LOCUS       sequence-47901-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L, Helenius A
  TITLE     Assembly and trafficking of caveolar domains in the cell: caveolae 
            as stable, cargo-triggered, vesicular transporters.
  JOURNAL   J Cell Biol. 2005 Aug 29. 170(5):769-79.
  PUBMED    16129785
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2005 Aug 29. 170(5):769-79."
COMMENT     Sequence Label: Cav1-mRFP
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      148..157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(783..834)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(812..832)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
       61 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga
      121 actccttgat gacgtcctcg gaggaggcca tggtggcgac cggccggtgg atccctgttt
      181 ctttctgcat gttgatgcgg atattgctga atattttgcc aacagcctca aagaacgggt
      241 cacagaaggt gtggacgtag atggaataga cacggctgat gcactgaatc tcaatcagga
      301 aactcttaat gcacggcaca actgcccaga tgtgcaggaa agaaagaatg gcaaagtaaa
      361 tgccccatat gagtgccatt gggatgccaa agagggcaga cagcaagcgg taaaaccagt
      421 attttgtcac agtgaaggtg gtgaagctgg ccttccagat gccatcaaaa ctgtgtgttc
      481 cttctggttc tgcaatcaca tcttcaaaat caatcttgac cacgtcgtcg ttgagatgct
      541 tggggtcgcg gttgaccagg tcgatttcct tggtgtgcgc gtcgtacacc tgcttctcgc
      601 tcatctcctc cgccatggcc ttgttgttgg gcttgtagat gttgccctgc tcccggatgg
      661 gaacggtgta gaggtgcccc tcggagtcta cgtatttgcc cccagacatc caagccgaat
      721 tcgaagcttg agctcgagat ctgagtccgg tagcgctagc ggatctgacg gttcactaaa
      781 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      841 gcggagttgt t
//
