LOCUS       sequence-479406-       11737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for microinjection of nematodes for expression of PDZD8-WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pHM1 PDZD8-WT
FEATURES             Location/Qualifiers
     source          1..11737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     183..200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    317..338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(353..358,359..376,377..383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    391..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     396..418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     415..431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     415..431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8010..8038)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8786..8806
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(8883..8907)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9733..9755)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9855..9874
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9956..9974)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10042..10146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10147..10215,10216..11007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10365..10384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11178..11737,1..29)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11667..11686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
       61 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      301 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      361 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      421 acagctatga ccatgattac gccaagcttg catgcgcggc cgcactgact gggccggcct
      481 gctaaattgc aaccccgtgt ttttgaaaaa atacatcata acagagaaaa ctctttaaaa
      541 tcatattgtt cagccaggtc acagtcaagg acaattttga atttgactca atttcagtta
      601 caggctcgga taacggcgct gccacgacca ccctggcccc ttctgaccca acttgttaaa
      661 tattaatcta aatacaaata agatttagta tgaacattta ctgttcattt tgaatgttgt
      721 ttttttacac attttcgtag ttgaaaattt tgacatattt taagtgttgg aaaagatatg
      781 agacatttga gcgaactcgt ccaaatttgt gaaaatttcc aaaaatcatc gtgaaaaatt
      841 aaaaaacata ttttcagaga aaaggcttaa acatttttga atatgaataa gtaacctttt
      901 ttaaaaatcg gcatgttttt ttctttgaag atcaatattt ttgcgaaagc ctacgatact
      961 aaaaaatatt aacagcaaaa aggtgtaaaa ttaaaaagtg attgtttcat ctgttgacaa
     1021 aagttttgtc aaacaactgg ttttggaatt tatatgcaaa aacctgtaaa aagagtgttt
     1081 attgaatctt ggacttgact tgtttcatcg gactgtaatt tagcaggggc ttaagatagg
     1141 atgaattggt aaagtgataa aatatagcta ttggtatgca aaatattgtt acaattgatt
     1201 actttttggt atcattcacg tttctgaagt taccgccact caaagttaaa taaaataatt
     1261 tttgcaagca tttttgacaa cttaaacgaa tatggcggga ccaaaaaggc catgcctgga
     1321 gctgttattg catgtttttg ataaacttac tgtcatacat cagggtacat aaggtttttg
     1381 atttcacaca aagaaaaaaa aatcgaaaaa aaaacaattt ttttccgttg tcatagttga
     1441 aaaaccctgt tgctaaacaa ttcttctaat ttttcgtggt aactgctaca caatcggatt
     1501 aaggaaacct aattttttct cacatatgtt ggagagcctc gatattcttg aactttttgc
     1561 attcattgtt taatgtttct tcacatagct tcgtatatat tgccagcaat gcaaatcttg
     1621 agccatcggt tgaaatgatt ttgaaaaggt gctcggcata tctgggctga aaaaaaactt
     1681 ttgtttcaac gcattaaaca cttttcgggg ttctgttcca attttttaaa tagttgtaac
     1741 acccgctaga atttccgttt caccaaagaa atctttgttc acagattatt atatatttct
     1801 tttttatgta ttttgtccag gtgatcctaa atgtttaaaa aacgttccag cagcttttag
     1861 tctatacgaa gtatattttt tcttattttg gtttatcgga ggtatgcagt tccaaatgtt
     1921 ggattttcta ccctactaga aatttccttt agttatacaa ttttaattcg aagttgtatc
