LOCUS       sequence-47932-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     NLdDIS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCR2.1-myco
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(625..641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(625..641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(638..660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    649..665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(673..679,680..697,698..703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    718..739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(856..873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg
       61 tgatggatat ctgcagaatt cgcccttcgg ataacgcttg cgacctatgt attaccgcgg
      121 ctgctggcac atagttagcc atcgctttct gacagggtac cgtcagcctg gaagcatttc
      181 ctcaaccagg tgttctttcc ctataacagc actttacaat ccgaagacct tcatcgtgca
      241 cgctgtgttg ctccatcaag ctttcgctca ttgtggaata ttccctactg ctgcctcccg
      301 taggagtttg ggccgtatct cagtcccaat gtggccgttc agtctctcga cccggctaaa
      361 catcatagtc ttggtaggcc attaccctac caactaacta atgttccgca ccctcatcct
      421 ctagcggagc tttaagggct cctttcacaa cggaatcatg cgattccatg cgtatccggc
      481 attagccaat gtttccattg ggtattccaa tctaaagggt agattaagca cgtgttactc
      541 acccattcgc caagggcgaa ttccagcaca ctggcggccg ttactagtgg atccgagctc
      601 ggtaccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      661 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      721 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      781 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      841 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      901 g
//
