LOCUS       sequence-479304-        4403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-229E-nsp14
FEATURES             Location/Qualifiers
     source          1..4403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             362..1153
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1246..1834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1735..1754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1988..2005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2164..2191)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2283..2369)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2418..2440
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2432..2449
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2433..2449
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2465..2564
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4178..4277)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4294..4313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4295..4313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4318..4334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4318..4334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
       61 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
      121 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      181 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
      241 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
      301 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
      361 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
      421 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
      481 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
      541 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
      601 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
      661 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
      721 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
      781 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
      841 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
      901 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
      961 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     1021 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     1081 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     1141 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     1201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1381 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1921 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1981 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2041 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2101 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
     2161 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
     2221 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
     2281 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     2341 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
     2401 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
     2461 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
     2521 gcaatgcttt tttataatgc caactttgta caaaaaagca gaagggccgt caaggccagg
     2581 atccatggga tcctctgaaa gtagttgtgg tttgtttaag gattgtgcac gtaaccctat
     2641 tgatttacca ccaagtcatg ccactactta tttgtcattg tctgatagat ttaagactag
     2701 tggtgacttg gctgttcaaa taggtaacaa caatgtttgt acctatgaac atgtgatttc
     2761 atatatgggt ttcaggtttg atgttagcat gcctggtagt catagtttgt tctgtactag
     2821 agactttgcc atgcgtcatg tcagaggttg gttaggaatg gatgtggaag gtgcacatgt
     2881 cacaggtgac aatgttggca ctaatgtacc tctacaagtt ggtttttcca atggtgttga
     2941 ttttgtagct caacctgaag gttgtgttct aacaaacact ggcagtgttg taaaacctgt
     3001 tcgtgctcgt gcaccacctg gagaacaatt cactcacatt gtacctctgt tacgcaaggg
     3061 acaaccttgg agtgtgttga gaaaacgtat tgttcaaatg atagcagatt ttcttgctgg
     3121 ctcatctgat gtactggtgt ttgtactttg ggctggcggt ttagagttga ccactatgcg
     3181 ttattttgtt aagattggag ctgttaaaca ttgccaatgt ggtactgttg caacatgcta
     3241 caattctgtt agtaatgact attgttgctt taaacatgca ttgggctgtg actatgttta
     3301 taatccatat gtcatagata ttcaacaatg gggttatgtt ggttcactct ccactaatca
     3361 ccatgcaatt tgtaatgttc atagaaatga gcatgttgct tctggtgatg ctattatgac
     3421 tagatgtttg gctgtgtatg actgctttgt taagaatgtg gattggtcaa ttacctaccc
     3481 tatgatagct aatgaaaatg ccataaacaa gggcggtcgc actgtgcaga gtcatattat
     3541 gcgtgctgct attaaattgt acaaccctaa agcaatccat gacattggta atcctaaggg
     3601 tattcgttgt gctgtaactg atgccaagtg gtattgttat gacaagaacc ctattaattc
     3661 taatgtgaaa acattggagt atgattacat gacacatggc caaatggatg gcttgtgttt
     3721 gttttggaat tgtaatgtgg atatgtaccc tgaattctca attgtttgca ggtttgacac
     3781 acgtacacga tctacattga accttgaagg tgtaaatggt gggtcattgt atgtcaataa
     3841 tcatgcattt cacactcctg cttatgataa acgtgctatg gctaaattga aaccagcacc
     3901 gtttttctac tatgacgacg gttcatgtga ggttgttcac gatcaagtta actatgttcc
     3961 tttgagagcc actaattgca ttaccaagtg taatattggt ggtgctgtat gttctaagca
     4021 cgctaatctc tatagagcat atgttgagtc atataacatt tttactcaag ctggttttaa
     4081 tatttgggtt cctaccacgt ttgattgtta taatttgtgg cagacattca cagaggtcaa
     4141 tttacaactt aattaagacg cgatcgcggc ctcatgggcc cagctttctt gtacaaagtt
     4201 ggcattataa aaaataattg ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     4261 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     4321 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     4381 gataaaaata tatcatcatg cct
//