     1981 ttgaaaagga aaccatcatc aacaacaaat aagcttgaga ctagaattgc taatcgtctg
     2041 caccaaatat gaaattcgtt gaataaatta tataaaacag ttgacaatca taaagtattt
     2101 tacatacata tgtatagtta atgtcatatt agcatattta gtattgagtt aacacgcaga
     2161 aataccaaag aaaccctgtt taattcattt ccatgtttta ttcgctttaa ctagggaatg
     2221 accagaataa gtggagcgat attcaaaaaa aaaaattgta tcggaaagct gacattctct
     2281 actataagaa tatgactgaa atttttgccc attcgggctg gaaatctgaa tggaaattgt
     2341 ttatatgagg ggatccatgg ttgtagttac aattttgaga aaaaagttta aattttaggg
     2401 gaaaagatgt tgaatgaatc attatggtgt tggtcgacac attgatcatg ctgatatcga
     2461 tcagaaaaat taaatttggg cttttatata gtttcgaaaa aacacgttta aatttttgaa
     2521 aaattttacg ttagggtgga attgaacccc agagtcccac catgcttgtc atccactcta
     2581 tccactcggc catctcgctg ttgcaggcag tactaattgg ggatagtgaa aagagttaac
     2641 gtagaatttc agacgtaaaa atttcagatt tccagcccga atgggcaaaa atttcagtca
     2701 tattcttata gtagagaatg tcagctttcc gatacaatat tttttttttg aatatcgctc
     2761 cacttattct ggtcattccc tagtaagttt tttcagtttt accagaaata aaaccactca
     2821 taaaaactat aaactacaca tttaaactgt aaatgttgga aatcattttg accatcaaac
     2881 aatagctatc ggaaaatgca aaggccattc cgtgatggca cgctcttctt ctatccatcg
     2941 ctccttttca acgaaacaca cacacaaacg cagactattg gaaaagaaaa atatagaaaa
     3001 aacctttttt ctccttgtcc cttgtgttga tctcactttt cagtggacgt gtgcagtgtg
     3061 gaggccagag actgtgctga ccgacggacg cgcaggcggt aaacatacgt tgcagtggag
     3121 agtgtagtcc ctcgtcggct ctctgtgtat ttctttcaat gcctgtgtct cggtctatct
     3181 ttgtctctat ctgttttaaa agttttttgc aaagtttcaa tttctttttt attttctctt
     3241 ctttttttgc gcatttattt tttttaaaga gcgctgttct tcaattggaa aacagtataa
     3301 cgatcgaaat aaaaaaaaaa cttgaaatag tatcggaatt gagatggggg caattgaaat
     3361 tgaagacgca acaaaaacat gtttttatcg tctgaaatta tctaaacttt gaatctaacg
     3421 tgtcaatggc tacttttaca gtagactttt tagagtaatg ttaaaaagga taatataggt
     3481 ttttcttcaa actattttgt cacgccgcat atattctttt catattccat tcgcaaagtg
     3541 catgatgtaa caaaaatgaa acgtcataat tttttaatct ttcacacttt ttttgccggc
     3601 cgttaaatca aaatcaaatc aacttaatcc aacacgtgcg ggcttgcaat tttattacac
     3661 cggtgtccta cagcgccatg ttatcttctt tgattagaga tctctcacgc aacatttgac
     3721 attgaaaatt ttgtctccga attgttgtgt acctactttg tcccgatgtc ccaaattttc
     3781 actgataaca agagatatct caactgttct tatcacgttg atacgtagaa cgttttacac
     3841 taaaccaaat tgcagtcccc ttctacgaac cgtttgctaa ttgttttgca tagaacagtg
     3901 atcaatgtgt tgtgctcact agtcaatgat gttcgtctct catttttcct acaacttcac
     3961 tgaaaaggtt ttgaatatct ggtgattgta ggcaatcaca ccacagttaa tggcctggca
     4021 aataatgata agcgtgtgat aaacctcaca aaaatacgcc tagaagtggt gatatcgcta
     4081 gttttgtgtc ctttcgattt ttttcgtgtt gcttttttct gtggataaac ctttgaactt
     4141 tacattttgt tttcagaggc gcgcctctag aggatccccg ggattggcca aaggacccaa
     4201 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
     4261 catggggctg ctgctcatga tcctggcgtc ggccgtgctg ggttccttcc tcacgctcct
     4321 cgcccagttc ttcctgctgt accgcagaca gcccgagccg ccggcggacg aggccgcccg
     4381 cgcgggcgag ggcttccgct acatcaagcc agtgccgggc ctgctcctaa gggagtacct
     4441 ttatggcggc ggccgggatg aggagccctc cggagcggcc cctgagggcg gcgcgacccc
     4501 caccgcggcc cccgagaccc ccgccccgcc gacgcgggag acttgctact tcctcaacgc
     4561 caccatccta ttcctgttcc gggagttgcg ggacaccgcg ctgacccgcc gctgggtcac
     4621 caagaagatc aaggtggagt tcgaggagct gctgcagacc aagacggccg ggcgcctgct
     4681 ggaggggctg agcctgcggg acgtgttcct gggcgagacg gtgcccttca tcaagaccat
     4741 ccggctcgtg cggccagtcg tgccctcggc caccggggag cccgatggcc ctgaagggga
     4801 ggcgctgccc gccgcctgcc ccgaggagct ggccttcgag gcggaggtgg agtacaacgg
     4861 gggcttccac ctggccatcg acgtggacct ggtcttcggc aagtccgcct acttgtttgt
