LOCUS       sequence-479285-        3887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-nsp15
FEATURES             Location/Qualifiers
     source          1..3887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     695..711
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     695..711
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     716..735
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        716..734
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    752..851
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1949..2048)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2064..2081)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2064..2080)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2073..2095)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2144..2230
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2322..2349
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2508..2525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2679..3267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2759..2778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3360..3887,1..264))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
       61 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
      121 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
      181 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
      241 ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
      301 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
      361 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
      421 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
      481 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
      541 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
      601 atttctgtcc tggctggtct agaggaggca tgatgatata tttttatctt gtgcaatgta
      661 acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata
      721 cgactcacta taggggatat cagctggatg gcaaataatg attttatttt gactgatagt
      781 gacctgttcg ttgcaacaaa ttgatgagca attatttttt ataatgccaa ctttgtacaa
      841 gaaagctggg cccatgaggc cgcgatcgcg tcttaattaa gttgtaattt tgggtaaaat
      901 gtttctacat ggccatcttt acaccaaagc ataaatgaaa tttctgtata gtcaatagtc
      961 actttgacaa ccttagaaac tacagataaa tcttgggatt ttattatttc aacaaaatca
     1021 tcaagtaata aatcaataac agaacacaca cacttagatg aacctgtttg cgcatctgtt
     1081 atgaaatagt ttttaactgt actgtccata ggaataaaat cttctaattc aaaaggtgat
     1141 tccttaaaac gtttagctag tccaatcagt agatgtaaac cacctaactg actatgacta
     1201 aaatctccat aaacgatatg ttcgaaggca tagccttcta atttataccg ttcaatgaat
     1261 tcatccatag ctaattctaa gaaatcaatt tccatttgac tcctgggttt aaattcttgt
     1321 aaatttctac tctgagtaaa gtaagtttca ggtaattgtt ggacaacacc atcaactttc
     1381 ttataataat tgaactgtgt ttttacggct tctccaatta atgtgactcc attaagacta
     1441 gcttgtttgg gacctacaga tggttgtaaa cctttaacac taccttctgt aataagaaca
     1501 ccattacggg catttctaaa taagtctact tgaccatcaa ctctaccatc aaaaaagaca
     1561 gtgagtggtg cacaaatcgt ttcagttggt ttcttggcta tgtcagtcat agaacaaaca
     1621 ccaatagtag atatatgtgc tggagcatct cttttgtagt cccagatcac agtattagca
     1681 gcaatgtcca cacccaaatt attgagtatt ttcacctctg gtactggttt aatgttgcgc
     1741 ttagcccaaa gctcaaatgc tacattaaca ggtaatgttg ttttattttc aaacaattct
     1801 acatcaacac catcaacttt tgtgtaaaca gtgttattaa tgatagaaac tggtacttca
     1861 ccctgttgtc catcaaagtg tcccttattt acaacattaa aagccacatt ttctaaactg
     1921 gatcccatgg atcctggcct tgacggccct tctgcttttt tgtacaaagt tggcattata
     1981 aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     2041 attatttggg gcccgagctt aagactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2101 atccatgcta gcgttaacgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     2161 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     2221 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
     2281 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
     2341 tggccttttt gcgtttctac aaactcttcc tggctagcgg tacgcgtatt aattgcgttg
     2401 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2461 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2521 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2581 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2641 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2701 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2761 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2821 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2881 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2941 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3001 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3061 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3121 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3181 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3241 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3301 gctcagtgga acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgagggt
     3361 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     3421 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     3481 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     3541 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     3601 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     3661 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     3721 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     3781 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     3841 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggt
//
