LOCUS       sequence-478691-       13089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of dFnCas12a in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Yang LZ, Min YH, Liu YX, Gao BQ, Liu XQ, Huang Y, Wang H, Yang L, 
            Liu ZJ, Chen LL
  TITLE     CRISPR-array-mediated imaging of non-repetitive and multiplex 
            genomic loci in living cells.
  JOURNAL   Nat Methods. 2024 Sep;21(9):1646-1657. doi: 
            10.1038/s41592-024-02333-3. Epub 2024 Jul 4.
  PUBMED    38965442
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-024-02333-3"; journalName: "Nat Methods"; date: 
            "2024-09"; volume: "21"; issue: "9"; pages: "1646-1657"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag
FEATURES             Location/Qualifiers
     source          1..13089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      7386..7395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7392..7394,7395..7397,7398..8108)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7392..7394,7395..7397,7398..8108)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7437..7458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7698..7717)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8045..8066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8136..8183
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8202..8267
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8274..8841
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8435..8452)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8662..8681
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8861..9460
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8861..8880)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9357..9377
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9479..10067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9532..9552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9950..9961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10142..10375
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10494..10512)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10580..10684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10685..10753,10754..11545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10903..10922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11716..12304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12205..12224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12458..12475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12715..12736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12751..12756,12757..12774,12775..12781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12789..12805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12794..12816
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12813..12829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12813..12829
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12879..12898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcagc atctaccagg
     3481 agttcgtcaa caagtattca ctgagtaaga cactgcggtt cgagctgatc ccacagggca
     3541 agacactgga gaacatcaag gcccgaggcc tgattctgga cgatgagaag cgggcaaaag
     3601 actataagaa agccaagcag atcattgata aataccacca gttctttatc gaggaaattc
     3661 tgagctccgt gtgcatcagt gaggatctgc tgcagaatta ctcagacgtg tacttcaagc
     3721 tgaagaagag cgacgatgac aacctgcaga aggacttcaa gtccgccaag gacaccatca
     3781 agaaacagat tagcgagtac atcaaggact ccgaaaagtt taaaaatctg ttcaaccaga
     3841 atctgatcga tgctaagaaa ggccaggagt ccgacctgat cctgtggctg aaacagtcta
     3901 aggacaatgg gattgaactg ttcaaggcta actccgatat cactgatatt gacgaggcac
     3961 tggaaatcat caagagcttc aagggatgga ccacatactt taaaggcttc cacgagaacc
     4021 gcaagaacgt gtactccagc aacgacattc ctacctccat catctaccga atcgtcgatg
     4081 acaatctgcc aaagttcctg gagaacaagg ccaaatatga atctctgaag gacaaagctc
     4141 ccgaggcaat taattacgaa cagatcaaga aagatctggc tgaggaactg acattcgata
     4201 tcgactataa gactagcgag gtgaaccaga gggtcttttc cctggacgag gtgtttgaaa
     4261 tcgccaattt caacaattac ctgaaccagt ccggcattac taaattcaat accatcattg
     4321 gcgggaagtt tgtgaacggg gagaatacca agcgcaaggg aattaacgaa tacatcaatc
     4381 tgtatagcca gcagatcaac gacaaaactc tgaagaaata caagatgtct gtgctgttca
     4441 aacagatcct gagtgatacc gagtccaagt cttttgtcat tgataaactg gaagatgact
     4501 cagacgtggt cactaccatg cagagctttt atgagcagat cgccgctttc aagacagtgg
     4561 aggaaaaatc tattaaggaa actctgagtc tgctgttcga tgacctgaaa gcccagaagc
     4621 tggacctgag taagatctac ttcaaaaacg ataagagtct gacagacctg tcacagcagg
     4681 tgtttgatga ctattccgtg attgggaccg ccgtcctgga gtacattaca cagcagatcg
     4741 ctccaaagaa cctggataat ccctctaaga aagagcagga actgatcgct aagaaaaccg
     4801 agaaggcaaa atatctgagt ctggaaacaa ttaagctggc actggaggag ttcaacaagc
     4861 acagggatat tgacaaacag tgccgctttg aggaaatcct ggccaacttc gcagccatcc
     4921 ccatgatttt tgatgagatc gcccagaaca aagacaatct ggctcagatc agtattaagt
     4981 accagaacca gggcaagaaa gacctgctgc aggcttcagc agaagatgac gtgaaagcca
     5041 tcaaggatct gctggaccag accaacaatc tgctgcacaa gctgaaaatc ttccatatta
     5101 gtcagtcaga ggataaggct aatatcctgg ataaagacga acacttctac ctggtgttcg
     5161 aggaatgtta cttcgagctg gcaaacattg tccccctgta taacaagatt aggaactaca
     5221 tcacacagaa gccttactct gacgagaagt ttaaactgaa cttcgaaaat agtaccctgg
     5281 ccaacgggtg ggataagaac aaggagcctg acaacacagc tatcctgttc atcaaggatg
     5341 acaagtacta tctgggagtg atgaataaga aaaacaataa gatcttcgat gacaaagcca
     5401 ttaaggagaa caaaggggaa ggatacaaga aaatcgtgta taagctgctg cccggcgcaa
     5461 ataagatgct gcctaaggtg ttcttcagcg ccaagagtat caaattctac aacccatccg
     5521 aggacatcct gcggattaga aatcactcaa cacatactaa gaacgggagc ccccagaagg
     5581 gatatgagaa atttgagttc aacatcgagg attgcaggaa gtttattgac ttctacaagc
     5641 agagcatctc caaacaccct gaatggaagg attttggctt ccggttttcc gacacacaga
     5701 gatataactc tatcgacgag ttctaccgcg aggtggaaaa tcaggggtat aagctgactt
     5761 ttgagaacat ttctgaaagt tacatcgaca gcgtggtcaa tcagggaaag ctgtacctgt
     5821 tccagatcta taacaaagat ttttcagcat acagcaaggg cagaccaaac ctgcatacac
     5881 tgtactggaa ggccctgttc gatgagagga atctgcagga cgtggtctat aaactgaacg
     5941 gagaggccga actgttttac cggaagcagt ctattcctaa gaaaatcact cacccagcta
     6001 aggaggccat cgctaacaag aacaaggaca atcctaagaa agagagcgtg ttcgaatacg
     6061 atctgattaa ggacaagcgg ttcaccgaag ataagttctt tttccattgt ccaatcacca
     6121 ttaacttcaa gtcaagcggc gctaacaagt tcaacgacga gatcaatctg ctgctgaagg
     6181 aaaaagcaaa cgatgtgcac atcctgagca ttgcccgagg agagcggcat ctggcctact
     6241 ataccctggt ggatggcaaa gggaatatca ttaagcagga tacattcaac atcattggca
     6301 atgaccggat gaaaaccaac taccacgata aactggctgc aatcgagaag gatagagact
     6361 cagctaggaa ggactggaag aaaatcaaca acattaagga gatgaaggaa ggctatctga
     6421 gccaggtggt ccatgagatt gcaaagctgg tcatcgaata caatgccatt gtggtgttcg
     6481 cggatctgaa cttcggcttt aagagggggc gctttaaggt ggaaaaacag gtctatcaga
     6541 agctggagaa aatgctgatc gaaaagctga attacctggt gtttaaagat aacgagttcg
