LOCUS       sequence-479309-        3077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-229E-Envelope
FEATURES             Location/Qualifiers
     source          1..3077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(225..324)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(340..357)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(340..356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(349..371)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      420..506
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      598..625
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(784..801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(955..1543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1035..1054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1636..2427)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2858..2874
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2858..2874
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2879..2898
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2879..2897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2915..3014
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtgcatatat gattggtaaa tgtggtacac atttttaatg gggccataaa ctgttctatt
       61 acaaaacata tggcaagtga aacaaagctt aattagttta attattgtaa tacacactag
      121 tagtatcact ataagcacca cacaccagag tagtacatta acaaccaaag catgatcatc
      181 cactagctta aggaaggatc ccatggatcc tggccttgac ggcccttctg cttttttgta
      241 caaagttggc attataaaaa agcattgctc atcaatttgt tgcaacgaac aggtcactat
      301 cagtcaaaat aaaatcatta tttggggccc gagcttaaga ctggccgtcg ttttacaacg
      361 tcgtgactgg gaaaacatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc
      421 aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg
      481 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac
      541 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga
      601 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctggc tagcggtacg
      661 cgtattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      721 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1381 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1561 ttctacgggg tctgacgctc agtggaacga cgcgtaactc acgttaaggg attttggtca
     1621 tgggtggctc gagggttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
     1681 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
     1741 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
     1801 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
     1861 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     1921 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     1981 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     2041 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
     2101 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
     2161 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
     2221 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
     2281 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
     2341 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
     2401 tacttcggcg atcaccgctt ccctcataat gtttaacttt gttttagggc gactgccctg
     2461 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
     2521 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa
     2581 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     2641 gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc actgggttcg
     2701 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga
     2761 ggcatttctg tcctggctgg tctagaggag gcatgatgat atatttttat cttgtgcaat
     2821 gtaacatcag agattttgag acacgggcca gagctgccag gaaacagcta tgaccatgta
     2881 atacgactca ctatagggga tatcagctgg atggcaaata atgattttat tttgactgat
     2941 agtgacctgt tcgttgcaac aaattgatga gcaattattt tttataatgc caactttgta
     3001 caagaaagct gggcccatga ggccgcgatc gcgtcttaat taaggaaatc aataactcgt
     3061 ttagggaaag ggtctat
//
