LOCUS       sequence-478681-       10189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HK2 lentiviral overexpression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10189)
  TITLE     Cantor Lab lentiviral overexpression vector
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLJC2-HK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3199..3402
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3353..3373
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     RBS             5911..5919
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6227..6292
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6340..6457
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6506..7016
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6935..6953
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7038..7637
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7038..7057)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7534..7554
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7765..7998
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8070..8191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8107..8126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8161..8180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8231..8366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8386..8405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8387..8405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8415..8432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8415..8431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8424..8446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8573..9028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8660..8679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8870..8891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9054..9158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9159..9227,9228..10019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcagcagcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3181 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3241 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3301 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3361 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3421 atccgctagc gctaccggtt taattaacgg caggatgatt gcctcgcatc tgcttgccta
     3481 cttcttcacg gagctcaacc atgaccaagt gcagaaggtt gaccagtatc tctaccacat
     3541 gcgcctctct gatgagaccc tcttggagat ctctaagcgg ttccgcaagg agatggagaa
     3601 agggcttgga gccaccactc accctactgc agcagtgaag atgctgccca cctttgtgag
     3661 gtccactcca gatgggacag aacacggaga gttcctggct ctggatcttg gagggaccaa
     3721 cttccgtgtg ctttgggtga aagtaacgga caatgggctc cagaaggtgg agatggagaa
     3781 tcagatctat gccatccctg aggacatcat gcgaggcagt ggcacccagc tgtttgacca
     3841 tatcgccgag tgtctggcaa acttcatgga taagctacaa atcaaagaca agaagctccc
     3901 actgggtttt accttctcgt tcccctgcca ccagactaaa ctagacgaga gtttcctggt
     3961 ctcatggacc aagggattca agtccagtgg agtggaaggc agagacgttg tggctctgat
     4021 ccggaaggcc atccagagga gaggggactt tgatatcgac attgtggctg tggtgaatga
     4081 cacagttggg accatgatga cctgtggtta tgatgaccac aactgtgaga ttggtctcat
     4141 tgtgggcacg ggcagcaacg cctgctacat ggaagagatg cgccacatcg acatggtgga
     4201 aggcgatgag gggcggatgt gcatcaatat ggagtggggg gccttcgggg acgatggctc
     4261 gctcaacgac attcgcactg agtttgacca ggagattgac atgggctcac tgaacccggg
     4321 aaagcaactg tttgagaaga tgatcagtgg gatgtacatg ggggagctgg tgaggcttat
     4381 cctggtgaag atggccaagg aggagctgct ctttgggggg aagctcagcc cagagcttct
     4441 caacaccggt cgctttgaga ccaaagacat ctcagacatt gaaggggaga aggatggcat
     4501 ccggaaggcc cgtgaggtcc tgatgcggtt gggcctggac ccgactcagg aggactgcgt
     4561 ggccactcac cggatctgcc agatcgtgtc cacacgctcc gccagcctgt gcgcagccac
     4621 cctggccgcc gtgctgcagc gcatcaagga gaacaaaggc gaggagcggc tgcgctctac
     4681 tattggggtc gacggttccg tctacaagaa acacccccat tttgccaagc gtctacataa
     4741 gaccgtgcgg cggctggtgc ccggctgcga tgtccgcttc ctccgctccg aggatggcag
     4801 tggcaaaggt gcagccatgg tgacagcagt ggcttaccgg ctggccgatc aacaccgtgc
     4861 ccgccagaag acattagagc atctgcagct gagccatgac cagctgctgg aggtcaagag
     4921 gaggatgaag gtagaaatgg agcgaggtct gagcaaggag actcatgcca gtgcccccgt
     4981 caagatgctg cccacctacg tgtgtgctac cccggacggc acagagaaag gggacttctt
     5041 ggccttggac cttggaggaa caaatttccg ggtcctgctg gtccgtgttc ggaatgggaa
     5101 gtggggtgga gtggagatgc acaacaagat ctacgccatc ccgcaggagg tcatgcacgg
     5161 caccggggac gagctctttg accacattgt ccagtgcatc gcggacttcc tcgagtacat
     5221 gggcatgaag ggcgtgtccc tgcctctggg ttttaccttc tccttcccct gccagcagaa
     5281 cagcctggac gagagcatcc tcctcaagtg gacaaaaggc ttcaaggcat ctggctgcga
     5341 gggcgaggac gtggtgaccc tgctgaagga agcgatccac cggcgagagg agtttgacct
     5401 ggatgtggtt gctgtggtga acgacacagt cggaactatg atgacctgtg gctttgaaga
     5461 ccctcactgt gaagttggcc tcattgttgg cacgggcagc aatgcctgct acatggagga
     5521 gatgcgcaat gtggaactgg tggaaggaga agaggggcgg atgtgtgtga acatggaatg
     5581 gggggccttc ggggacaatg gatgcctaga tgacttccgc acagaatttg atgtggctgt
     5641 ggatgagctt tcactcaacc cgggcaagca gaggttcgag aaaatgatca gtggaatgta
     5701 cctgggtgag attgtccgta acattctcat cgatttcacc aagcgtggac tactcttccg
     5761 aggccgcatc tcagagcggc tcaagacaag gggcatcttt gaaaccaagt tcttgtctca
     5821 gattgagagt gactgcctgg ccctgctgca agtccgagcc atcctgcaac acttagggct
     5881 tgagagcacc tgtgacgaca gcatcattgt taaggaggtg tgcactgtgg tggcccggcg
     5941 ggcagcccag ctctgtggcg caggcatggc cgctgtggtg gacaggatac gagaaaaccg
     6001 tgggctggac gctctcaaag tgacagtggg tgtggatggg accctctaca agctacatcc
     6061 tcactttgcc aaagtcatgc atgagacagt gaaggacctg gctccgaaat gtgatgtgtc
     6121 tttcctgcag tcagaggatg gcagcgggaa gggggcggcg ctcatcactg ctgtggcctg
     6181 ccgcatccgt gaggctggac agcgaggagg aagtgcggcc gcaggcgact acaaagacca
     6241 tgacggtgat tataaagatc atgacattga ttacaaggat gacgatgaca agtaataaga
     6301 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     6361 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     6421 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     6481 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     6541 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     6601 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     6661 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     6721 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     6781 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     6841 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     6901 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     6961 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     7021 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     7081 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     7141 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     7201 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     7261 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     7321 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7381 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7441 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7501 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7561 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7621 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     7681 gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7741 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7801 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7861 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7921 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7981 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8041 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     8101 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8161 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8221 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8281 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8341 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     8401 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8461 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8521 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     8581 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8641 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8701 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8761 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8821 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8881 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8941 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9001 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     9061 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9181 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9241 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9301 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9361 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9421 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9481 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9541 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9601 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9661 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9721 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9781 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9841 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9901 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9961 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    10021 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10141 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
