LOCUS       sequence-479278-        3188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp9
FEATURES             Location/Qualifiers
     source          1..3188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             448..1239
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1332..1920
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1821..1840
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2074..2091
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2250..2277)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2369..2455)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2504..2526
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2518..2535
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2519..2535
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2551..2650
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3049..3148)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3165..3184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3166..3184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
       61 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
      121 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
      181 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
      241 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
      301 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
      361 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
      421 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
      481 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
      541 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
      601 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
      661 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
      721 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
      781 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
      841 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
      901 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
      961 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     1021 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     1081 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     1141 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     1201 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     1261 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     1321 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1381 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1441 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1501 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1561 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1621 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1681 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1741 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1801 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1861 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1921 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1981 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2041 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2101 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2161 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     2221 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     2281 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     2341 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     2401 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     2461 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     2521 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
     2581 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
     2641 aaagcagaag ggccgtcaag gccaggatcc atgggatcca ataatgaact gagtccagta
     2701 gcactacgac agatgtcctg tgcggctggt accacacaaa cagcttgtac tgatgacaat
     2761 gcacttgcct actataacaa ttcgaaggga ggtaggtttg tgctggcatt actatcagac
     2821 caccaagatc tcaaatgggc tagattccct aagagtgatg gtacaggtac aatttacaca
     2881 gaactggaac caccttgtag gtttgttaca gacacaccaa aagggcctaa agtgaaatac
     2941 ttgtacttca tcaaaggctt aaacaaccta aatagaggta tggtgctggg cagtttagct
     3001 gctacagtac gtcttcagct taattaagac gcgatcgcgg cctcatgggc ccagctttct
     3061 tgtacaaagt tggcattata aaaaataatt gctcatcaat ttgttgcaac gaacaggtca
     3121 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     3181 attacatg
//
