LOCUS       sequence-478666-       10862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HK1 lentiviral overexpression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10862)
  TITLE     Cantor Lab lentiviral overexpression vector
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLJC5-HK1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2881..4092
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3929..3948
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6900..6965
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7013..7130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7179..7689
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7608..7626
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7711..8310
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7711..7730)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8207..8227
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8438..8671
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8743..8864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8780..8799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8834..8853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8904..9039
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9059..9078)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9060..9078)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9088..9105)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9088..9104)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9097..9119)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9246..9701
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9333..9352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9543..9564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9727..9831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9832..9900,9901..10692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcgggatct
     2881 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
     2941 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
     3001 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
     3061 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
     3121 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
     3181 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
     3241 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
     3301 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
     3361 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
     3421 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
     3481 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     3541 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3601 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     3661 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     3721 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3781 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3841 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     3901 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     3961 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     4021 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     4081 tttttgttag acgaagcgct agcgctaccg gtttaattaa cgccagcatg atcgccgcgc
     4141 agctcctggc ctattacttc acggagctga aggatgacca ggtcaaaaag attgacaagt
     4201 atctgtatgc catgcggctc tccgatgaaa ctctcataga tatcatgact cgcttcagga
     4261 aggagatgaa gaatggcctc tcccgggatt ttaatccaac agccacagtc aagatgttgc
     4321 caacattcgt aaggtccatt cctgatggct ctgaaaaggg agatttcatt gccctggatc
     4381 ttggtgggtc ttcctttcga attctgcggg tgcaagtgaa tcatgagaaa aaccagaatg
     4441 ttcacatgga gtccgaggtt tatgacaccc cagagaacat cgtgcacggc agtggaagcc
     4501 agctttttga tcatgttgct gagtgcctgg gagatttcat ggagaaaagg aagatcaagg
     4561 acaagaagtt acctgtggga ttcacgtttt cttttccttg ccaacaatcc aaaatagatg
     4621 aggccatcct gatcacctgg acaaagcgat ttaaagcgag cggagtggaa ggagcagatg
     4681 tggtcaaact gcttaacaaa gccatcaaaa agcgagggga ctatgatgcc aacatcgtag
     4741 ctgtggtgaa tgacacagtg ggcaccatga tgacctgtgg ctatgacgac cagcactgtg
     4801 aagtcggcct gatcatcggc actggcacca atgcttgcta catggaggaa ctgaggcaca
     4861 ttgatctggt ggaaggagac gaggggagga tgtgtatcaa tacagaatgg ggagcctttg
     4921 gagacgatgg atcattagaa gacatccgga cagagtttga cagggagata gaccggggat
     4981 ccctcaaccc tggaaaacag ctgtttgaga agatggtcag tggcatgtac ttgggagagc
     5041 tggttcgact gatcctagtc aagatggcca aggagggcct cttatttgaa gggcggatca
     5101 ccccggagct gctcacccga gggaagttta acaccagtga tgtgtcagcc atcgaaaaga
     5161 ataaggaagg cctccacaat gccaaagaaa tcctgacccg cctgggagtg gagccgtccg
     5221 atgatgactg tgtctcagtc cagcacgttt gcaccattgt ctcatttcgc tcagccaact
     5281 tggtggctgc cacactgggc gccatcttga accgcctgcg tgataacaag ggcacaccca
     5341 ggctgcggac cacggttggt gtcgacggat ctctttacaa gacgcaccca cagtattccc
     5401 ggcgtttcca caagactcta aggcgcttgg tgccagactc cgatgtgcgc ttcctcctct
     5461 cggagagtgg cagcggcaag ggggctgcca tggtgacggc ggtggcctac cgcttggccg
     5521 agcagcaccg gcagatagag gagaccctgg ctcatttcca cctcaccaaa gacatgctgc
     5581 tggaggtgaa gaagaggatg cgggccgaga tggagctggg gctgaggaag cagacgcaca
     5641 acaatgccgt ggttaagatg ctgccctcct tcgtccggag aactcccgac gggaccgaga
     5701 atggtgactt cttggccctg gatcttggag gaaccaattt ccgtgtgctg ctggtgaaaa
     5761 tccgtagtgg gaaaaagaga acggtggaaa tgcacaacaa gatctacgcc attcctattg
     5821 aaatcatgca gggcactggg gaagagctgt ttgatcacat tgtctcctgc atctctgact
     5881 tcttggacta catggggatc aaaggcccca ggatgcctct gggcttcacg ttctcatttc
     5941 cctgccagca gacgagtctg gacgcgggaa tcttgatcac gtggacaaag ggttttaagg
     6001 caacagactg cgtgggccac gatgtagtca ccttactaag ggatgcgata aaaaggagag
     6061 aggaatttga cctggacgtg gtggctgtgg tcaacgacac agtgggcacc atgatgacct
     6121 gtgcttatga ggagcccacc tgtgaggttg gactcattgt tgggaccggc agcaatgcct
     6181 gctacatgga ggagatgaag aacgtggaga tggtggaggg ggaccagggg cagatgtgca
     6241 tcaacatgga gtggggggcc tttggggaca acgggtgtct ggatgatatc aggacacact
     6301 acgacagact ggtggacgaa tattccctaa atgctgggaa acaaaggtat gagaagatga
     6361 tcagtggtat gtacctgggt gaaatcgtcc gcaacatctt aatcgacttc accaagaagg
     6421 gattcctctt ccgagggcag atctctgaga cgctgaagac ccggggcatc tttgagacca
     6481 agtttctctc tcagatcgag agtgaccgat tagcactgct ccaggtccgg gctatcctcc
     6541 agcagctagg tctgaatagc acctgcgatg acagtatcct cgtcaagaca gtgtgcgggg
     6601 tggtgtccag gagggccgca cagctgtgtg gcgcaggcat ggctgcggtt gtggataaga
     6661 tccgcgagaa cagaggactg gaccgtctga atgtgactgt gggagtggac gggacactct
     6721 acaagcttca tccacacttc tccagaatca tgcaccagac ggtgaaggaa ctgtcaccaa
     6781 aatgtaacgt gtccttcctc ctgtctgagg atggcagcgg caagggggcc gccctcatca
     6841 cggccgtggg cgtgcggtta cgcacagagg caagcagcgg aggcagcgcg gccgcaggcg
     6901 actacaaaga ccatgacggt gattataaag atcatgacat tgattacaag gatgacgatg
     6961 acaagtaata agaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
     7021 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     7081 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     7141 acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc
     7201 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     7261 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     7321 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     7381 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     7441 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     7501 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     7561 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     7621 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     7681 tctccccagg gggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc
     7741 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7801 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7861 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7921 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7981 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8041 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8101 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8161 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8221 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8281 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     8341 gaaaggagcg cacgacccca tgcatcggta cctttaagac caatgactta caaggcagct
     8401 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8461 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8521 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8581 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8641 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8701 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8761 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8881 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8941 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     9001 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     9061 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     9121 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     9181 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9241 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9301 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9361 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9421 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9481 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9541 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9601 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9661 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     9721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9841 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9901 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9961 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    10021 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    10081 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10141 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    10201 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    10261 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10321 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    10381 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    10441 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10501 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10561 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10621 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10681 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10741 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10801 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10861 tc
//
