LOCUS       sequence-47891-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     Gary Roberts unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUX19
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(28..44)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(28..44)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(172..188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(172..188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(185..207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    196..212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    267..288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     oriT            528..637
                     /note="incP origin of transfer"
ORIGIN
        1 gactcactat agggcgaatt ggggatcgat ccactagttc tagagcggcc gccacggcga
       61 tatcggatcc atatgacgtc gacgcgtctg cagaagcttc gaattcgagc tcccgggtac
      121 catggcatgc atcgatagat ctcgaggcct cggactagtg gcgtaatcat ggtcatagct
      181 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccgcggaagc
      241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      361 cgcgcgggga gaggcggttt gcgtattggg cgcttttccg ctgcataacc ctgcttcggg
      421 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
      481 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
      541 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
      601 gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag
      661 ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcaaggtg
      721 tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc
      781 ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt cgtggactat
      841 gagcacgtcc gcgagctggc
//
