LOCUS       sequence-478983-       13328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to overexpress the NanoLUC gene via 
            virus-mediated overexpression (VOX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVO-NanoLUC
FEATURES             Location/Qualifiers
     source          1..13328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3769..4284
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(6414..6431)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6414..6430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6423..6445)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6633..6657
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7958..8587
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9016..10089
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10155..10349
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10585..10607)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10693..10833
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10748..10767
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10848..10865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11019..11607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11099..11118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11694..12488)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12913..12937
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13205..13226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13241..13246,13247..13264,13265..13271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13279..13295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13284..13306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13303..13319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13303..13319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtat ggtcttcaca
     3781 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
     3841 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
     3901 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
     3961 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     4021 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     4081 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     4141 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     4201 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     4261 ctgtgcgaac gcattctggc gtaaattcgc tagcgttaac tggccataat cctgtgtatg
     4321 caacgttgaa aattcggatc tatttttacg attcgatcat gaattaataa aatttaaatt
     4381 ttattgaatg attttctagt acataactga catacgactt atcagttgcg aaacgaacag
     4441 ctctaattac attgttaagt gaaattacgc ctaattcgtc taagtacaac ataaccaact
     4501 gtttaaacct aattaaataa gtcgacccag aagctgtcag agatgtcgtc cagacttgga
     4561 agttcaggta agctttgtgg agatgcaacg ctctccgtaa gttgtggttg aatcggatct
     4621 ggacgtggta tatacgcgtc ctggtgtata gcagatcctc tactccgtat atcttgaaat
     4681 ataggggatt tattatctcc cagatataca cgccattctc tgcctgacgt gcagtgatga
     4741 tttcccctgt gcgtgaatcc atgaccggtg cagcctatgt ggaagtatat ggtgcaccca
     4801 cagtttagat caatgcgtcg tcgtctaatc gccctcctct tggcttgcct gtgtgccttc
     4861 ttgatagagg ggggcgtcga gagtgatgaa gataacattc ttgagagtcc aatgtttgag
     4921 cgacgcattt tccgctttat ccaggaaatc tttatagctg gatccctcgc ctggattgca
     4981 aagcacgatt gaagggatcc ctcctttaat ttgaactggt ttcccgtact tgcaatttga
     5041 ctgccagtct ttttgggccc caagaagctc tttccaatgc tttagcttta ggtattgcgg
     5101 tgctacgtca tcaatgacgt tataggcaac attattcgag tacactcgat tattgaagtc
     5161 caggtgtcca ctgaggtaat tatgtgggcc taaagcacga gcccacatcg tcttgcccgt
     5221 tctagaatca ccttccacta tgagactaac tggtcttaca ggccgcgcag cgggacccat
     5281 tccaaaataa tcatccgccc attcttgcat ctgttcagga acgttagtga aagaggaaag
     5341 tggaaatgga gggacccatt gttcaggcgc ctttttgaaa aggcgttcca agttcgctgt
     5401 aatgttatgg taactcacga tgaacgccct tggatctccg gcccggataa ttgcaagagc
     5461 ttccgccaaa ctaggtgcat tgatggcgtt ataatagacg tcgtctttat tcttctttgt
     5521 acccccaggc actttgaact gtccggattc acaacaatcg ccgtctttgg tgatgtaatt
     5581 cttgacggcg ttggcgtctt tggctgcttg tatgtttggg tgaaaattgg tcgaccttct
     5641 ggggtgagta aggtcgaaaa atctcttatt cttgatgttg gactttccag agagttggat
     5701 gagacagtga aggtgtggga atccgtccga gtgctcctct ctggcaactc gaatatatgt
     5761 tggtttgacg actgaccatg ataaggtttg aagcatttga agagcttcat tttttgggat
     5821 atcgcacttg ggataggtta agaaaatatg tctggcttgt aaacgaaaag aatcggggtt
     5881 tctaggcatt ttttaaatag tgacccagga ctcaaaaact agctctcaac ttctctcata
     5941 tgttgtggag tctggagtcc tttatatatg tttacgctat agaaggcatt tggggactct
     6001 cccgggccca ccataggact ccataccgcg gccatctata taatattact gatggccgcg
     6061 cggattttgg agcccccttt tgtcgttagc gcgaccttta attcaaatta aaggttgctt
     6121 ttttctatcg tccaatcatt ttgcgcctga ctagcctaga tattagcaac aacttcgcga
     6181 ctaagttgtt gaccgtcaag tataaattaa tgggcttatg gcccaatggg tatagagcaa
     6241 aatgcccaag cgtgatgccc cgtggcgctt attagcgggt acgtctaagg taagtcgaaa
     6301 tgccaactac tctcccttga gagtattggg ccctaaagtt aataagggac ctcgaggggg
     6361 ggcccggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
     6421 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6481 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6541 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc
     6601 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     6661 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     6721 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     6781 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     6841 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     6901 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     6961 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     7021 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     7081 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     7141 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     7201 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     7261 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     7321 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     7381 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     7441 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     7501 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     7561 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     7621 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     7681 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     7741 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     7801 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     7861 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     7921 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     7981 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     8041 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     8101 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     8161 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     8221 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     8281 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     8341 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     8401 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     8461 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     8521 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     8581 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     8641 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     8701 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     8761 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     8821 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     8881 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     8941 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
     9001 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     9061 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     9121 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     9181 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     9241 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     9301 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     9361 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     9421 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     9481 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     9541 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     9601 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     9661 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     9721 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     9781 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     9841 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     9901 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     9961 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    10021 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    10081 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    10141 aaaggtgtct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    10201 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    10261 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    10321 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
    10381 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    10441 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    10501 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    10561 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10621 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10681 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
    10741 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    10801 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
    10861 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    10921 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    10981 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    11041 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    11101 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    11161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    11221 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    11281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    11341 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    11401 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    11461 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    11521 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    11581 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    11641 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
    11701 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    11761 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    11821 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    11881 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    11941 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    12001 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    12061 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    12121 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    12181 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    12241 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    12301 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    12361 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    12421 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    12481 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    12541 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    12601 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    12661 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    12721 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    12781 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    12841 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    12901 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    12961 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc ggggatagcc
    13021 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    13081 accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga
    13141 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    13201 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    13261 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    13321 tgattacg
//
