LOCUS       sequence-479120-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses trypansoma brucei 6 phosphogluconate dehydrogenase under 
            T7 with HIS tag in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  TITLE     Josh Beckham Lab plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TB6PDGH-pNIC-Bsa4
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             34..56
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             109..129
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 annnnnnnng nnnnnntnnc ccnctagaat aattttgttt aactttaaga aggagatata
       61 catatgcacc atcatcatca ttattcttct ggtgtagatc tgggtaccga gaacctgtac
      121 ttccaatcca tgtctatgga cgttggtgta gttggcctgg gtgttatggg tgcgaacctg
      181 gcgctgaata ttgcggaaaa aggtttcaaa gttgcggttt tcaaccgtac ctactctaaa
      241 tctgaagaat ttatgaaagc gaacgcttct gcaccgtttg cgggcaacct gaaggcgttt
      301 gagaccatgg aagcatttgc ggcgtctctc aagaaaccgc gtaaagcgct cattctggta
      361 caagcgggtg cggccaccga ctctaccatc gaacagctca aaaaagtatt tgaaaagggt
      421 gacatcctgg tcgacaccgg taacgcgcat ttcaaggacc agggtcgtcg tgcgcagcag
      481 ctggaggctg cgggtctccg tttcctcggc atgggtattt ctggtggtga agaaggtgcg
      541 cgtaagggtc cggcgttctt cccgggtggt accctgtctg tatgggaaga gattcgtccg
      601 atcgttgaag ctgccgctgc gaaagcggac gatggtcgcc cgtgcgtaac gatgaatggt
      661 tccggtggtg ctggttcttg cgttaaaatg taccacaaca gcggtgaata cgcgatcctg
      721 caaatctggg gtgaagtatt cgatatcctg cgcgcgatgg gcctgaataa tgacgaagta
      781 gccgcggttc tggaagactg gaaaagcaaa aactttctca aatcctatat gctggacatc
      841 tccatcgctg cggcacgcgc taaagacaag gacggctcct acctgaccga gcacgtgatg
      901 gaccgtatcg gtagcaaagg taccggtctg tggtctgccc aggaagcgct ggaaatcggt
      961 gtacctgcgc catccctgaa catggcggta gtttcccgcc aattcacatg tacaagaccg
     1021 aacgtcaggc gaatgcgtct aacgctccgg gatcacccag tctcctggta acacctgaaa
     1081 aacaatctcc gtctggcccg aaatcaaaaa ctgtacgatt ttgttgnttg ttattatttt
     1141 tgctactcca atgttcaatg ctgccggaaa
//
