LOCUS       sequence-479269-        3173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-ORF4a
FEATURES             Location/Qualifiers
     source          1..3173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     431..448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(607..634)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(726..812)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     861..883
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     875..892
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     876..892
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    908..1007
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1391..1490)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1507..1526)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1508..1526)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1531..1547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1531..1547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1978..2769
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2862..3173,1..277)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
       61 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      301 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      361 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      421 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      481 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      541 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
      601 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      661 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      721 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      781 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
      841 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
      901 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      961 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcagaagggc cgtcaaggcc
     1021 aggatccatg ggatccgatt acgtgtctct gcttaatcaa atttggcaga agtaccttaa
     1081 ctcaccgtat actacttgtt tgtacatccc taaacccaca gctaagtata cacctttagt
     1141 tggcacttca ttgcaccctg tgctgtggaa ctgtcagcta tcctttgctg gttatactga
     1201 atctgctgtt aattctacaa aagctttggc caaacaggac gcagctcagc gaatcgcttg
     1261 gttgctacat aaggatggag gaatccctga tggatgttcc ctctacctcc ggcactcaag
     1321 tttattcgcg caaagcgagg aagaggagcc attctccaac cttaattaag acgcgatcgc
     1381 ggcctcatgg gcccagcttt cttgtacaaa gttggcatta taaaaaataa ttgctcatca
     1441 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1501 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1561 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     1621 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     1681 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     1741 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1801 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     1861 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1921 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     1981 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     2041 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2101 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2161 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2221 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2281 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2341 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2401 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2461 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2521 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2581 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2641 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2701 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     2761 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     2821 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2881 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2941 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3001 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3061 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3121 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtc
//
