LOCUS       sequence-479399-        2440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty Node C that can be used for the assembly of pEND-Y11 and 
            pEND-Z11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Nevot G, Guell M, Santos-Moreno J
  TITLE     Critical Analysis of Preprints and Inquiry-Based Lessons Improve the
            Synthetic Biology Learning Experience.
  JOURNAL   ACS Synth Biol. 2025 Aug 15;14(8):2878-2884. doi: 
            10.1021/acssynbio.5c00014.
  PUBMED    40814254
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/acssynbio";
            journalName: "ACS Synth Biol"; date: "2025-08-15- 15"; volume: "14";
            issue: "8"; pages: "2878-2884"
COMMENT     Sequence Label: pNC-011
FEATURES             Location/Qualifiers
     source          1..2440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(423..441)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      437..484
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      493..579
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      671..698
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(827..1618)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDDGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1619..1710)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1776..2364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1856..1875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgctc ctaggatgca gtgactcgca catttccccg aaaagtgcca
      181 cctgaattca gaagtattgg taatcgttga aaactcagtc gacgcactta ctgaagacgt
      241 cctattacac tcgtcgttgg aaactgaaga tgcggccgcg gaaacacaga aaaaagcccg
      301 cacctgacag tgcgggcttt ttttttcgac caaaggtagc gaacgacgag tcactgttga
      361 ggataaatac tttctctact aggcgcctgt tacacaggtc ctcagcggcg cgcctgctgc
      421 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      481 tttgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
      541 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      601 gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      661 caaactaagc agaaggccat cctgacggat ggcctttttg cgtttcagat ctaccggtaa
      721 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcat
      781 cgtggccgga tcttgcggcc cctcggcttg aacgaattgt tagacattat ttgccgacta
      841 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
      901 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
      961 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     1021 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     1081 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     1141 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     1201 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     1261 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     1321 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     1381 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     1441 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgt
     1501 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     1561 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatac
     1621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1681 tatttgaatg tatttagaaa aataaacaaa tagctagctc actcggtcgc tacgctccgg
     1741 gcgtgagact gcggcggggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2281 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga
//