     6601 acaagaccgg aggcgtcctg agagcctacc agctgacagc tccctttgaa actttcaaga
     6661 aaatgggaaa acagacaggc atcatctact atgtgccagc cggattcact tccaagatct
     6721 gccccgtgac cggctttgtc aaccagctgt accctaaata tgagtcagtg agcaagtccc
     6781 aggaattttt cagcaagttc gataagatct gttataatct ggacaagggg tacttcgagt
     6841 tttccttcga ttacaagaac ttcggcgaca aggccgctaa ggggaaatgg accattgcct
     6901 ccttcggatc tcgcctgatc aactttcgaa attccgataa aaaccacaat tgggacacta
     6961 gggaggtgta cccaaccaag gagctggaaa agctgctgaa agactactct atcgagtatg
     7021 gacatggcga atgcatcaag gcagccatct gtggcgagag tgataagaaa tttttcgcca
     7081 agctgacctc agtgctgaat acaatcctgc agatgcggaa ctcaaagacc gggacagaac
     7141 tggactatct gattagcccc gtggctgatg tcaacggaaa cttcttcgac agcagacagg
     7201 cacccaaaaa tatgcctcag gatgcagccg ccaacggggc ctaccacatc gggctgaagg
     7261 gactgatgct gctgggccgg atcaagaaca atcaggaggg gaagaagctg aacctggtca
     7321 ttaagaacga ggaatacttc gagtttgtcc agaatagaaa taacagtcta gaggcgctac
     7381 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     7441 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     7501 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     7561 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     7621 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     7681 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     7741 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7801 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     7861 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     7921 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     7981 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     8041 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     8101 tgtacaagtc gaattctaag ccaggattcc atatgaagcg acctgccgcc acaaagaagg
     8161 ctggacaggc taagaagaag aaacaacgcg tgcagctaag cgactacaaa gaccatgacg
     8221 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagtaa ggatccctcc
     8281 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     8341 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     8401 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     8461 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     8521 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     8581 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     8641 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     8701 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     8761 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     8821 ctttgaaaaa cacgatgata aggatccacc ggagcttacc atgaccgagt acaagcccac
     8881 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8941 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9001 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9061 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9121 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9181 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggctac
     9241 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9301 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9361 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9421 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccatcga tagatcctaa
     9481 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     9541 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     9601 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     9661 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     9721 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     9781 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     9841 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     9901 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     9961 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    10021 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
    10081 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    10141 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    10201 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10261 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10321 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
    10381 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
    10441 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
    10501 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    10561 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10621 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10681 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    10741 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    10801 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    10861 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    10921 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10981 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    11041 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    11101 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    11161 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11221 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    11281 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    11341 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    11401 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    11461 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    11521 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    11581 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    11641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11701 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11761 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11821 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11881 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11941 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    12001 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    12061 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    12121 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    12181 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    12241 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    12301 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    12361 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    12421 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    12481 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    12541 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    12601 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    12661 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    12721 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    12781 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
    12841 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12901 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    12961 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    13021 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    13081 cttagtagt
//
