatatta
     2341 tttggaatat tttaatatcc ctgtgtatat gcagatggtt taaagacagg taaccttaac
     2401 tgtagtttct tggttgtctg ccttgttttt tgaaatatgc tctcaccagg agcctgccaa
     2461 gtaactactc ttgtgtgctc agcaagtcct agggatccct taagcatgct ctaacaggcc
     2521 tggctttttt ttttaaaaag taggttgcgg tgataataat tcaagttttc atcacttgaa
     2581 acacatttta ccaactatca cccaagctcc cttagtgtgt cccctataaa agaatctgac
     2641 ctgcaagttc caaaagtatt actacatttt aaccttagta ttcaggagac aaaagcaggc
     2701 agaactttag tctacatatc ttgttgcaga tcaggcagag atacatagtg agaccctgcc
     2761 tcaggaaaag ctatcatttt tatattcaca gtaagcaaat taacattaaa agtcagaaat
     2821 tcttaatttg actttggctg tgaagaattt ggattctctg gatttgaaac tagaaaatct
     2881 gattaagcga tgcaacagtt tctgtatact aaaactctaa gacccttggt tctaaagata
     2941 ccacatttaa aaaaaaagtt aagtataaga caaaaagcct taatagctct tttatagggg
     3001 agggactcat ttaatattag tccacctcac tcctcataac tattttatga ggtttattat
     3061 ataacccatt ttttaaaaaa aaaattaaag gacaatttta gtgtttgaaa gatttcccaa
     3121 ccccaccctg gaaatcaggc tgcaaatctc agcagctgcc cttagaaggc aaaaagccat
     3181 gccatttaag ccatgggaag ttagtagcaa acaagagacc acatggcagg aaggaggctt
     3241 taaagaaagc ccacagtgtc aaaagaccca accaacagca gagacagaaa aaataatacc
     3301 caaatctcat cagaaaggta gacggattta gccacatcca tagtggctca ttagggaatg
     3361 cttcaattat aattttatga aatctcagag cccccctccc caacagggtt tctgtgtgta
     3421 ttcctggcta tcctagatag aacttgatgt gtagaccagg ctgggctcaa actcagagat
     3481 ctgcctgtct ctgcctccag agtgctggga ttaaaggcat aaaggcaagc accaccactg
     3541 gctggctaaa ctctggccct acatttttga aatccaaaat tatcattact tgctttcatg
     3601 tttagtaatg gctgcagact tagctttcag ctttgtatag atgaagcaca aaagttaagt
     3661 aaagctagca gtttaaacaa ataggatact agaagttcaa tttttatttc tttattgctt
     3721 agtgtaagag aaaagacatt aaagtctgca ttggtcttct gtatcccaca agtctgcagt
     3781 tatggctcct ctgtccacag ttacacttca aataaaaatt agttccttgg taacatttcc
     3841 agtgcttaaa gagtaatttg attattggct accatattgg aacaaacaca aagtatttca
     3901 ttacttcagt aaattctact ggagacagta ttctctttaa aaaaaaaaaa aagaagaaga
     3961 aggaggattt atttatttaa tgtgaataca cttgtggtct tcagacacac cagaagaggg
     4021 catcagatcc cattacagat ggttgtgagc caccatgtgg ttgctgggaa ttgaactcag
     4081 gacttctgga agagcagtct gtgctcttga actccgagcc acctctccag cccctggata
     4141 gtattattat agagcacaag cacacacaac acaacaacct aaaaaccaag cttcacaaat
     4201 acatttgttg cttaatcatt ttacttgtgt ttggctacaa gtcaagcaaa attataggtc
     4261 ctgaagaagc ttggcaaaat cacatttaga ccagcaataa cgtgtagaat gccatgagtc
     4321 aagccagtcc aagagaaagc actgcttaaa tagaaaaaat acaagctcac aagaccttag
     4381 gtcaggaaag acaacaaaca cctgaacttt gcattccaaa aggaaccacc ttttacagat
     4441 gtgtacaatg acaaaatttt agtaagcagt aatcaatacc atgtggctca ataatgaaat
     4501 atacctttta atgtcttaaa atatcagcct ggcaatatgt aagatacatc aggtaatatt
     4561 gggggaggag acatccacct ggaaaccatt aatggttaat agctcagttt ataaatggag
     4621 aaaaaggaga gaggcattca tgggagtgga aagttaagct ttaggttgga ttctcaatac
     4681 atctattgtt gtaaaactcc ctggactgag aataggccca aatgtggaac accacctgac
     4741 gggagaggtg atagacactg aaaattaagg atcaaggcaa aggatcaaca aaaagtgtac
     4801 taaggagtta taaaagaact gcagtgttga ggccccagct acagcctcga tttgtggtgt
     4861 atgtaactaa tctgtctggt ttcatgagtc atcagacttc taagatcagg aaagggaaaa
     4921 tgccaatgct ctgtctaggg gttggataag ccagtataat aaatgaaaat ggggctaaaa
     4981 tgagtgttct aaaatacctt ttgataaggc tgcagaagga gcgggagaaa tggatatgaa
     5041 gtactgggct ctttaaaaat gattaaaatt ctgcttacat agtctaactc gcgacactgt
     5101 aatttcatac tgtagtaagg atctcaagca ggagagtata aaactcgggt gagcatgtct
     5161 ttaatctacc tcgatggaaa atactccgag gcggatcaca agcaataata acctgtagtt
     5221 ttgctgcata aaaccccaga tgactaccta tcctcccatt ttccttattt gcccctatta
     5281 aaaaacttcc cgacaaaacc gaaaatctgt gggaagtctt gtccctccaa ttttacacct
     5341 gttcaattcc cctgcaggac aacgcccaca caccaggtta gcctttaagc ctgcccagaa
     5401 gactcccgcc catcttctag ggccgcgaat taaaaaacct cccacacctc cccctgaacc
     5461 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     5521 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5581 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctac gtaatacgac
