LOCUS       sequence-47911-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA
  TITLE     Single action potentials and subthreshold electrical events imaged 
            in neurons with a fluorescent protein voltage probe.
  JOURNAL   Neuron. 2012 Sep 6;75(5):779-85.
  PUBMED    22958819
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2012-09-6"; volume: "75(5)"; pages: "779-85"
COMMENT     Sequence Label: ArcLight-S249
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(850..878)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgccattctg cctggggacg tcggagctag cttgatttag gtgacactat agaatacaag
       61 cttatggagg gattcgacgg ttcagatttt agtcctccag ctgatttagt tggcgttgac
      121 ggtgcagtca tgcggaacgt cgttgacgtc acgataaatg gtgacgtcac tgctccgccg
      181 aaagctgcgc caagaaaaag tgaatcggta aagaaagttc attggaatga tgtagaccaa
      241 ggaccgagtg aaaaaccaga gacaagacag gaggaacgaa tagatatacc cgagatttca
      301 ggtctatggt ggggcgagaa tgaacatgga gtggacgatg ggagaatgga gatacctact
      361 actggtgtag gtcgcgtcca gtttcgtgtc cgagcagtga ttgatcatct agggatgcga
      421 gtctttggag tcttcctaat tttcttggac atcatcctca tgatcattga tctcagtctt
      481 ccaggaaaaa gtgaatcttc acaatccttt tatgacggga tggctttggc tctttcttgt
      541 tatttcatgc tggatttagg attaaggata tttgcctacg ggcccaagaa tttcttcacc
      601 aacccctggg aggttgctga tggtttgatt atcgttgtca cattcgtcgt cacgatattt
      661 tacactgtgt tagatgaata cgtgcaagaa acaggagccg atggtttggg gcaattggtt
      721 gtgttggccc gtttgctgcg tgtggttcga ttagcaagaa tattttattc ccaccaacaa
      781 atgaaggctt caagcagaag aacaatatca ggggatccca tgagtaaagg agaagaactt
      841 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaa
//
