LOCUS       sequence-478605-       11168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Ubc promoter expressing iRFP670 mRNA taged 24xMS2 motif and
            24xPP7 motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Zhou WJ, Wu MY, Shao XJ, Tang LJ, Wang F, Jiang JH
  TITLE     Fluorogenic Interacting Protein Stabilization for Orthogonal RNA 
            Imaging.
  JOURNAL   Angew Chem Int Ed Engl. 2025 Apr 10:e202502350. doi: 
            10.1002/anie.202502350.
  PUBMED    40208703
REFERENCE   2  (bases 1 to 11168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2025 Apr 10:e202502350. doi: 10.1002/anie.202502350."
COMMENT     Sequence Label: Ubc-iRFP670-24xMS2-24xPP7
FEATURES             Location/Qualifiers
     source          1..11168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2624..2633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2630..3565
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     polyA_signal    7068..7202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7146..7165
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(7310..7343)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7396..7412)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7428..7450)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7558..8146
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7611..7631)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8029..8040)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8221..8454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8573..8591)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8659..8763
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8764..8832,8833..9624)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8982..9001)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9795..10383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10284..10303
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10537..10554
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10794..10815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10830..10835,10836..10853,10854..10860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10868..10884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10873..10895
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10892..10908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10892..10908
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgcgccacca tggcgcgtaa
     2641 ggtcgatctc acctcctgcg atcgcgagcc gatccacatc cccggcagca ttcagccgtg
     2701 cggctgcctg ctagcctgcg acgcgcaggc ggtgcggatc acgcgcatta cggaaaatgc
     2761 cggcgcgttc tttggacgcg aaactccgcg ggtcggtgag ctactcgccg attacttcgg
     2821 cgagaccgaa gcccatgcgc tgcgcaacgc actggcgcag tcctccgatc caaagcgacc
     2881 ggcgctgatc ttcggttggc gcgacggcct gaccggccgc accttcgaca tctcactgca
     2941 tcgccatgac ggtacatcga tcatcgagtt cgagcctgcg gcggccgaac aggccgacaa
     3001 tccgctgcgg ctgacgcggc agatcatcgc gcgcaccaaa gaactgaagt cgctcgaaga
     3061 gatggccgca cgggtgccgc gctatctgca ggcgatgctc ggctatcacc gcgtgatgtt
     3121 gtaccgcttc gcggacgacg gctccgggat ggtgatcggc gaggcgaagc gcagcgacct
     3181 cgagagcttt ctcggtcagc actttccggc gtcgctggtc ccgcagcagg cgcggctact
     3241 gtacttgaag aacgcgatcc gcgtggtctc ggattcgcgc ggcatcagca gccggatcgt
     3301 gcccgagcac gacgcctccg gcgccgcgct cgatctgtcg ttcgcgcacc tgcgcagcat
     3361 ctcgccctgc catctcgaat ttctgcggaa catgggcgtc agcgcctcga tgtcgctgtc
     3421 gatcatcatt gacggcacgc tatggggatt gatcatctgt catcattacg agccgcgtgc
     3481 cgtgccgatg gcgcagcgcg tcgcggccga aatgttcgcc gacttcttat cgctgcactt
     3541 caccgccgcc caccaccaac gctgagctag ctaggcggac tgttactgag ctgcgtttta
     3601 caccctttct ttgacaaaac ctaacttgcg cagaaaaaaa aaaaataaga gacaacattg
     3661 gcatggcttt gtttttttaa atttttttta aagttttttt tttttttttt tttttttaag
     3721 tttttttgtt ttgttttggc gcttttgact caggatttaa aaactggaac ggtgaaggcg
     3781 acagcagttg gttggagcaa acatccccca aagttctaca aatgtggctg aggactttgt
     3841 acattgtttt gttttttttt tttttggttt tgtctttttt taatagtcat tccaagtatg
     3901 tcgacagatc ctacctgcaa agcaccagag gatatggcct cggtgcctca gcatcttcag
     3961 atgtgctgtc actgtccaat cttcaagcca gcagagcata tgggctcgct ggctccactg
     4021 tcattacagt ggacctgatc ttctccaatc aaaggagcag aggatatggc ctcgctccct
     4081 ccgactacat caggctggaa gtgacatcca tcattcatcc cagcagagca tatgggctcg
     4141 ctgggaccac agcatgcctc gaagtgctga gagactatca tcaaaccgac agaggatatg
     4201 gcctcgtcgg ctcctactcc ctcctggtgg aatacttctc cactcttcaa cccagcagag
     4261 catatgggct cgctgggtcc atcagagata caagatctct cgacagatcc tacctgcaaa
     4321 gcaccagagg atatggcctc ggtgcctcag catcttcaga tgtgctgtca ctgtccaatc
     4381 ttcaagccag cagagcatat gggctcgctg gctccactgt cattacagtg gacctgatct
     4441 tctccaatca aaggagcaga ggatatggcc tcgctccctc cgactacatc aggctggaag
     4501 tgacatccat cattcatccc agcagagcat atgggctcgc tgggaccaca gcatgcctcg
     4561 aagtgctgag agactatcat caaaccgaca gaggatatgg cctcgtcggc tcctactccc
     4621 tcctggtgga atacttctcc actcttcaac ccagcagagc atatgggctc gctgggtcca
     4681 tcagagatac aagatctctc gacagatcct acctgcaaag caccagagga tatggcctcg
     4741 gtgcctcagc atcttcagat gtgctgtcac tgtccaatct tcaagccagc agagcatatg
     4801 ggctcgctgg ctccactgtc attacagtgg acctgatctt ctccaatcaa aggagcagag
     4861 gatatggcct cgctccctcc gactacatca ggctggaagt gacatccatc attcatccca
     4921 gcagagcata tgggctcgct gggaccacag catgcctcga agtgctgaga gactatcatc
     4981 aaaccgacag aggatatggc ctcgtcggct cctactccct cctggtggaa tacttctcca
     5041 ctcttcaacc cagcagagca tatgggctcg ctgggtccat cagagataca agatctctcg
     5101 acagatccta cctgcaaagc accagaggat atggcctcgg tgcctcagca tcttcagatg
     5161 tgctgtcact gtccaatctt caagccagca gagcatatgg gctcgctggc tccactgtca
     5221 ttacagtgga cctgatcttc tccaatcaaa ggagcagagg atatggcctc gctccctccg
     5281 actacatcag gctggaagtg acatccatca ttcatcccag cagagcatat gggctcgctg
     5341 ggaccacagc atgcctcgaa gtgctgagag actatcatca aaccgacaga ggatatggcc
