LOCUS       sequence-47864-e       10960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Glover CP, Heywood DJ, Bienemann AS, Deuschle U, Kew JN, Uney JB
  TITLE     Adenoviral expression of CREB protects neurons from apoptotic and 
            excitotoxic stress.
  JOURNAL   Neuroreport. 2004 May 19;15(7):1171-5.
  PUBMED    15129168
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroreport."; date: "2004-05-19"; volume: "15(7)"; pages: "1171-5"
COMMENT     Sequence Label: pXCX.CMV.CREB.WPRE
FEATURES             Location/Qualifiers
     source          1..10960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..107
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     195..345
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    470..694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     683..700
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    727..1315
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             833..844
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1242..1262
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1364..2077)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1404..1424)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     2019..2047
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     regulatory      2074..2083
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2122..2673
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2124..2696
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2283..2302)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2512..2529
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        complement(3751..3769)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3812..3831)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          3814..3946
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(4113..4131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4177..4380)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4206..4226)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4381..4760
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             8516..8707
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(8701..8723)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8809..8949
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8864..8883
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8964..8981)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9135..9723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9215..9234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9894..10685,10686..10754))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10517..10536
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10755..10859)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10927..10945
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattcatcat caataatata ccttattttg gattgaagcc aatatgataa tgagggggtg
       61 gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag tgtggcggaa
      121 gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa aagtgacgtt
      181 tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta ggcggatgtt
      241 gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa actgaataag
      301 aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaatatttg tctagggccg
      361 cggggacttt gaccgtttac gtggagactc gcccaggtgt ttttctcagg tgttttccgc
      421 gttccgggtc aaagttggcg ttttattatt atagtcagtc tagctggttc catagagccc
      481 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
      541 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
      601 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
      661 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcgtac cgagctcgaa
      721 ttccaggcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
      781 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
      841 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
      901 gccatggaaa ggacgtcagc ttccccgaca acaccacgga attgtcagtg cccaacagcc
      961 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     1021 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     1081 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     1141 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     1201 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     1261 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattgtcga
     1321 cggatcctct agatgcatgc tcgagcggcc gccctcgagt ttatttgtat agttcatcca
     1381 tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt
     1441 cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct ggtaaaagga
     1501 cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga acgcttccat
     1561 cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc attcttttgt ttgtctgcca
     1621 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
     1681 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
     1741 tgacttcagc acgtgtcttg tagttcccgt catctttgaa aaatatagtt ctttcctgta
     1801 cataaccttc gggcatggca ctcttgaaaa agtcatgccg tttcatatga tctgggtatc
     1861 tcgcaaagca ttgaagacca tacgcaaaag tagtgacaag tgttggccat ggaacaggta
     1921 gttttccagt agtgcaaata aatttaaggg taagttttcc gtatgttgca tcaccttcac
     1981 cctctccact gacagaaaat ttgtgcccat taacatcacc atctaattca acaagaattg
     2041 ggacaactcc agtgaaaagt tcttctcctt tacccatggt ggcggcccgg tcgactctag
     2101 ctagaggtac cacgcgtgcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg
     2161 tggttcgggg ggcctagacg ttttttaacc tcgactaaac acatgtaaag catgtgcacc
     2221 gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt
     2281 tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc
     2341 actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc
     2401 acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc
     2461 ttcaagaagc ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt
     2521 tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg
     2581 ccctcacatt gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc
     2641 ggccttattc caagcggctt cggccagtaa cgttaggggg gggggaggga gaggggcgga
     2701 attcttaatc tgacttgtgg cagtaaaggt ccttaagtgc ttttagctcc tcaatcaatg
     2761 ttttgttttg gttttcaagc actgccactc tgttctctaa acatttcaca tattctttct
     2821 tctttctacg acattctctt gctgcttccc tgttcttcat tagacgaacc tctctctttc
     2881 gtgctgcttc ttcagcaggc tgtgtaggaa gtgctgggga ggacgccata acaactccag
     2941 gggcaatggt gctagtgggt gctgtgcgaa tctggtatgt ttgtacatca ccagaggcag
     3001 cttgaacaac aacttggttg ctgggcacta gaatctgctg tccatcagtg gtctgtgcat
     3061 attgtagaat ggtagtaccc ggctgagtgg cagctgcatt ggtcatggtt aatgtctgca
     3121 ggccctgtac cccatcggta ccattgttag ccagctgtat tgctcctccc tgggtaatgg
     3181 caatatactg cccactgcta gtttggtaaa tcggggttgg cactgttaca gtggtgatgg
     3241 caggggctga agtctcttct tctgattttt cttcttcaat ccttggcacc cctggtgcat
     3301 cagaagataa gtcattcaaa attttcctgt aggaaggcct ccttgaaagg atttcccttc
     3361 gtttttggga atcagttaca ctatccacag actcctgtga atcttcactt tctgcaatag
     3421 ttgaaatctg agttccggag aaaagtcttt ttaagtcctt acaggaagac tgaactgttt
     3481 ggacttgtgg agactgaata actgatggct gggccgcctg gatgacccca tggacctgga
     3541 ctgtctgccc attgggcagc tgcactaagg ttacagtggg agcagatgac gtcgcatgag
     3601 ctgctggcat ggatacctgg gctaatgtgg caatctgtgg ctgggcttga actgtcattt
     3661 gttgactttc agcttctgtt acagcagcat ctccactctg ctggttgtct gctccagagt
     3721 ccatggtcat ggtggaattc tcgaggctag cctatagtga gtcgtattaa gtactctagc
     3781 cttaagagct gtaattgaac tgggagtgga cacctgtgga gagaaaggca aagtggatgt
     3841 cagtaagacc aataggtgcc tatcagaaac gcaagagtct tctctgtctc gacaagccca
     3901 gtttctattg gtctccttaa acctgtcttg taaccttgat acttacctgc ccagtgcctc
     3961 acgaccaact tctgcagctt aagttcgaga ctgttgtgtc agaagcactg actgcgttag
