LOCUS       sequence-478679-       10685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TOM20-HK2 lentiviral overexpression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  TITLE     Cantor Lab lentiviral overexpression vector
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLJC6-TOM20-HK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2881..4092
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3929..3948
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     RBS             6680..6688
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6996..7061
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7109..7226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7275..7785
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7704..7722
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7794..8189
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8261..8494
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8566..8687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8603..8622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8657..8676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8727..8862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8882..8901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8883..8901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8911..8928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8911..8927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8920..8942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9069..9524
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9156..9175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9366..9387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9550..9654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9655..9723,9724..10515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcgggatct
     2881 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
     2941 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
     3001 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
     3061 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
     3121 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
     3181 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
     3241 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
     3301 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
     3361 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
     3421 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
     3481 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     3541 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3601 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     3661 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     3721 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3781 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3841 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     3901 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     3961 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     4021 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     4081 tttttgttag acgaagcgct agcgctaccg gtttaattaa cggcaggatg gtgggtcgga
     4141 acagcgccat cgccgccggt gtatgcgggg cccttttcat tgggtactgc atctacttcg
     4201 accgcaaaag acgaagtgga ggcagcattg cctcgcatct gcttgcctac ttcttcacgg
     4261 agctcaacca tgaccaagtg cagaaggttg accagtatct ctaccacatg cgcctctctg
     4321 atgagaccct cttggagatc tctaagcggt tccgcaagga gatggagaaa gggcttggag
     4381 ccaccactca ccctactgca gcagtgaaga tgctgcccac ctttgtgagg tccactccag
     4441 atgggacaga acacggagag ttcctggctc tggatcttgg agggaccaac ttccgtgtgc
     4501 tttgggtgaa agtaacggac aatgggctcc agaaggtgga gatggagaat cagatctatg
     4561 ccatccctga ggacatcatg cgaggcagtg gcacccagct gtttgaccat atcgccgagt
     4621 gtctggcaaa cttcatggat aagctacaaa tcaaagacaa gaagctccca ctgggtttta
     4681 ccttctcgtt cccctgccac cagactaaac tagacgagag tttcctggtc tcatggacca
     4741 agggattcaa gtccagtgga gtggaaggca gagacgttgt ggctctgatc cggaaggcca
     4801 tccagaggag aggggacttt gatatcgaca ttgtggctgt ggtgaatgac acagttggga
     4861 ccatgatgac ctgtggttat gatgaccaca actgtgagat tggtctcatt gtgggcacgg
     4921 gcagcaacgc ctgctacatg gaagagatgc gccacatcga catggtggaa ggcgatgagg
     4981 ggcggatgtg catcaatatg gagtgggggg ccttcgggga cgatggctcg ctcaacgaca
     5041 ttcgcactga gtttgaccag gagattgaca tgggctcact gaacccggga aagcaactgt
     5101 ttgagaagat gatcagtggg atgtacatgg gggagctggt gaggcttatc ctggtgaaga
     5161 tggccaagga ggagctgctc tttgggggga agctcagccc agagcttctc aacaccggtc
     5221 gctttgagac caaagacatc tcagacattg aaggggagaa ggatggcatc cggaaggccc
     5281 gtgaggtcct gatgcggttg ggcctggacc cgactcagga ggactgcgtg gccactcacc
     5341 ggatctgcca gatcgtgtcc acacgctccg ccagcctgtg cgcagccacc ctggccgccg
     5401 tgctgcagcg catcaaggag aacaaaggcg aggagcggct gcgctctact attggggtcg
     5461 acggttccgt ctacaagaaa cacccccatt ttgccaagcg tctacataag accgtgcggc
     5521 ggctggtgcc cggctgcgat gtccgcttcc tccgctccga ggatggcagt ggcaaaggtg
     5581 cagccatggt gacagcagtg gcttaccggc tggccgatca acaccgtgcc cgccagaaga
     5641 cattagagca tctgcagctg agccatgacc agctgctgga ggtcaagagg aggatgaagg
     5701 tagaaatgga gcgaggtctg agcaaggaga ctcatgccag tgcccccgtc aagatgctgc
     5761 ccacctacgt gtgtgctacc ccggacggca cagagaaagg ggacttcttg gccttggacc
     5821 ttggaggaac aaatttccgg gtcctgctgg tccgtgttcg gaatgggaag tggggtggag
     5881 tggagatgca caacaagatc tacgccatcc cgcaggaggt catgcacggc accggggacg
     5941 agctctttga ccacattgtc cagtgcatcg cggacttcct cgagtacatg ggcatgaagg
     6001 gcgtgtccct gcctctgggt tttaccttct ccttcccctg ccagcagaac agcctggacg
     6061 agagcatcct cctcaagtgg acaaaaggct tcaaggcatc tggctgcgag ggcgaggacg
     6121 tggtgaccct gctgaaggaa gcgatccacc ggcgagagga gtttgacctg gatgtggttg
     6181 ctgtggtgaa cgacacagtc ggaactatga tgacctgtgg ctttgaagac cctcactgtg
     6241 aagttggcct cattgttggc acgggcagca atgcctgcta catggaggag atgcgcaatg
     6301 tggaactggt ggaaggagaa gaggggcgga tgtgtgtgaa catggaatgg ggggccttcg
     6361 gggacaatgg atgcctagat gacttccgca cagaatttga tgtggctgtg gatgagcttt
     6421 cactcaaccc gggcaagcag aggttcgaga aaatgatcag tggaatgtac ctgggtgaga
     6481 ttgtccgtaa cattctcatc gatttcacca agcgtggact actcttccga ggccgcatct
     6541 cagagcggct caagacaagg ggcatctttg aaaccaagtt cttgtctcag attgagagtg
     6601 actgcctggc cctgctgcaa gtccgagcca tcctgcaaca cttagggctt gagagcacct
     6661 gtgacgacag catcattgtt aaggaggtgt gcactgtggt ggcccggcgg gcagcccagc
     6721 tctgtggcgc aggcatggcc gctgtggtgg acaggatacg agaaaaccgt gggctggacg
     6781 ctctcaaagt gacagtgggt gtggatggga ccctctacaa gctacatcct cactttgcca
     6841 aagtcatgca tgagacagtg aaggacctgg ctccgaaatg tgatgtgtct ttcctgcagt
     6901 cagaggatgg cagcgggaag ggggcggcgc tcatcactgc tgtggcctgc cgcatccgtg
     6961 aggctggaca gcgaggagga agtgcggccg caggcgacta caaagaccat gacggtgatt
     7021 ataaagatca tgacattgat tacaaggatg acgatgacaa gtaataagaa ttctcgacct
     7081 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     7141 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     7201 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     7261 ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     7321 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     7381 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     7441 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     7501 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     7561 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     7621 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     7681 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     7741 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tccatggttc
     7801 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     7861 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     7921 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     7981 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     8041 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     8101 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     8161 tgccctctgg ttatgtgtgg gagggctaag gtacctttaa gaccaatgac ttacaaggca
     8221 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8281 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8341 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8401 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8461 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8521 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8701 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8761 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8821 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     8881 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     8941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9061 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9481 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     9541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10681 tcttc
//