     5401 tcgtcggctc ctactccctc ctggtggaat acttctccac tcttcaaccc agcagagcat
     5461 atgggctcgc tgggtccatc agagatacaa gatctctcga gccgcggtgc gtaagtctgt
     5521 taacagaatg ctgcatacgc tgagtacttc gaaatgtcat cctctgtctg acactgaacg
     5581 taatccagac gcggatccta cctacaaacg gcacgagcat cagccgtgcc gcccagcatc
     5641 ttcatcactg tccaatcttc aagcgtgcac gatcacggca cgctccactg tcattacagt
     5701 cttatccaat caaactccag cagcatcagc gctggagctc cgactacatc aggacatcca
     5761 tcattcaacg ctcgacgatc acgcgagcgt ccacagcatg cctcagacac tatcatcaaa
     5821 cggctgcacg atcacggcag ccgctcctac tgcctctacg ttatcgactc ttctgcgacg
     5881 agcatcagcc gtcgcaacat cggacatacc agatcctacc tacaaacggc acgagcatca
     5941 gccgtgccgc ccagcatctt catcactgtc caatcttcaa gcgtgcacga tcacggcacg
     6001 ctccactgtc attacagtct tatccaatca aactccagca gcatcagcgc tggagctccg
     6061 actacatcag gacatccatc attcaacgct cgacgatcac gcgagcgtcc acagcatgcc
     6121 tcagacacta tcatcaaacg gctgcacgat cacggcagcc gctcctactg cctctacgtt
     6181 atcgactctt ctgcgacgag catcagccgt cgcaacatcg gacataccag atcctaccta
     6241 caaacggcac gagcatcagc cgtgccgccc agcatcttca tcactgtcca atcttcaagc
     6301 gtgcacgatc acggcacgct ccactgtcat tacagtctta tccaatcaaa ctccagcagc
     6361 atcagcgctg gagctccgac tacatcagga catccatcat tcaacgctcg acgatcacgc
     6421 gagcgtccac agcatgcctc agacactatc atcaaacggc tgcacgatca cggcagccgc
     6481 tcctactgcc tctacgttat cgactcttct gcgacgagca tcagccgtcg caacatcgga
     6541 cataccagat cctacctaca aacggcacga gcatcagccg tgccgcccag catcttcatc
     6601 actgtccaat cttcaagcgt gcacgatcac ggcacgctcc actgtcatta cagtcttatc
     6661 caatcaaact ccagcagcat cagcgctgga gctccgacta catcaggaca tccatcattc
     6721 aacgctcgac gatcacgcga gcgtccacag catgcctcag acactatcat caaacggctg
     6781 cacgatcacg gcagccgctc ctactgcctc tacgttatcg actcttctgc gacgagcatc
     6841 agccgtcgca acatcggaca taccagatct tcaatcgatt tcgcgcggga tccagaccac
     6901 ctcccctgcg agctaagctg gacagccaat gacgggtaag agagtgacat ttttcactaa
     6961 cctaagacag gagggccgtc agagctactg cctaatccaa agacgggtaa aagtgataaa
     7021 aatgtatcac tccaacctaa gacaggcgca gcttccgagg gatttgagat ccagacatga
     7081 taagatacat tgatgagttt ggacaaacca aaactagaat gcagtgaaaa aaatgcctta
     7141 tttgtgaaat ttgtgatgct attgccttat ttgtaaccat tataagctgc aataaacaag
     7201 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     7261 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctg aagttcctat
     7321 actatttgaa gaataggaac ttcggaatag gaacttcgga tctcatatgg ccatatgggg
     7381 gcgcctagag aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga
     7441 ggttcaaggg ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtcaaac
     7501 ggtgcaggat caccgcaccg tacatcaaac acctcagatc tcatatcgat agatcctaat
     7561 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7621 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7681 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7741 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7801 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7861 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7921 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7981 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8041 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8101 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     8161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8521 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     8581 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     8641 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8701 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8761 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8821 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8881 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8941 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9001 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9061 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9121 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9181 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9241 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9301 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9361 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9421 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9481 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9541 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9601 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9661 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9721 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9781 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9841 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9901 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9961 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10021 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10081 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10141 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10201 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10261 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10321 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10381 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10441 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10501 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10561 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    10621 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    10681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    10741 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10801 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10861 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
    10921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10981 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    11041 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    11101 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    11161 ttagtagt
//