     4021 caatttaact gtgataaact accgcaataa agcttctagt gatctgacgg ttcactaaac
     4081 ggatccgagc tcggtaccaa gcttccggtc tccctatagt gagtcgtatt aatttcgata
     4141 agccagttaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
     4201 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     4261 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     4321 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     4381 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     4441 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     4501 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     4561 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     4621 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     4681 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     4741 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgctag angatctgga
     4801 aggtgctgag gtacgatgag acccgcacca ggtgcagacc ctgcgagtgt ggcggtaaac
     4861 atattaggaa ccagcctgtg atgctggatg tgaccgagga gctgaggccc gatcacttgg
     4921 tgctggcctg cacccgcgct gagtttggct ctagcgatga agatacagat tgaggtactg
     4981 aaatgtgtgg gcgtggctta agggtgggaa agaatatata aggtgggggt cttatgtagt
     5041 tttgtatctg ttttgcagca gccgccgccg ccatgagcac caactcgttt gatggaagca
     5101 ttgtgagctc atatttgaca acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga
     5161 tgggctccag cattgatggt cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg
     5221 agaccgtgtc tggaacgccg ttggagactg cagcctccgc cgccgcttca gccgctgcag
     5281 ccaccgcccg cgggattgtg actgactttg ctttcctgag cccgcttgca agcagtgcag
     5341 cttcccgttc atccgcccgc gatgacaagt tgacggctct tttggcacaa ttggattctt
     5401 tgacccggga acttaatgtc gtttctcagc agctgttgga tctgcgccag caggtttctg
     5461 ccctgaaggc ttcctcccct cccaatgcgg tttaaaacat aaataaaaaa ccagactctg
     5521 tttggatttg gatcaagcaa gtgtcttgct gtctttattt aggggttttg cgcgcgcggt
     5581 aggcccggga ccagcggtct cggtcgttga gggtcctgtg tattttttcc aggacgtggt
     5641 aaaggtgact ctggatgttc agatacatgg gcataagccc gtctctgggg tggaggtagc
     5701 accactgcag agcttcatgc tgcggggtgg tgttgtagat gatccagtcg tagcaggagc
     5761 gctgggcgtg gtgcctaaaa atgtctttca gtagcaagct gattgccagg ggcaggccct
     5821 tggtgtaagt gtttacaaag cggttaagct gggatgggtg catacgtggg gatatgagat
     5881 gcatcttgga ctgtattttt aggttggcta tgttcccagc catatccctc cggggattca
     5941 tgttgtgcag aaccaccagc acagtgtatc cggtgcactt gggaaatttg tcatgtagct
     6001 tagaaggaaa tgcgtggaag aacttggaga cgcccttgtg acctccaaga ttttccatgc
     6061 attcgtccat aatgatggca atgggcccac gggcggcggc ctgggcgaag atatttctgg
     6121 gatcactaac gtcatagttg tgttccagga tgagatcgtc ataggccatt tttacaaagc
     6181 gcgggcggag ggtgccagac tgcggtataa tggttccatc cggcccaggg gcgtagttac
     6241 cctcacagat ttgcatttcc cacgctttga gttcagatgg ggggatcatg tctacctgcg
     6301 gggcgatgaa gaaaacggtt tccggggtag gggagatcag ctgggaagaa agcaggttcc
     6361 tgagcagctg cgacttaccg cagccggtgg gcccgtaaat cacacctatt accgggtgca
     6421 actggtagtt aagagagctg cagctgccgt catccctgag caggggggcc acttcgttaa
     6481 gcatgtccct gactcgcatg ttttccctga ccaaatccgc cagaaggcgc tcgccgccca
     6541 gcgatagcag ttcttgcaag gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag
     6601 gcatgctttt gagcgtttga ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct
     6661 ctacggcatc tcgatccagc atatctcctc gtttcgcggg ttggggcggc tttcgctgta
     6721 cggcagtagt cggtgctcgt ccagacgggc cagggtcatg tctttccacg ggcgcagggt
     6781 cctcgtcagc gtagtctggg tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag
     6841 ggtgcgcttg aggctggtcc tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc
     6901 ggccaggtag catttgacca tggtgtcata gtccagcccc tccgcggcgt ggcccttggc
     6961 gcgcagcttg cccttggagg aggcgccgca cgaggggcag tgcagacttt tgagggcgta
     7021 gagcttgggc gcgagaaata ccgattccgg ggagtaggca tccgcgccgc aggccccgca
     7081 gacggtctcg cattccacga gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt
     7141 tcccccatgc tttttgatgc gtttcttacc tctggtttcc atgagccggt gtccacgctc
     7201 ggtgacgaaa aggctgtccg tgtccccgta tacagacttg agaggcctgt cctcgaccga
     7261 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     7321 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     7381 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     7441 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     7501 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     7561 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     7621 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     7681 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac
     7741 cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     7801 ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     7861 gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     7921 aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     7981 catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg
     8041 gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
     8101 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
     8161 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
     8221 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
     8281 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
     8341 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
     8401 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
     8461 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
     8521 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
     8581 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
     8641 cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa
     8701 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     8761 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     8821 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     8881 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     8941 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9001 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9061 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9121 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9181 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9241 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9301 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9361 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9421 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9481 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9541 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9601 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9721 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9781 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9841 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9901 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9961 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10021 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10081 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10141 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10201 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10261 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10321 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10381 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10441 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10501 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10561 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10621 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10681 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10741 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10861 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    10921 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
//