     4921 caagctgtcc cgcgtggtgg gaaggctgcg cttggtcttt acgcgcgtgc ccttcaccca
     4981 ctggttcttc tccttcgtgg aagacccgct gatcgacttc gaggtgcgct cccagtttga
     5041 agggcggccc atgccccagc tcacctccat catcgtcaac cagctcaaga agatcatcaa
     5101 gcgcaagcac accctaccga attacaagat caggtttaag ccgttttttc cataccagac
     5161 cttgcaagga tttgaagaag atgaagagca tatccatata caacaatggg cacttactga
     5221 aggccgtctt aaagttacgt tgttagaatg tagcaggtta ctcatttttg gatcctatga
     5281 cagagaggca aatgttcatt gtacacttga gttaagcagt agtgtttggg aagaaaaaca
     5341 gaggagttct attaagacgg ttgaattaat aaaaggaaat ttacaaagtg ttggacttac
     5401 acttcgtctt gtccagtcaa ctgatgggta tgctgggcac gtcatcattg aaactgtggc
     5461 tccaaactcg cctgctgcaa ttgcagatct tcagcgggga gatcgactta tcgccattgg
     5521 aggtgtgaaa atcacatcaa cactgcaagt gttgaagctt atcaagcagg ctggtgaccg
     5581 agtcctggtg tactatgaaa ggcctgttgg ccagagtaat caaggtgcag tgctgcaaga
     5641 taactttggc cagttggaag aaaacttttt gtcaagctca tgccaatcgg gttatgaaga
     5701 ggaagctgcc gggttgacag tagatactga aagtagagag ctggattctg aatttgaaga
     5761 cttggcaagt gatgtcagag cacaaaatga gttcaaagat gaggcacaat cattaagtca
     5821 tagtcccaaa cgtgttccaa caacactttc tattaaaccc cttggagcta tatcaccagt
     5881 tttaaaccgt aaattagctg taggaagtca cccactacca ccgaaaattc agtccaaaga
     5941 tggaaataaa cctccacccc taaaaacttc tgagataaca gacccagcac aagtgtcaaa
     6001 accaacccaa ggatctgctt tcaaaccacc tgtgccacca cgaccacaag cgaaagttcc
     6061 tttgccttcc gccgatgctc caaatcaggc agaaccagat gttctcgttg aaaagccaga
     6121 gaaggtggtg ccacctcctc ttgtagataa atctgctgaa aagcaagcaa aaaatgtgga
     6181 tgccatagac gatgcagctg cacctaagca atttttagca aagcaagaag tggccaaaga
     6241 tgtcacttca gaaacttcct gccctactaa ggacagttcg gacgaccgtc aaacatggga
     6301 atcatcagaa attctttatc gtaataagct aggaaaatgg acaagaacca gagcatcctg
     6361 tttgtttgac atagaagcct gtcacaggta cttaaacatt gcattgtggt gcagggatcc
     6421 tttcaagttg ggaggtctca tctgtttggg gcatgttagt ttaaaacttg aagatgtggc
     6481 tttaggatgc ctagctacat caaacacgga atacctttcc aaattgagac tggaagcccc
     6541 ctcacctaag gctatagtca ctagaaccgc actacgcaat ctgagtatgc aaaagggatt
     6601 caatgacaaa ttttgctatg gtgacattac tattcacttc aaatatttga aagaaggaga
     6661 atcagaccac catgtagtta ctaacgtaga aaaagaaaaa gaaccccatt tggttgaaga
     6721 agtttctgtt ctccctaaag aggagcaatt tgttggacag atgggtttaa cagaaaacaa
     6781 acacagtttt caggatactc agttccagaa cccaacatgg tgtgactact gtaagaaaaa
     6841 agtttggact aaagcagctt cccagtgtat gttttgtgct tatgtttgcc ataaaaaatg
     6901 tcaagaaaag tgtctagctg agacttctgt ttgtggagca actgataggc gaatagacag
     6961 gacactgaaa aaccttaggc tggaaggaca ggaaaccctc ttaggcctgc ctcctcgtgt
     7021 tgatgctgaa gctagcaagt cagtcaataa aacaacaggt ttgacaaggc atattatcaa
     7081 tactagttct cgtttattaa atttgcgtca agtctctaaa actcgccttt ctgaaccagg
     7141 aaccgatctc gtagaacctt caccaaaaca cacacccaac acgtcagaca acgaaggcag
     7201 tgacacggag gtctgtggtc caaacagtcc ttctaaacgg ggaaacagca caggaataaa
     7261 gttagtgaga aaagagggtg gtctggatga cagtgttttc attgcagtta aagaaattgg
     7321 tcgtgatctg tacaggggct tgcctacaga ggaaaggatc cagaaactag agttcatgtt
     7381 ggataagcta cagaatgaaa ttgatcagga gttggaacac aataattccc ttgttagaga
     7441 agaaaaagag acaactgata caaggaaaaa atcacttctt tctgctgcct tagctaaatc
     7501 aggtgaaagg ctacaagctc taacacttct tatgattcac tacagagcag gcattgaaga
     7561 tatagaaact ttagaaagtc tgtctttaga ccagcactcc aaaaaaataa gcaagtacac
     7621 agatgataca gaagaagacc ttgataatga aataagccaa ctaatagact ctcagccatt
     7681 cagcagcata tcagatgact tatttggccc atccgagtct gtgggtaccg ctgtctcatc
     7741 ctactttcac ctagttaact gcttgtctta aaatctatgc ttctctttag tatctaaaat
     7801 tttcctagaa gcttacaagt atataaatgg tctcttctca ataaaggttg tatatttatt
