LOCUS       sequence-47931-e         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  TITLE     NLdDIS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCR2.1-PBGD
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(234..250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(234..250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(247..269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    258..274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(282..288,289..306,307..312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    327..348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(465..482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg
       61 tgatggatat ctgcagaatt cgcccttagg gattcactca ggctctttct gtccggcaga
      121 ttggagagaa aagcctgttt accaaggagc ttgaacatgc aagggcgaat tccagcacac
      181 tggcggccgt tactagtgga tccgagctcg gtaccaagct tggcgtaatc atggtcatag
      241 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      301 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      361 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      421 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      481 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      541 ttatccacag aatc
//
