LOCUS       sequence-47862-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  TITLE     Gary Roberts unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: UQ5457
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(51..56,57..72,73..79)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    87..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     92..114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta
       61 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
      121 aattcaggag gaatttaaaa tgcaccatca ccatcaccat aaatcgtctg agcccgcttc
      181 cgtatcggcg gccgagcgcc gcgcggagac gttccagcac aagctcgagc agttcaatcc
      241 ggggatcgtc tggctggacc agcacggccg cgtcacggca ttcaacgacg tcgcgctgca
      301 aattctcggg ccggccggcg agcaatcgct aggcgtggcg caggatagtc tgttcggcat
      361 cgacgtggtg caactgcacc cggagaaaag ccgcgacaag ctgcgcttcc tgctgcagtc
      421 gaaagacgtg ggtggctgcc cggtcaagtc gccgccgccg gtggcgatga tgatcaatat
      481 tcccgaccgg atcctgatga tcaaggtgtc cagcatgatc gccgccggcg gcgcgtgcgg
      541 cacctgcatg atcttctacg acgtcaccga tctgaccacc gagccctccg gcctgccggc
      601 gggcggcagc gcgccctcgc cgaggcgcct cttcaagatt cccgtgtacc ggaagaaccg
      661 cgtgatcctg ctcgatctga aagacatcgt gcgctttcag ggcgacggcc actacacgac
      721 gatcgtgacg cgggacgacc gctacctgtc caatctttca ctggccgatc tggagctgcg
      781 tctcgacagc agcatttatc tgcgcgttca tcgcagccat atcgtgagcc tgcaatatgc
      841 ggtggagctg gtcaaactgg acgagagcgt caatctcgtc atggacgacg cggaacagac
      901 ccaggtgccg gtcagcc
//