     7861 catcttattg aatctgccat ttcctcgttt ttgcgagttt atataccttc caattttctt
     7921 tctattgtat tttcaacttc taattttaat tcagggaaac tgctgtaccg gtagaaaaaa
     7981 tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     8041 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     8101 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg gtaagtttaa
     8161 acatatatat actaactaac cctgattatt taaattttca gccaacactt gtcactactt
     8221 tctgttatgg tgttcaatgc ttctcgagat acccagatca tatgaaacgg catgactttt
     8281 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     8341 ggaactacaa gacacgtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
     8401 tttcaggtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     8461 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     8521 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa
     8581 acatgatttt actaactaac taatctgatt taaattttca gaacttcaaa attagacaca
     8641 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     8701 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     8761 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     8821 cacatggcat ggatgaacta tacaaatagc attcgtagaa ttccaactga gcgccggtcg
     8881 ctacccagct ttcttgtaca aagtggtgat atctgagctc cgcatcggcc gctgtcatca
     8941 gatcgccatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     9001 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     9061 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     9121 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     9181 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     9241 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     9301 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc
     9361 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa
     9421 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga
     9481 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     9541 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     9601 aattggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     9661 aaacataact gtttctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga
     9721 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9781 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9841 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     9901 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaat taagggcctc
     9961 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    10021 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10081 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10141 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10201 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    10261 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    10321 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    10381 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10441 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10501 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10561 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10621 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10681 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10741 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10801 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10861 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10921 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10981 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    11041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    11341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    11401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11701 tttcgccacc tctgacttga gcgtcgattt ttgtgat
//
