LOCUS       sequence-478650-       10285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TOM20-HK2 lentiviral overexpression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10285)
  TITLE     Cantor Lab lentiviral overexpression vector
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLJC2-TOM20-HK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3199..3402
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3353..3373
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     RBS             6007..6015
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6323..6388
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6436..6553
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6602..7112
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7031..7049
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7134..7733
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7134..7153)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7630..7650
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7861..8094
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8166..8287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8203..8222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8257..8276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8327..8462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8482..8501)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8483..8501)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8511..8528)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8511..8527)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8520..8542)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8669..9124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8756..8775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8966..8987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9150..9254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9255..9323,9324..10115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcagcagcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3181 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3241 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3301 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3361 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3421 atccgctagc gctaccggtt taattaacgg caggatggtg ggtcggaaca gcgccatcgc
     3481 cgccggtgta tgcggggccc ttttcattgg gtactgcatc tacttcgacc gcaaaagacg
     3541 aagtggaggc agcattgcct cgcatctgct tgcctacttc ttcacggagc tcaaccatga
     3601 ccaagtgcag aaggttgacc agtatctcta ccacatgcgc ctctctgatg agaccctctt
     3661 ggagatctct aagcggttcc gcaaggagat ggagaaaggg cttggagcca ccactcaccc
     3721 tactgcagca gtgaagatgc tgcccacctt tgtgaggtcc actccagatg ggacagaaca
     3781 cggagagttc ctggctctgg atcttggagg gaccaacttc cgtgtgcttt gggtgaaagt
     3841 aacggacaat gggctccaga aggtggagat ggagaatcag atctatgcca tccctgagga
     3901 catcatgcga ggcagtggca cccagctgtt tgaccatatc gccgagtgtc tggcaaactt
     3961 catggataag ctacaaatca aagacaagaa gctcccactg ggttttacct tctcgttccc
     4021 ctgccaccag actaaactag acgagagttt cctggtctca tggaccaagg gattcaagtc
     4081 cagtggagtg gaaggcagag acgttgtggc tctgatccgg aaggccatcc agaggagagg
     4141 ggactttgat atcgacattg tggctgtggt gaatgacaca gttgggacca tgatgacctg
     4201 tggttatgat gaccacaact gtgagattgg tctcattgtg ggcacgggca gcaacgcctg
     4261 ctacatggaa gagatgcgcc acatcgacat ggtggaaggc gatgaggggc ggatgtgcat
     4321 caatatggag tggggggcct tcggggacga tggctcgctc aacgacattc gcactgagtt
     4381 tgaccaggag attgacatgg gctcactgaa cccgggaaag caactgtttg agaagatgat
     4441 cagtgggatg tacatggggg agctggtgag gcttatcctg gtgaagatgg ccaaggagga
     4501 gctgctcttt ggggggaagc tcagcccaga gcttctcaac accggtcgct ttgagaccaa
     4561 agacatctca gacattgaag gggagaagga tggcatccgg aaggcccgtg aggtcctgat
     4621 gcggttgggc ctggacccga ctcaggagga ctgcgtggcc actcaccgga tctgccagat
     4681 cgtgtccaca cgctccgcca gcctgtgcgc agccaccctg gccgccgtgc tgcagcgcat
     4741 caaggagaac aaaggcgagg agcggctgcg ctctactatt ggggtcgacg gttccgtcta
     4801 caagaaacac ccccattttg ccaagcgtct acataagacc gtgcggcggc tggtgcccgg
     4861 ctgcgatgtc cgcttcctcc gctccgagga tggcagtggc aaaggtgcag ccatggtgac
     4921 agcagtggct taccggctgg ccgatcaaca ccgtgcccgc cagaagacat tagagcatct
     4981 gcagctgagc catgaccagc tgctggaggt caagaggagg atgaaggtag aaatggagcg
     5041 aggtctgagc aaggagactc atgccagtgc ccccgtcaag atgctgccca cctacgtgtg
     5101 tgctaccccg gacggcacag agaaagggga cttcttggcc ttggaccttg gaggaacaaa
     5161 tttccgggtc ctgctggtcc gtgttcggaa tgggaagtgg ggtggagtgg agatgcacaa
     5221 caagatctac gccatcccgc aggaggtcat gcacggcacc ggggacgagc tctttgacca
     5281 cattgtccag tgcatcgcgg acttcctcga gtacatgggc atgaagggcg tgtccctgcc
     5341 tctgggtttt accttctcct tcccctgcca gcagaacagc ctggacgaga gcatcctcct
     5401 caagtggaca aaaggcttca aggcatctgg ctgcgagggc gaggacgtgg tgaccctgct
     5461 gaaggaagcg atccaccggc gagaggagtt tgacctggat gtggttgctg tggtgaacga
     5521 cacagtcgga actatgatga cctgtggctt tgaagaccct cactgtgaag ttggcctcat
     5581 tgttggcacg ggcagcaatg cctgctacat ggaggagatg cgcaatgtgg aactggtgga
     5641 aggagaagag gggcggatgt gtgtgaacat ggaatggggg gccttcgggg acaatggatg
     5701 cctagatgac ttccgcacag aatttgatgt ggctgtggat gagctttcac tcaacccggg
     5761 caagcagagg ttcgagaaaa tgatcagtgg aatgtacctg ggtgagattg tccgtaacat
     5821 tctcatcgat ttcaccaagc gtggactact cttccgaggc cgcatctcag agcggctcaa
     5881 gacaaggggc atctttgaaa ccaagttctt gtctcagatt gagagtgact gcctggccct
     5941 gctgcaagtc cgagccatcc tgcaacactt agggcttgag agcacctgtg acgacagcat
     6001 cattgttaag gaggtgtgca ctgtggtggc ccggcgggca gcccagctct gtggcgcagg
     6061 catggccgct gtggtggaca ggatacgaga aaaccgtggg ctggacgctc tcaaagtgac
     6121 agtgggtgtg gatgggaccc tctacaagct acatcctcac tttgccaaag tcatgcatga
     6181 gacagtgaag gacctggctc cgaaatgtga tgtgtctttc ctgcagtcag aggatggcag
     6241 cgggaagggg gcggcgctca tcactgctgt ggcctgccgc atccgtgagg ctggacagcg
     6301 aggaggaagt gcggccgcag gcgactacaa agaccatgac ggtgattata aagatcatga
     6361 cattgattac aaggatgacg atgacaagta ataagaattc tcgacctcga gacaaatggc
     6421 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     6481 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     6541 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     6601 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     6661 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     6721 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     6781 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     6841 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     6901 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     6961 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     7021 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     7081 tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggagctt accatgaccg
     7141 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     7201 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     7261 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     7321 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     7381 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     7441 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7501 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7561 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7621 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7681 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     7741 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa
     7801 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7861 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8101 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8161 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8281 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8341 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8401 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8461 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     8521 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8581 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     8641 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     8701 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8761 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8821 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8881 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8941 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9001 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9061 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9121 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9181 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9241 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9481 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9781 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10261 ccgtagaaaa gatcaaagga tcttc
//
