LOCUS       sequence-479298-        4658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-HKU1-nsp13
FEATURES             Location/Qualifiers
     source          1..4658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     381..398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(557..584)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(676..762)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     811..833
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     825..842
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     826..842
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    858..957
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2826..2925)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2942..2961)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2943..2961)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2966..2982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2966..2982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3413..4204
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4297..4658,1..227)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
       61 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      481 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
      541 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
      601 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
      661 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      721 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
      781 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
      841 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
      901 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcagaagggc
      961 cgtcaaggcc aggatccatg ggatccagtg taggtgcatg cgttgtttgt tcatcacaaa
     1021 cttctttgcg ttgtggcagt tgtatacgta agcctttgtt atgttgtaaa tgttgttatg
     1081 accatgttat ggcaactaat cataaatatg ttttgagtgt ctcaccttac gtttgtaatg
     1141 cacctaactg tgatgtgagt gatgtcacca aattatattt gggcggtatg tcttactatt
     1201 gtgaaaacca taaaccccat tattcattta agttagttat gaatggtatg gtctttggtt
     1261 tgtataaaca atcttgcacg ggttcacctt atatagatga ttttaataag atagctagtt
     1321 gtaaatggac agaagttgat gattatgttc tggcaaatga gtgtattgaa cgtttaaagt
     1381 tatttgctgc agaaactcaa aaggcaactg aagaggcttt taaacaaagc tatgcttctg
     1441 ctaccattca agagattgtt agtgatagag aagttatttt gtgttgggag acaggtaaag
     1501 ttaaaccacc acttaataaa aattatgttt tcacaggcta ccattttact agtactggta
     1561 agacagtttt aggtgagtat gtttttgata aaagtgaatt aactaacggt gtgtattacc
     1621 gcgctacaac tacttataaa ctttctatag gtgatgtttt tgttttaaca tcacattctg
     1681 tagctagttt aagtgcacct acacttgtcc cacaagagaa ctatgctagt ataagatttt
     1741 ctagtgttta tagtgttcca ttggtgtttc aaaataatgt tgctaattat cagcacattg
     1801 gaatgaaacg ttattgcact gttcaaggtc cccctggtac gggaaagtct catcttgcta
     1861 taggtctagc tgtttattac tacacagcac gtgtagttta tactgctgct agtcatgctg
     1921 ctgtagatgc attgtgtgaa aaagcttata agtttttaaa tattaacgat tgtacacgta
     1981 ttattcctgc taaagttcgt gtagattgtt atgataagtt taaaattaat gataccactt
     2041 gtaagtatgt ttttaccaca ataaatgcat taccagagtt ggttacagat attgttgttg
     2101 ttgatgaagt tagtatgctt actaattatg aattgtctgt tataaatgct cgtattaaag
     2161 ctaaacatta tgtatatatt ggagatcctg ctcaattacc tgcaccacgt gtgctgttga
     2221 gcaagggttc tttagaacct aggcacttca attctattac taaaataatg tgttgtttag
     2281 gtcctgatat ctttttggga aattgttata ggtgtcctaa agaaattgta gaaactgttt
     2341 cagcattggt ttatgataat aaactcaagg ctaaaaatga taatagttca ttatgtttta
     2401 aagtatattt taagggacag acaacacatg agagttcaag tgctgtaaat attcaacaga
     2461 tatatctaat tagtaaattt ttaaaagcta atccagtttg gaatagtgct gtttttatta
     2521 gtccttataa tagtcagaat tatgttgcta agcgtgtttt aggtgttcaa acacaaactg
     2581 tagattctgc tcaaggttcg gaatatgatt atgttatata ttcacaaaca gcagaaacag
     2641 cccattctgt taatgttaat cgatttaatg ttgccataac tagagccaag aagggcattt
     2701 tttgtgttat gagtaatatg caattatttg aatctcttaa ttttattact ctacctttag
     2761 ataaaattca aaatcaaact ttacctcgtt tgcatcttaa ttaagacgcg atcgcggcct
     2821 catgggccca gctttcttgt acaaagttgg cattataaaa aataattgct catcaatttg
     2881 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     2941 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     3001 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     3061 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     3121 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     3181 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     3241 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     3301 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     3361 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     3421 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     3481 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     3541 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     3601 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     3661 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     3721 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     3781 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     3841 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3901 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     3961 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     4021 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     4081 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     4141 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     4201 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     4261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4621 gggttcgtgc acacagccca gcttggagcg aacgacct
//