     5641 tcactatagt tctagaggct cgagccccag ctggttcttt ccgcctcaga agccatagag
     5701 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
     5761 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     5821 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     5881 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcta
     5941 gaactagtgg atctgcaatc atttacgcgt gcgatcgcaa ttccagagga actttggtca
     6001 aggcctagct ggcccaagct actcgcgact atggtggccc ggtataactt cgtataatgt
     6061 atgctatacg aagttatgga tccagacatg ataagataca ttgatgagtt tggacaaacc
     6121 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6181 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     6241 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     6301 ggtatggctg attatgatcc tctagagtcg gtgggcctcg ggggcgggtg cggggtcggc
     6361 ggggccgccc cgggtggctt cggtcggagc catggggtcg tgcgctcctt tcggtcgggc
     6421 gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg
     6481 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
     6541 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
     6601 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
     6661 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
     6721 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
     6781 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
     6841 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
     6901 cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg
     6961 cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca
     7021 ccgtgggctt gtactcggtc atggaaggtc gtctccttgt gaggggtcag gggcgtgggt
     7081 caggggatgg tggcggcacc ggccggtcgt ggcggccgac ctgcaggcat gcaagcttat
     7141 aacttcgtat aatgtatgct atacgaagtt attcgaactg taccggttgt tacaggctta
     7201 agacggttaa ttaaggatct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc
     7261 tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag
     7321 gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg
     7381 cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc
     7441 gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccgcac agatttggga
     7501 caaaggaagt ccctgcgccc tctcgcacga ttaccataaa aggcaatggc tgcggctcgc
     7561 cgcgcctcga cagccgccgg cgctccgggg gccgccgcgc ccctcccccg agccctcccc
     7621 ggcccgaggc ggccccgccc cgcccggcac ccccacctgc cgccaccccc cgcccggcac
     7681 ggcgagcccc gcgccacgcc ccgtacggag ccccgcaccc gaagccgggc cgtgctcagc
     7741 aactcgggga ggggggtgca gggggggttg cagcccgacc gacgcgccca caccccctgc
     7801 tcaccccccc acgcacacac cccgcacgca gcctttgttc ccctcgcagc cccccccgca
     7861 ccgcggggca ccgcccccgg ccgcgctccc ctcgcgcaca ctgcggagcg cacaaagccc
     7921 cgcgccgcgc ccgcagcgct cacagccgcc gggcagcgcg gagccgcacg cggcgctccc
     7981 cacgcacaca cacacgcacg caccccccga gccgctcccc ccgcacaaag ggccctcccg
     8041 gagcccctca aggctttcac gcagccacag aaaagaaaca agccgtcatt aaaccaagcg
     8101 ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga gtaacgcggt
     8161 cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca
     8221 gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc gccgccgccg
     8281 cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg ccgcacctct
     8341 ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccg cctggcgcgc gccccccccc
     8401 ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa aattgggggt
     8461 ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc gaccatggta
     8521 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
     8581 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     8641 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     8701 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     8761 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     8821 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagtc
     8881 aataatcaat gtcgaatccg gcgcgccgaa gttcctatac tttctagaga ataggaactt
     8941 cggaatagga acttcaagct taagcgctag cacggccgca gatcataatc agccatacca
