LOCUS       sequence-478708-       10538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HK2 lentiviral overexpression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10538)
  TITLE     Cantor Lab lentiviral overexpression vector
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLJC6-HK2 (delta MBD)-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2881..4092
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3929..3948
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     RBS             6533..6541
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6849..6914
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6962..7079
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7128..7638
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7557..7575
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7647..8042
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8114..8347
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8419..8540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8456..8475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8510..8529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8580..8715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8735..8754)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8736..8754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8764..8781)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8764..8780)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8773..8795)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8922..9377
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9009..9028)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9219..9240
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9403..9507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9508..9576,9577..10368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcgggatct
     2881 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
     2941 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
     3001 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
     3061 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
     3121 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
     3181 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
     3241 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
     3301 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
     3361 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
     3421 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
     3481 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     3541 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3601 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     3661 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     3721 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3781 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3841 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     3901 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     3961 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     4021 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     4081 tttttgttag acgaagcgct agcgctaccg gtttaattaa cggcaggatg ggaggcagcg
     4141 ttgaccagta tctctaccac atgcgcctct ctgatgagac cctcttggag atctctaagc
     4201 ggttccgcaa ggagatggag aaagggcttg gagccaccac tcaccctact gcagcagtga
     4261 agatgctgcc cacctttgtg aggtccactc cagatgggac agaacacgga gagttcctgg
     4321 ctctggatct tggagggacc aacttccgtg tgctttgggt gaaagtaacg gacaatgggc
     4381 tccagaaggt ggagatggag aatcagatct atgccatccc tgaggacatc atgcgaggca
     4441 gtggcaccca gctgtttgac catatcgccg agtgtctggc aaacttcatg gataagctac
     4501 aaatcaaaga caagaagctc ccactgggtt ttaccttctc gttcccctgc caccagacta
     4561 aactagacga gagtttcctg gtctcatgga ccaagggatt caagtccagt ggagtggaag
     4621 gcagagacgt tgtggctctg atccggaagg ccatccagag gagaggggac tttgatatcg
     4681 acattgtggc tgtggtgaat gacacagttg ggaccatgat gacctgtggt tatgatgacc
     4741 acaactgtga gattggtctc attgtgggca cgggcagcaa cgcctgctac atggaagaga
     4801 tgcgccacat cgacatggtg gaaggcgatg aggggcggat gtgcatcaat atggagtggg
     4861 gggccttcgg ggacgatggc tcgctcaacg acattcgcac tgagtttgac caggagattg
     4921 acatgggctc actgaacccg ggaaagcaac tgtttgagaa gatgatcagt gggatgtaca
     4981 tgggggagct ggtgaggctt atcctggtga agatggccaa ggaggagctg ctctttgggg
     5041 ggaagctcag cccagagctt ctcaacaccg gtcgctttga gaccaaagac atctcagaca
     5101 ttgaagggga gaaggatggc atccggaagg cccgtgaggt cctgatgcgg ttgggcctgg
     5161 acccgactca ggaggactgc gtggccactc accggatctg ccagatcgtg tccacacgct
     5221 ccgccagcct gtgcgcagcc accctggccg ccgtgctgca gcgcatcaag gagaacaaag
     5281 gcgaggagcg gctgcgctct actattgggg tcgacggttc cgtctacaag aaacaccccc
     5341 attttgccaa gcgtctacat aagaccgtgc ggcggctggt gcccggctgc gatgtccgct
     5401 tcctccgctc cgaggatggc agtggcaaag gtgcagccat ggtgacagca gtggcttacc
     5461 ggctggccga tcaacaccgt gcccgccaga agacattaga gcatctgcag ctgagccatg
     5521 accagctgct ggaggtcaag aggaggatga aggtagaaat ggagcgaggt ctgagcaagg
     5581 agactcatgc cagtgccccc gtcaagatgc tgcccaccta cgtgtgtgct accccggacg
     5641 gcacagagaa aggggacttc ttggccttgg accttggagg aacaaatttc cgggtcctgc
     5701 tggtccgtgt tcggaatggg aagtggggtg gagtggagat gcacaacaag atctacgcca
     5761 tcccgcagga ggtcatgcac ggcaccgggg acgagctctt tgaccacatt gtccagtgca
     5821 tcgcggactt cctcgagtac atgggcatga agggcgtgtc cctgcctctg ggttttacct
     5881 tctccttccc ctgccagcag aacagcctgg acgagagcat cctcctcaag tggacaaaag
     5941 gcttcaaggc atctggctgc gagggcgagg acgtggtgac cctgctgaag gaagcgatcc
     6001 accggcgaga ggagtttgac ctggatgtgg ttgctgtggt gaacgacaca gtcggaacta
     6061 tgatgacctg tggctttgaa gaccctcact gtgaagttgg cctcattgtt ggcacgggca
     6121 gcaatgcctg ctacatggag gagatgcgca atgtggaact ggtggaagga gaagaggggc
     6181 ggatgtgtgt gaacatggaa tggggggcct tcggggacaa tggatgccta gatgacttcc
     6241 gcacagaatt tgatgtggct gtggatgagc tttcactcaa cccgggcaag cagaggttcg
     6301 agaaaatgat cagtggaatg tacctgggtg agattgtccg taacattctc atcgatttca
     6361 ccaagcgtgg actactcttc cgaggccgca tctcagagcg gctcaagaca aggggcatct
     6421 ttgaaaccaa gttcttgtct cagattgaga gtgactgcct ggccctgctg caagtccgag
     6481 ccatcctgca acacttaggg cttgagagca cctgtgacga cagcatcatt gttaaggagg
     6541 tgtgcactgt ggtggcccgg cgggcagccc agctctgtgg cgcaggcatg gccgctgtgg
     6601 tggacaggat acgagaaaac cgtgggctgg acgctctcaa agtgacagtg ggtgtggatg
     6661 ggaccctcta caagctacat cctcactttg ccaaagtcat gcatgagaca gtgaaggacc
     6721 tggctccgaa atgtgatgtg tctttcctgc agtcagagga tggcagcggg aagggggcgg
     6781 cgctcatcac tgctgtggcc tgccgcatcc gtgaggctgg acagcgagga ggaagtgcgg
     6841 ccgcaggcga ctacaaagac catgacggtg attataaaga tcatgacatt gattacaagg
     6901 atgacgatga caagtaataa gaattctcga cctcgagaca aatggcagta ttcatccaca
     6961 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     7021 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     7081 gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg
     7141 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     7201 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     7261 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     7321 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     7381 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     7441 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     7501 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     7561 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     7621 accgacctct ctccccaggg ggatccatgg ttcctttgtc tcaagaagaa tccaccctca
     7681 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     7741 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     7801 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     7861 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     7921 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     7981 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     8041 aaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8101 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     8161 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8221 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8281 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8341 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8401 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8521 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8581 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8641 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8701 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     8761 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     8821 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     8881 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     8941 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9001 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9061 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9121 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9181 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9241 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     9301 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     9361 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9481 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9541 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9601 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9661 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9721 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9781 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9841 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9901 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9961 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10021 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    10081 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10141 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    10201 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    10261 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    10321 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    10381 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10441 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10501 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
