LOCUS       sequence-479265-        3098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp7
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(887..1678)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2109..2125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2109..2125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2130..2149
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2130..2148
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2166..2265
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2574..2673)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2689..2706)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2689..2705)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2698..2720)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2769..2855
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2947..2974
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 acgcgtaact cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta
      901 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
      961 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
     1021 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     1081 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     1141 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     1201 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     1261 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     1321 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     1381 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     1441 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     1501 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
     1561 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     1621 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa
     1681 tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca
     1741 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
     1801 ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     1861 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac
     1921 gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     1981 acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagga
     2041 ggcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc
     2101 agagctgcca ggaaacagct atgaccatgt aatacgactc actatagggg atatcagctg
     2161 gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
     2221 agcaattatt ttttataatg ccaactttgt acaagaaagc tgggcccatg aggccgcgat
     2281 cgcgtcttaa ttaagttgaa gtacgctagg agtgtcaaaa atatcactag ctaacgcatc
     2341 aagatctaca ttaccagaaa aagtcattaa agtagcaaag agacttacga atttctcgaa
     2401 agcctcactg gggtctgttg ctgccaatat atcattatgg catttaacac agaaagccca
     2461 ggccctacta ttagcctcta agtgtaactg ttggagcaca gagaggagaa ccacagatgt
     2521 gcatttaaga tctgtaagtt tagaggatcc catggatcct ggccttgacg gcccttctgc
     2581 ttttttgtac aaagttggca ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca
     2641 ggtcactatc agtcaaaata aaatcattat ttggggcccg agcttaagac tggccgtcgt
     2701 tttacaacgt cgtgactggg aaaacatcca tgctagcgtt aacgcgagag tagggaactg
     2761 ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt
     2821 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     2881 tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     2941 ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctggct
     3001 agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3061 gtgccagctg cattaatgaa tcggccaacg cgcgggga
//