     9001 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     9061 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     9121 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9181 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctggcattcc accactgctc
     9241 ccattcatca gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt
     9301 aacacattat acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag
     9361 tttgtccaat tatgtcacac cacagaagta aggttccttc acaaagatcc atgcagatcc
     9421 cccctgcccg gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg
     9481 ctcagctgga attgtgattt aaatttaatt aagtggcccg gcaggtgggc ccgtacggcc
     9541 actagagcgg ccagcttatc gataccgtcg atccccactg gaaagaccgc gaagagtttg
     9601 tcctcaaccg cgagctgtgg aaaaaaaagg gacaggataa gtatgacatc atcaaggaaa
     9661 ccctggacta ctgcgcccta cagatctgca gcccgggcgc gcctagaaag actggagttg
     9721 cagatcacga gggaagaggg ggaagggatt ctcccaggcc cagggcggtc ctcagaagcc
     9781 aggaggcagc agagaactcc cagaaaggta ttgcaacact cccctccccc ctccggagaa
     9841 gggtgcggcc ttctccccgc ctactccact gcagctccct tactgataac aactcagagc
     9901 gactttggga gagcaagtgc ttcctgcctc caaaacagcc caactgagcc ctcgctcctt
     9961 ccctccactc cccggagtgc gcgatggagg tctggctcag cacgcccctc ttgaggcaac
    10021 tcaagtcgga aacgtgcttg cacccgcccc gcagccgctc agccctactg cccgtccccg
    10081 cccccagcgc gcgcttcctg ccacgttgcg caggggcgcg cggccagact ctgcggcgcg
    10141 gggccgaggg gagggccgga acctgggagc gcctcctcgc cgcccccgct ggccggcgga
    10201 tggactcaac ttgcacgaac acgagccaat ggcaagggcc agttttctgg gccccgagag
    10261 ccaatcagac gacgaggccc ggccggcggc gggtaaaacg actcccccag aggaagggga
    10321 gggtgggcgg ccgctcgcgc gcgagctact ttcgctgacc ctccctcccc tcccccgccc
    10381 cgccagaggc cgaccgcgcc cgcacgtcca gctcgcctca ccccacctac ctcccgcccc
    10441 acccagtggg cagagcgagg ctgccggcgg ctgcgcactc cggctgccgt taactgacag
    10501 gcgccttacg ccaaccaaaa cacgccattt gtgttttcac acacggcggg aggaaaagaa
    10561 gccaatcagc gacgagacgt cggccggaag cgctcctccg ctgccccccc cccccccgag
    10621 ccatggccgc gtccggtgga gacttttccg ctcccttctc cctccccctc cggttgctgc
    10681 agggcggacc gcattcctgc ccaccacccg cttgcccctt ccagcgtcac gactcgtacc
    10741 cggctgtctc acagaacggc tccaccacgc tcggagggcc tgccgctagg gataacaggg
    10801 taatgcggtg gagctccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
    10861 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    10921 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    10981 aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt
    11041 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
    11101 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
    11161 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
    11221 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
    11281 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
    11341 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
    11401 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
    11461 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    11521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    11581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    11641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    11701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    11761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    11821 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    11881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    11941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    12001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    12061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    12121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    12181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    12241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    12301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    12361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    12421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    12481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    12541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    12601 ccccgtagaa aagatcaaag gatcttc
//
