LOCUS       sequence-47893-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pZW16 (CK2alpha')
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..193
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttccaccat gggccgcatc ttttacccat acgatgttcc
      121 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
      181 tccagattac gctgctcagt gcggccgccc cggcccggcc gcgggcagca gggcccgggt
      241 ctacgccgag gtgaacagtc tgaggagccg cgagtactgg gactacgagg ctcacgtccc
      301 gagctggggt aatcaagatg attaccaact ggttcgaaaa cttggtcggg gaaaatatag
      361 tgaagtattt gaggccatta atatcaccaa caatgagaga gtggttgtaa aaatcctgaa
      421 gccagtgaag aaaaagaaga taaaacgaga ggttaagatt ctggagaacc ttcgtggtgg
      481 aacaaatatc attaagctga ttgacactgt aaaggacccc gtgtcaaaga caccagcttt
      541 ggtatttgaa tatatcaata atacagattt taagcaactc taccagatcc tgacagactt
      601 tgatatccgg ttttatatgt atgaactact taaagctctg gattactgcc acagcaaggg
      661 aatcatgcac agggatgtga aacctcacaa tgtcatgata gatcaccaac agaaaaagct
      721 gcgactgata gattggggtc tggcagaatt ctatcatcct gctcaggagt acaatgttcg
      781 tgtagcctca aggtacttca agggaccaga gctcctcgtg gactatcaga tgtatgatta
      841 tagcttggac atgtggagtt tgggctgtat gttagcaagc atgatctttc gaagggaac
//
