LOCUS       sequence-478706-       10589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HK2 lentiviral overexpression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10589)
  TITLE     Cantor Lab lentiviral overexpression vector
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLJC6-HK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2881..4092
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3929..3948
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     RBS             6584..6592
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6900..6965
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7013..7130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7179..7689
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7608..7626
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7698..8093
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8165..8398
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8470..8591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8507..8526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8561..8580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8631..8766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8786..8805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8787..8805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8815..8832)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8815..8831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8824..8846)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8973..9428
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9060..9079)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9270..9291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9454..9558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9559..9627,9628..10419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcgggatct
     2881 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
     2941 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
     3001 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
     3061 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
     3121 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
     3181 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
     3241 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
     3301 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
     3361 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
     3421 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
     3481 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     3541 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3601 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     3661 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     3721 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3781 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3841 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     3901 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     3961 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     4021 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     4081 tttttgttag acgaagcgct agcgctaccg gtttaattaa cggcaggatg attgcctcgc
     4141 atctgcttgc ctacttcttc acggagctca accatgacca agtgcagaag gttgaccagt
     4201 atctctacca catgcgcctc tctgatgaga ccctcttgga gatctctaag cggttccgca
     4261 aggagatgga gaaagggctt ggagccacca ctcaccctac tgcagcagtg aagatgctgc
     4321 ccacctttgt gaggtccact ccagatggga cagaacacgg agagttcctg gctctggatc
     4381 ttggagggac caacttccgt gtgctttggg tgaaagtaac ggacaatggg ctccagaagg
     4441 tggagatgga gaatcagatc tatgccatcc ctgaggacat catgcgaggc agtggcaccc
     4501 agctgtttga ccatatcgcc gagtgtctgg caaacttcat ggataagcta caaatcaaag
     4561 acaagaagct cccactgggt tttaccttct cgttcccctg ccaccagact aaactagacg
     4621 agagtttcct ggtctcatgg accaagggat tcaagtccag tggagtggaa ggcagagacg
     4681 ttgtggctct gatccggaag gccatccaga ggagagggga ctttgatatc gacattgtgg
     4741 ctgtggtgaa tgacacagtt gggaccatga tgacctgtgg ttatgatgac cacaactgtg
     4801 agattggtct cattgtgggc acgggcagca acgcctgcta catggaagag atgcgccaca
     4861 tcgacatggt ggaaggcgat gaggggcgga tgtgcatcaa tatggagtgg ggggccttcg
     4921 gggacgatgg ctcgctcaac gacattcgca ctgagtttga ccaggagatt gacatgggct
     4981 cactgaaccc gggaaagcaa ctgtttgaga agatgatcag tgggatgtac atgggggagc
     5041 tggtgaggct tatcctggtg aagatggcca aggaggagct gctctttggg gggaagctca
     5101 gcccagagct tctcaacacc ggtcgctttg agaccaaaga catctcagac attgaagggg
     5161 agaaggatgg catccggaag gcccgtgagg tcctgatgcg gttgggcctg gacccgactc
     5221 aggaggactg cgtggccact caccggatct gccagatcgt gtccacacgc tccgccagcc
     5281 tgtgcgcagc caccctggcc gccgtgctgc agcgcatcaa ggagaacaaa ggcgaggagc
     5341 ggctgcgctc tactattggg gtcgacggtt ccgtctacaa gaaacacccc cattttgcca
     5401 agcgtctaca taagaccgtg cggcggctgg tgcccggctg cgatgtccgc ttcctccgct
     5461 ccgaggatgg cagtggcaaa ggtgcagcca tggtgacagc agtggcttac cggctggccg
     5521 atcaacaccg tgcccgccag aagacattag agcatctgca gctgagccat gaccagctgc
     5581 tggaggtcaa gaggaggatg aaggtagaaa tggagcgagg tctgagcaag gagactcatg
     5641 ccagtgcccc cgtcaagatg ctgcccacct acgtgtgtgc taccccggac ggcacagaga
     5701 aaggggactt cttggccttg gaccttggag gaacaaattt ccgggtcctg ctggtccgtg
     5761 ttcggaatgg gaagtggggt ggagtggaga tgcacaacaa gatctacgcc atcccgcagg
     5821 aggtcatgca cggcaccggg gacgagctct ttgaccacat tgtccagtgc atcgcggact
     5881 tcctcgagta catgggcatg aagggcgtgt ccctgcctct gggttttacc ttctccttcc
     5941 cctgccagca gaacagcctg gacgagagca tcctcctcaa gtggacaaaa ggcttcaagg
     6001 catctggctg cgagggcgag gacgtggtga ccctgctgaa ggaagcgatc caccggcgag
     6061 aggagtttga cctggatgtg gttgctgtgg tgaacgacac agtcggaact atgatgacct
     6121 gtggctttga agaccctcac tgtgaagttg gcctcattgt tggcacgggc agcaatgcct
     6181 gctacatgga ggagatgcgc aatgtggaac tggtggaagg agaagagggg cggatgtgtg
     6241 tgaacatgga atggggggcc ttcggggaca atggatgcct agatgacttc cgcacagaat
     6301 ttgatgtggc tgtggatgag ctttcactca acccgggcaa gcagaggttc gagaaaatga
     6361 tcagtggaat gtacctgggt gagattgtcc gtaacattct catcgatttc accaagcgtg
     6421 gactactctt ccgaggccgc atctcagagc ggctcaagac aaggggcatc tttgaaacca
     6481 agttcttgtc tcagattgag agtgactgcc tggccctgct gcaagtccga gccatcctgc
     6541 aacacttagg gcttgagagc acctgtgacg acagcatcat tgttaaggag gtgtgcactg
     6601 tggtggcccg gcgggcagcc cagctctgtg gcgcaggcat ggccgctgtg gtggacagga
     6661 tacgagaaaa ccgtgggctg gacgctctca aagtgacagt gggtgtggat gggaccctct
     6721 acaagctaca tcctcacttt gccaaagtca tgcatgagac agtgaaggac ctggctccga
     6781 aatgtgatgt gtctttcctg cagtcagagg atggcagcgg gaagggggcg gcgctcatca
     6841 ctgctgtggc ctgccgcatc cgtgaggctg gacagcgagg aggaagtgcg gccgcaggcg
     6901 actacaaaga ccatgacggt gattataaag atcatgacat tgattacaag gatgacgatg
     6961 acaagtaata agaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
     7021 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     7081 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     7141 acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc
     7201 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     7261 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     7321 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     7381 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     7441 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     7501 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     7561 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     7621 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     7681 tctccccagg gggatccatg gttcctttgt ctcaagaaga atccaccctc attgaaagag
     7741 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     7801 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     7861 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     7921 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     7981 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     8041 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taaggtacct
     8101 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8161 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8221 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8281 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8341 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8401 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8461 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8521 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8581 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     8641 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8701 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8761 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     8821 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8881 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     8941 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     9001 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9061 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9121 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9181 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9241 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9301 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9361 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     9421 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9781 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    10021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10081 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    10261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    10321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    10381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10561 gaccccgtag aaaagatcaa aggatcttc
//
