LOCUS       sequence-479272-        4829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp2
FEATURES             Location/Qualifiers
     source          1..4829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             132..923
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1016..1604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1505..1524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1758..1775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1934..1961)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2053..2139)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2188..2210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2202..2219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2203..2219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2235..2334
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4374..4473)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4490..4509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4491..4509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4514..4530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4514..4530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
       61 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
      121 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
      181 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
      241 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
      301 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
      361 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
      421 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
      481 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
      541 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
      601 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
      661 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
      721 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
      781 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
      841 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
      901 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
      961 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1021 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1081 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1141 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1201 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1261 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1321 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1381 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1441 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1501 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1561 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1621 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1681 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1741 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     1801 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1861 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     1921 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     1981 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     2041 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     2101 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     2161 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     2221 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     2281 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca gaagggccgt
     2341 caaggccagg atccatggga tccgatgtca ctccagttga ccaatacatg tgtggcgttg
     2401 atggaaaacc cattagtgcc tacgcatttt taatggccaa ggatggaata accaaactgg
     2461 ctgatgttga agcggacgtc gcagcacgtg ctgatgacga aggcttcatc acattaaaga
     2521 acaatctata tagattggtt tggcatgttg agcgtaaaga cgttccatat cctaagcaat
     2581 ctatttttac tattaatagt gtggtccaaa aggatggtgt tgaaaacact cctcctcact
     2641 attttactct tggatgcaaa attttaacgc tcaccccacg caacaagtgg agtggcgttt
     2701 ctgacttgtc cctcaaacaa aaactccttt acaccttcta tggtaaggag tcacttgaga
     2761 acccaaccta catttaccac tccgcattca ttgagtgtgg aagttgtggt aatgattcct
     2821 ggcttacagg gaatgctatc caagggtttg cctgtggatg tggggcatca tatacagcta
     2881 atgatgtcga agtccaatca tctggcatga ttaagccaaa tgctcttctt tgtgctactt
     2941 gcccctttgc taagggtgat agctgttctt ctaattgcaa acattcagtt gctcagttgg
     3001 ttagttacct ttctgaacgc tgtaatgtta ttgctgattc taagtccttc acacttatct
     3061 ttggtggcgt agcttacgcc tactttggat gtgaggaagg tactatgtac tttgtgccta
     3121 gagctaagtc tgttgtctca aggattggag actccatctt tacaggctgt actggctctt
     3181 ggaacaaggt cactcaaatt gctaacatgt tcttggaaca gactcagcat tcccttaact
     3241 ttgtgggaga gttcgttgtc aacgatgttg tcctcgcaat tctctctgga accacaacta
     3301 atgttgacaa aatacgccag cttctcaaag gtgtcaccct tgacaagttg cgtgattatt
     3361 tagctgacta tgacgtagca gtcactgccg gcccattcat ggataatgct attaatgttg
     3421 gtggtacagg attacagtat gccgccatta ctgcacctta tgtagttctc actggcttag
     3481 gtgagtcctt taagaaagtt gcaaccatac cgtataaggt ttgcaactct gttaaggata
     3541 ctctggctta ttatgctcac agcgtgttgt acagagtttt tccttatgac atggattctg
     3601 gtgtgtcatc ctttagtgaa ctactttttg attgcgttga tctttcagta gcttctacct
     3661 attttttagt ccgcatcttg caagataaga ctggcgactt tatgtctaca attattactt
     3721 cctgccaaac tgctgttagt aagcttctag atacatgttt tgaagctaca gaagcaacat
     3781 ttaacttctt gttagatttg gcaggattgt tcagaatctt tctccgcaat gcctatgtgt
     3841 acacttcaca agggtttgtg gtggtcaatg gcaaagtttc tacacttgtc aaacaagtgt
     3901 tagacttgct taataagggt atgcaacttt tgcatacaaa ggtctcctgg gctggttcta
     3961 aaatcattgc tgttatctac agcggcaggg agtctctaat attcccatcg ggaacctatt
     4021 actgtgtcac cactaaggct aagtccgttc aacaagatct tgacgttatt ttgcctggtg
     4081 agttttccaa gaagcagtta ggactgctcc aacctactga caattctaca actgttagtg
     4141 ttactgtatc cagtaacatg gttgaaactg ttgtgggtca acttgagcaa actaatatgc
     4201 atagtcctga tgttatagta ggtgactatg tcattattag tgaaaaattg tttgtgcgta
     4261 gtaaggaaga agacggattt gccttctacc ctgcttgcac taatggtcat gctgtaccga
     4321 ctctctttag acttaaggga ggtcttaatt aagacgcgat cgcggcctca tgggcccagc
     4381 tttcttgtac aaagttggca ttataaaaaa taattgctca tcaatttgtt gcaacgaaca
     4441 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     4501 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     4561 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     4621 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
     4681 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     4741 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     4801 ggcggttttc atggcttgtt atgactgtt
//
